Virusi ya Monkeypox (MPV) Nucleic Acide Detection Kit
IRIBURIRO
Iki gikoresho gikoreshwa mugushakisha vitro zujuje ubuziranenge bwo gukekwaho virusi ya Monkeypox (MPV), indwara zanduye hamwe nizindi ndwara zigomba gupimwa kwandura virusi ya Monkeypox.
Igikoresho gikoreshwa mugushakisha gene ya f3L ya MPV mumatongo no mu mazuru.
Ibisubizo by'ibizamini by'iki gitabo ni ibyavuzwe gusa kandi ntibigomba gukoreshwa nk'igipimo cyonyine cyo gusuzuma indwara. Birasabwa gukora isesengura ryuzuye ryimiterere ishingiye kumavuriro yumurwayi
kwigaragaza nibindi bizamini bya laboratoire.
Gukoresha
| Ubwoko bw'isuzuma | umuhogo no mu mazuru |
| Ubwoko bw'ikizamini | Ubwiza |
| Ibikoresho by'ibizamini | PCR |
| Ingano yububiko | 48ibizamini / agasanduku |
| Ubushyuhe bwo kubika | 2-30 ℃ |
| Ubuzima bwa Shelf | Amezi 10 |
IBIKURIKIRA
Ihame
Iki gikoresho gifata urutonde rwihariye rwa MPV f3L nkakarere kagenewe. Ikirangantego nyacyo cya fluorescence PCR hamwe na acide nucleic tekinoroji yo kurekura byihuse ikoreshwa mugukurikirana aside nucleic virusi binyuze muguhindura ibimenyetso bya fluorescence yibicuruzwa byongerewe imbaraga. Sisitemu yo gutahura ikubiyemo igenzura ryimbere ryimbere, rikoreshwa mugukurikirana niba muri PCR harimo inhibitor za PCR cyangwa niba selile ziri murugero zafashwe, zishobora gukumira neza ibintu bibi bibi.
INGINGO Z'INGENZI
Igikoresho kirimo reagent zo gutunganya ibizamini 48 cyangwa kugenzura ubuziranenge, harimo ibice bikurikira:
Reagent A.
| Izina | Ibice nyamukuru | Umubare |
| Kumenya MPV reagent | Umuyoboro wa reaction urimo Mg2 +, f3L gene / Rnase P primer probe, reaction ya reaction, Taq ADN enzyme. | Ibizamini 48 |
ReagentB
| Izina | Ibice nyamukuru | Umubare |
| MPV Kugenzura neza | Harimo intego ya MPV | 1 tube |
| MPV Kugenzura nabi | Hatariho intego ya MPV | 1 tube |
| ADN irekura reagent | Reagent irimo Tris, EDTA na Triton. | 48pc |
| Reonstonstitution reagent | DEPC yatunganije amazi | 5ML |
Icyitonderwa: Ibice byimibare itandukanye ntishobora gukoreshwa muburyo bumwe
【Imiterere yo kubika hamwe nubuzima bwa Shelf】
1.Ragent A / B irashobora kubikwa kuri 2-30 ° C, kandi igihe cyo kubaho ni amezi 10.
2. Nyamuneka fungura igifuniko cya test tube gusa mugihe witeguye gukora ikizamini.
3.Ntukoreshe ibizamini byo kwipimisha kurenza itariki izarangiriraho.
4.Ntugakoreshe umuyoboro wamenyekanye.
【Igikoresho gikoreshwa】
Bikwiranye na LC480 sisitemu yo gusesengura PCR, Gentier 48E Sisitemu yo gusesengura PCR yikora, sisitemu yo gusesengura ABI7500 PCR.
【Icyitegererezo】
1.Ubwoko bw'icyitegererezo bukoreshwa: umuhogo wo mu muhogo.
2.Icyitegererezo:Nyuma yo kugenzurwa, birasabwa gukoresha umuyoboro usanzwe wa saline cyangwa Virusi yo kubika virusi yakozwe na biologiya ya Hangzhou Testsea kugirango ikusanyirizwe hamwe.
umuhogo:ohanagura toni ya faryngeal byombi hamwe nurukuta rwinyuma rwa faryngeal hamwe na swabable sterile sampling swab, wibiza swab mu muyoboro urimo igisubizo cya 3mL, guta umurizo, no gukomera umupfundikizo.
