Virusi ya monkeypox (mpv) acide ya aside iricleic
Intangiriro
Ibikoresho bikoreshwa mu kumenya neza kwa vitro ku manza za MonkeyPox (MPV), imanza zihuye n'izindi manza zigomba gusuzumwa kwa virusi ya monkeypox.
Ibikoresho bikoreshwa mu kumenya F3L gene ya MPV mu muhogo ya Swabs n'icyitegererezo cya Nasal Swab.
Ibizamini byibizamini byibi bikoresho ni ibya clinique byemewe kandi ntibigomba gukoreshwa nkibipimo byonyine kubisuzumye. Birasabwa gukora isesengura ryuzuye ryuburyo bushingiye ku ivuriro ry'umurwayi
kwigaragaza no kwipimisha.
Gukoresha
| Ubwoko bwo Gukwirakwiza | Umuhogo Swabs na Nasal Swab |
| Ubwoko bw'ikizamini | Impamyabumenyi |
| Ibikoresho byo kwipimisha | Pcr |
| Ingano ya Pack | 48skubwa / 1 Agasanduku |
| Ubushyuhe bwo kubika | 2-30 ℃ |
| Ubuzima Bwiza | Amezi 10 |
Ibicuruzwa
Ihame
Iyi kikoresho ifata urukurikirane rwihariye rwa MPV F3L GEE NK'Akarere kagenewe. Igihe nyacyo fluorescence ntarengwa PCR ikoranabuhanga rya PCR na Acide ya Nucleic yihuta akoreshwa mugukurikirana acide ya virusi binyuze mu guhindura ibimenyetso bya fluorescence. Sisitemu yo gutahura ikubiyemo kugenzura ubuziranenge, ikoreshwa mugukurikirana niba hari ibibuza PCR mu byitegererezo cyangwa niba selile ziri mu byitegererezo zafashwe, zishobora kubuza neza ibintu bibi.
Ibigize
Ibikoresho birimo gutunganya ibizamini 48 cyangwa kugenzura ubuziranenge, harimo ibice bikurikira:
Reagent a
| Izina | Ibigize | Ingano |
| MPV Kumenya reagent | Umuyoboro wa reaction urimo MG2 +, F3L Gene / RNASE P Primer Probe, reaction buffer, taq dna enzyme. | Ibizamini 48 |
ReagentB
| Izina | Ibigize | Ingano |
| MPV Kugenzura neza | Ikubiyemo imyigaragambyo ya MPV | Umuyoboro 1 |
| MPV Igenzura ribi | Hatariho gahunda ya MPV | Umuyoboro 1 |
| ADN YASOHOTSE | Reagent irimo Tris, EDTA na Triton. | 48pcs |
| Gusubiramo reagent | Dedec amazi yafashwe | 5ml |
Icyitonderwa: Ibigize imibare itandukanye ntibishobora gukoreshwa muburyo bumwe
【Imiterere yo kubika hamwe nubuzima bwa filf】
1.Icyiciro A / B kirashobora kubikwa kuri 2-30 ° C, nubuzima bwagaciro ni amezi 10.
.
3.Ntukoreshe ibizamini birenga itariki izarangiriraho.
4.Ntugukoreshe umuyoboro wo gutahura.
【Igikoresho gikurikizwa】
Birakwiye kubijyanye na sisitemu yo gusesengura LC480 PCR, Casier 48E Isesengura rya PCR Automatic, Isesengura rya PCR byikora, ABI7500 PCR gusesengura sisitemu.
【Ibisabwa】
1.Biza ubwoko bwicyitegererezo: Umuhogo Swabs Ingero.
2.Umuti wa siporo:Nyuma yo kugenzurwa, birasabwa gukoresha umuyoboro usanzwe cyangwa virusi wo kubungabunga imiyoboro ya Hangzhou Dingssea ibinyabuzima byerekana icyitegererezo.
Umuhogo Swab:Ihanagura amatsinda ya faryngel ya Ifaringel hamwe ninyuma ya Pharyngeal hamwe na Sterile Spile Swab, mermesse yatsinze igisimba kirimo igisubizo cya 3ml kirimo igisubizo cya 3ml kirimo icyitegererezo cya 3ml kirimo igifuniko.
3.Ibikoresho no gutanga:Ingero zigeragezwa zigomba kugeragezwa vuba bishoboka. Ubushyuhe bwo gutwara abantu bugomba kubikwa kuri 2 ~ 8 ℃ .impapuro zishobora kugeragezwa mumasaha 24 urashobora kubikwa kuri 2 ℃ ~ 8 ℃ kandi niba ibyitegererezo bidashobora kugeragezwa mugihe cyamasaha 24, bigomba kubikwa munsi cyangwa bingana Kuri -70 ℃ (niba nta bubiko bwa -70 ℃, birashobora kubikwa kuri -20 ℃ by'agateganyo), irinde gusubiramo
gukonjesha no gutunganya.