3.Urugero rwo kubika no gutanga:Ingero zigomba gupimwa zigomba gupimwa vuba bishoboka. Ubushyuhe bwo gutwara abantu bugomba kubikwa kuri 2 ~ 8 ℃ .Urugero rushobora kugeragezwa mu masaha 24 rushobora kubikwa kuri 2 ℃ ~ 8 ℃ kandi niba ingero zidashobora gupimwa mu masaha 24, zigomba kubikwa munsi cyangwa zingana. kugeza kuri -70 ℃ (niba nta bubiko bwa -70 ℃, burashobora kubikwa kuri -20 ℃ by'agateganyo), irinde gusubiramo
gukonjesha no gukonja.
4.Icyegeranyo gikwiye cyo gukusanya, kubika, no gutwara abantu ni ngombwa mu mikorere yiki gicuruzwa.
【Uburyo bwo Kwipimisha】
1.Urugero rwo gutunganya no kongeramo icyitegererezo
1.1 Gutunganya icyitegererezo
Nyuma yo kuvanga igisubizo cyavuzwe haruguru hamwe nicyitegererezo, fata 30μL yicyitegererezo muri ADN irekura reagent hanyuma ubivange neza.
1.2 Kuremera
Fata 20μL ya reagent yo kwiyubaka hanyuma uyongere kuri reagent ya MPV, ongeramo 5μL yicyitegererezo cyatunganijwe haruguru (Igenzura ryiza hamwe nigenzura ribi bizakorwa bigereranywa nicyitegererezo), upfundikire igituba, centrifuge kuri 2000rpm kuri 10 amasegonda.
2. Kwiyongera kwa PCR
2.1 Shyiramo plaque ya PCR yateguwe kubikoresho bya fluorescence PCR, kugenzura nabi no kugenzura neza bizashyirwaho kuri buri kizamini.
2.2 Gushiraho umuyoboro wa Fluorescent ：
1) Hitamo umuyoboro wa FAM kugirango umenye MPV ；
2) Hitamo umuyoboro wa HEX / VIC kugirango ugenzure imbere gene ；
3.Isesengura ryibisubizo
Shyira umurongo shingiro hejuru yumwanya muremure wo kugenzura nabi fluorescent curve.
4. Kugenzura ubuziranenge
4.1 Igenzura ribi ： Nta Ct agaciro yagaragaye muri FAM channel HEX / VIC, cyangwa Ct ＞ 40 ；
4.2 Igenzura ryiza ： Muri FAM channel HEX / VIC umuyoboro, Ct≤40 ；
4.3 Ibisabwa haruguru bigomba kuba byujujwe mubigeragezo bimwe, bitabaye ibyo ibisubizo byikizamini bikaba bitemewe kandi ubushakashatsi bugomba gusubirwamo.
【Gabanya agaciro】
Icyitegererezo gifatwa nkicyiza iyo: Intego ikurikirana Ct≤40, Imbere yo kugenzura imbere Ct≤40.
【Ibisobanuro】
Igenzura rimaze gutambuka, abayikoresha bagomba kugenzura niba hari umurongo wa amplification umurongo kuri buri cyitegererezo mu muyoboro wa HEX / VIC, niba uhari hamwe na Ct≤40, byerekanaga ko gen igenzura imbere yongerewe neza kandi iki kizamini gifite ishingiro. Abakoresha barashobora gukomeza gukurikirana isesengura:
3.Kurugero hamwe na amplification ya gen igenzura imbere byananiranye (HEX / VIC
umuyoboro, Ct ＞ 40, cyangwa nta amplification curve), umutwaro muke wa virusi cyangwa kubaho kwa PCR inhibitor bishobora kuba impamvu yo gutsindwa, ikizamini kigomba gusubirwamo uhereye kubikusanyamakuru;
4.Kurugero rwiza na virusi yumuco, ibisubizo byo kugenzura imbere ntabwo bigira ingaruka;
Kubitegererezo byageragejwe nabi, igenzura ryimbere rigomba gupimwa neza naho ubundi ibisubizo muri rusange ntibyemewe kandi ikizamini kigomba gusubirwamo, guhera kumurongo wo gukusanya icyitegererezo.
Amakuru yimurikabikorwa
Icyemezo cy'icyubahiro
Umwirondoro w'isosiyete
Twebwe, Hangzhou Testsea Biotechnology Co., Ltd nisosiyete ikora ibijyanye n’ibinyabuzima byihuta cyane mu bushakashatsi, guteza imbere, gukora no gukwirakwiza ibikoresho byipimishije muri vitro bisuzumwa (IVD) n'ibikoresho by'ubuvuzi.
Ikigo cyacu ni GMP, ISO9001, na ISO13458 byemewe kandi dufite icyemezo cya CE FDA. Ubu turategereje gufatanya namasosiyete menshi yo mumahanga mugutezimbere.
Dutanga ibizamini byuburumbuke, ibizamini byindwara zandura, ibizamini byo gukoresha ibiyobyabwenge, ibizamini byerekana umutima, ibizamini byerekana ibimenyetso, ibizamini by’ibiribwa n’umutekano ndetse n’ibizamini by’indwara z’inyamaswa, byongeye kandi, ikirango cyacu TESTSEALABS cyamenyekanye cyane ku masoko yo mu gihugu ndetse no hanze yacyo. Ibiciro byiza kandi byiza bidushoboza gufata hejuru ya 50% imigabane yimbere mugihugu.
Gutunganya ibicuruzwa
1.Witegure
2.Gupfukirana
3.Ibice byose
4.Kata umurongo
5.Iteraniro
6.Gapakira imifuka
7.Funga ibifuka
8.Pakira agasanduku
9.Ikibazo
Irinde ibyago bishya: Witegure nonaha nkuko Monkeypox ikwirakwira
Ku ya 14 Kanama, Ishami ry'Umuryango w'Abibumbye ryita ku Buzima (OMS) ryatangaje ko icyorezo cya monkeypox kigize "Ubuzima rusange bwihutirwa bw’ibibazo mpuzamahanga." Ni ku nshuro ya kabiri OMS itanga urwego rwo hejuru rwo gukangurira abantu kwirinda icyorezo cya monkeypox kuva muri Nyakanga 2022.
Kugeza ubu, icyorezo cya monkeypox cyakwirakwiriye muri Afurika kugera mu Burayi no muri Aziya, aho byagaragaye ko muri Suwede no muri Pakisitani.
Nk’uko imibare iheruka gutangwa na CDC yo muri Afurika ibivuga, muri uyu mwaka, ibihugu 12 bigize Umuryango w’ubumwe bw’ibihugu by’Afurika byerekanye ko abantu 18.737 banduye monkeypox, harimo 3,101 byemejwe, 15,636 bakekwaho icyaha, n’impfu 541, aho impfu za 2.89%.
01 Monkeypox ni iki?
Monkeypox (MPX) ni virusi ya zoonotic virusi iterwa na virusi ya monkeypox. Irashobora kwanduza inyamaswa gushika ku bantu, no hagati y'abantu. Ibimenyetso bisanzwe birimo umuriro, guhubuka, na lymphadenopathie.
Virusi ya monkeypox yinjira cyane cyane mumubiri wumuntu binyuze mumitsi ndetse nuruhu rwacitse. Inkomoko yanduye harimo indwara ya monkeypox nimbeba zanduye, inkende, nizindi primates zitari abantu. Nyuma yo kwandura, igihe cyo gukuramo ni iminsi 5 kugeza 21, mubisanzwe iminsi 6 kugeza 13.
Nubwo abaturage muri rusange bashobora kwandura virusi ya monkeypox, hari urwego runaka rwo kwirinda kwambukiranya monkeypox ku bakingiwe ibicurane, bitewe na genetike na antigenic bihuza virusi. Kugeza ubu, monkeypox ikwirakwira cyane cyane mu bagabo baryamana n'abagabo binyuze mu mibonano mpuzabitsina, mu gihe ibyago byo kwandura abaturage muri rusange bikomeje kuba bike.
02 Iyi ndwara ya Monkeypox itandukaniye he?
Kuva umwaka watangira, ikibazo nyamukuru cya virusi ya monkeypox, "Clade II," cyateje icyorezo kinini ku isi. Igiteye impungenge, umubare w'imanza zatewe na "Clade I," ukabije kandi ufite umubare munini w'abahitanwa nawo, uragenda wiyongera kandi byemejwe hanze y'umugabane wa Afurika. Byongeye kandi, guhera muri Nzeri umwaka ushize, ibintu bishya, byica kandi byoroshye kwanduzwa. "Clade Ib, "yatangiye gukwirakwira muri Repubulika Iharanira Demokarasi ya Kongo.