4. Icyegeranyo cy'icyitegererezo, kubika, no gutwara abantu ni ngombwa mu mikorere yiki gicuruzwa.
【Uburyo bwo Kwipimisha】
1.Komera gutunganya no kongeramo icyitegererezo
1.1 Gutunganya Icyitegererezo
Nyuma yo kuvanga igisubizo cyavuzwe haruguru hamwe ningero, fata 30μl yicyitegererezo muri ADN urekura umuyoboro wa ADN hanyuma uyivange neza.
1.2
Take 20μL of the reconstitution reagent and add it to the MPV detection reagent, add 5μL of the above processed sample (The positive control and negative control shall be processed in parallel with the samples), cover the tube cap, centrifuge it at 2000rpm for 10 amasegonda.
2. PCR Yongerewe
2.1 Gupakira plate ya PCR yateguye / igituba cya fluorescence pcr, kugenzura nabi no kugenzura neza bigomba gushyirwaho kuri buri kizamini.
2.2 Umuyoboro wa Fluorescent:
1) Hitamo umuyoboro wa Fami ya MPV;
2) Hitamo umuyoboro wa Hex / Vic wo kugenzura Imbere Kumenya Imbere;
3.Gukunda isesengura
Shiraho umurongo use hejuru yubusobanuro bwisumbuye ya fluorescent yo kuyobora.
4.ubugenzuzi
4.1 Igenzura ribi: Nta kamaro ka CT kagaragaye muri Fam, Hex / Umuyoboro, cyangwa CT> 40;
4.2 Igenzura ryiza: Muri Fam, Hex / Umuyoboro wa Hex / Vic, CT≤40;
4.3 Ibisabwa byavuzwe haruguru bigomba kunyurwa muburyo bumwe, ubundi ibisubizo byikizamini bitemewe kandi igeragezwa rigomba gusubirwamo.
【Gukata agaciro】
Icyitegererezo gifatwa nkibyiza mugihe: intego ikurikiranye CT≤40, Igenzura ryimbere Gene Ct≤40.
【Ibisubizo Ibisobanuro】
Igenzura ryiza rimaze kurengana, abakoresha bagomba kugenzura niba hari umurongo wo kwanga kuri buri cyitegererezo muri hex / vic hand hamwe na CT≤40, byerekanaga kose isese yimbere irose kandi iki kizamini cyihariye gifite ishingiro. Abakoresha barashobora gukomeza gusesengura isesengura:
3.Kuko ingero hamwe no kongera igenzura ryimbere gene yananiwe (Hex / Vic
Umuyoboro, CT> 40, cyangwa nta murongo wa Amplifificagarafiya), umutwaro wa virusike cyangwa kubaho kwa PCR bishobora kuba impamvu yo gutsindwa, ikizamini kigomba kuva mu cyegeranyo kimwe;
4.Kubera urugero rwiza n'umuco umuco, ibisubizo byo kugenzura imbere ntibigira ingaruka;
Kubisobanuro byageragejwe, igenzura ryimbere rigomba kwipimisha ibyiza ubundi ibisubizo rusange ntibishoboka kandi ikizamini gikeneye gusubirwamo, guhera ku ntambwe yo gukusanya ingendo
Imurikagurisha
Icyemezo cy'Icyubahiro
Umwirondoro wa sosiyete
Twe, Hangzhou Disseea biotechnology Co., Ltd ni isosiyete ikora ibinyabuzima ikura byihariye mu bushakashatsi, ikura, ikwirakwizwa no gukwirakwiza ibikoresho byateye imbere (ivd) ibizamini n'ibikoresho by'ubuvuzi.
Ikigo cyacu ni GMP, ISO9001, ISO1458 byemewe kandi dufite CE FDA. Noneho dutegereje gufatanya n'amasosiyete menshi yo mumahanga yo guterwa.
Dutanga ikizamini cy'uburumbuke, ibizamini byanduza ibiyobyabwenge, ibizamini by'ibiyobyabwenge, ikizamini cya mardiac, ibizamini by'ibizamini n'ibizamini byindwara byamenyekanye mu masoko yo mu rugo ndetse no mu mahanga. Ubwiza bwiza nibiciro byiza bidushoboza gufata imigabane irenga 50%.