Ikintu kigaragara muri iki cyorezo ni uko abagore n’abana bari munsi y’imyaka 15 aribo bibasiwe cyane.
Imibare irerekana ko hejuru ya 70% by’abanduye indwara ziri mu barwayi bari munsi y’imyaka 15, kandi mu bantu bapfa, iyi mibare yazamutse igera kuri 85%. Ikigaragara ni ukoumubare w'abana bapfa ku bana wikubye inshuro enye ugereranije n'abantu bakuru.
03 Ni izihe ngaruka zo kwanduza Monkeypox?
Bitewe nigihe cyubukerarugendo n’imikoranire mpuzamahanga, ibyago byo kwanduza imipaka ya virusi ya monkeypox birashobora kwiyongera. Nyamara, virusi ikwirakwizwa cyane cyane no kumarana igihe kirekire, nko gukora imibonano mpuzabitsina, guhuza uruhu, no guhumeka hafi cyangwa kuvugana nabandi, bityo ubushobozi bwayo bwo kwanduza umuntu ku giti cye.
04 Nigute wakwirinda Monkeypox?
Irinde guhuza ibitsina nabantu badafite ubuzima bwiza. Abagenzi bagomba kwitondera icyorezo cya monkeypox mubihugu byabo ndetse no mukarere kabo kandi bakirinda guhura nimbeba na primates.
Niba imyitwarire ishobora guhura cyane, iyikurikirane ubuzima bwawe muminsi 21 kandi wirinde guhura nabandi. Niba hagaragaye ibimenyetso nko guhubuka, ibisebe, cyangwa umuriro, shakisha ubuvuzi bwihuse kandi ubimenyeshe umuganga imyitwarire iboneye.
Niba umwe mu bagize umuryango cyangwa inshuti basanze arwaye monkeypox, fata ingamba zo kwirinda, wirinde guhura n’umurwayi, kandi ntukore ku bintu umurwayi yakoresheje, nk'imyenda, uburiri, igitambaro, n'ibindi bintu bwite. Irinde gusangira ubwiherero, kandi ukarabe intoki kandi uhumeka ibyumba.
Monkeypox Gusuzuma Reagents
Indwara ya Monkeypox isuzuma ifasha kwemeza kwandura mugutahura virusi ya virusi cyangwa antibodi, bigafasha ingamba zo kwigunga no kuvura, kandi bigira uruhare runini mukurwanya indwara zanduza. Kugeza ubu, Anhui DeepBlue Medical Technology Co., Ltd yateguye reagent zikurikira zo gusuzuma monkeypox:
Ikizamini cya Monkeypox Antigen: Koresha uburyo bwa zahabu ya colloidal mugukusanya ingero nka oropharyngeal swabs, nasopharyngeal swabs, cyangwa uruhu rusohoka kugirango bumenye. Yemeza kwandura muguhitamo antigene za virusi.
Monkeypox Antibody Test Kit: Koresha uburyo bwa zahabu ya colloidal, hamwe nurugero rurimo amaraso yose yimitsi, plasma, cyangwa serumu. Yemeza kwandura mugushakisha antibodies zakozwe numubiri wabantu cyangwa inyamaswa kurwanya virusi ya monkeypox.
Monkeypox Virus Nucleic Acide Yipimisha Kit: Koresha uburyo bwa fluorescent burigihe bwa PCR uburyo, hamwe nicyitegererezo ni lesion exudate. Yemeza kwandura mu kumenya genome ya virusi cyangwa ibice byihariye bya gen.
Ibizamini byo gupima Monkeypox
Kuva mu 2015, reagent ya testsealabs ya monkeypox yo kwisuzumisha yemejwe hakoreshejwe urugero rwa virusi nyayo muri laboratoire z’amahanga kandi byemejwe na CE kubera imikorere ihamye kandi yizewe. Izi reagent zigamije ubwoko butandukanye bw'icyitegererezo, zitanga ibyiyumvo bitandukanye kandi byihariye, zitanga inkunga ikomeye yo kumenya kwandura monkeypox no gufasha kurushaho kurwanya icyorezo. Fro andi makuru yerekeye ibikoresho byo gupima monkeypox, nyamuneka reba: https://www.testsealabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
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