Inzira y'ibicuruzwa
1.Gepare
2.cover
3.Cmerane
4.Kunte
5.Abashimishije
6.Gukemura hejuru
7.Gusare
8.Pack agasanduku
9.Gucanamo
Irinde ibyago bishya: Witegure nonaha nka monkeypox ikwirakwira
Ku ya 14 Kanama Umuryango w'ubuzima ku isi (NINDE) watangaje ko icyorezo cya monkeypox kigizwe n '"ibihimbano rusange byihutirwa byo guhangayikishwa mpuzamahanga." Ni ku nshuro ya kabiri watanze urwego rwo hejuru rwo kuba maso ku bijyanye no gusohoka muri monkeypox kuva 20 Nyakanga.
Kugeza ubu, icyorezo cya monkeypox cyakwirakwiriye muri Afurika mu Burayi no muri Aziya, hamwe na Aziya, hamwe n'imanza zemejwe na Suwede na Pakisitani.
Nk'uko amakuru aheruka muri CDC ya Afurika, uyu mwaka, ibihugu 12 bigize Umuryango w'ubumwe bw'Afurika byatangaje ko imanza 18.737, harimo n'abantu 5,636 bakekwaho, hamwe n'abaciriritse ba 2,89%.
01 monukeypox ni iki?
Monkeypox (MPX) ni indwara ya Zonotic ya vinototic yatewe na virusi ya monkeypox. Irashobora koherezwa mu nyamaswa kubantu, ndetse no hagati yabantu. Ibimenyetso bisanzwe birimo umuriro, guhubuka, na lymphangoopathy.
Virusi ya monkeypox yinjira cyane cyane ku mubiri w'umuntu binyuze mu muco wa mucous no ku ruhu rwacitse. Inkomoko yo kwandura harimo inkunga ya monkeyPox hamwe n'impande zanduye, inkende, n'abandi bantu ba mbere. Nyuma yo kwanduza, igihe cyo kwinjiza ni iminsi 5 kugeza 21 kugeza 21 kugeza kuri 6 kugeza 13.
Nubwo abaturage muri rusange bashobora kwibasirwa na virusi ya monkeypox, hari urwego runaka rwo gukumira Monkeypox kuba barakingiwe ibicurane, kubera ibipimo ngenderwaho na antigenic hagati ya virusi. Kugeza ubu, monkeypox ikwirakwira cyane cyane mu bagabo bakora imibonano mpuzabitsina n'abagabo binyuze mu guhuza imibonano mpuzabitsina, mu gihe ibyago byo kwandura abaturage bakomeje kuba hasi.
02 Iyi monkeypox yo hejuru imeze ite?
Kuva umwaka utangiye, umuco w'ingenzi wa virusi ya monkeypox, "wateye isi yose yateje isi yose. Ushishikajwe, umubare w'imanza uterwa na "Clade I," ukomera cyane kandi ufite igipimo cyo hejuru, kiriyongera kandi cyemejwe hanze y'umugabane wa Afurika. Byongeye kandi, kuva muri Nzeri umwaka ushize, uruhinja rushya, rwica kandi rworoshye rwoherezwa, "Clade Ib, "Yatangiye gukwira muri Repubulika Iharanira Demokarasi ya Kongo.
Ikintu kigaragara cyibi cyorezo nuko abagore nabana bari munsi yimyaka 15 bafite ingaruka cyane.
Amakuru yerekana ko inshuro zirenga 70% ziba mubarwayi bari munsi yimyaka 15, kandi mubibazo byica, iyi mibare izamuka kuri 85%. Ikigaragara,Igipimo cyica abana ni inshuro enye zirenze abantu bakuru.
03 Ni izihe ngaruka zo kwanduza monukeypox?
Kubera ibihe byubukerarugendo no gukora imikoranire mpuzamahanga, ibyago byo kwanduza imipaka virusi ya monkeypox irashobora kwiyongera. Ariko, virusi ahanini ikwirakwira hafi yo guhura cyane cyane, nko guhuza ibitsina, guhuza uruhu, no guhumeka neza cyangwa kuvugana nabandi, kuburyo ubushobozi bwumuntu-imbonankubone ni intege nke.
04 Nigute wakubuza Monkeypox?
Irinde guhuza ibitsina nabantu bafite ubuzima bwiza. Abagenzi bagomba kwitondera impera za monkeypox mu bihugu bigana no ku turere tujya no kwirinda guhura n'imbeba na primates.
Niba imyitwarire yo mu rwego rwo hejuru ibaho, kwikurikirana ubuzima bwawe iminsi 21 kandi irinde guhura cyane nabandi. Niba ibimenyetso nko guhubuka, ibisebe, cyangwa umuriro bigaragara, shakisha ubuvuzi vuba kandi ubimenyeshe umuganga w'imyitwarire.
Niba umwe mu bagize umuryango cyangwa inshuti imwe muri MonkeyPox, fata ingamba zo gukingira, irinde guhura na hafi n'umurwayi wakoresheje, kandi ntukore ku kintu umurwayi yakoresheje, nk'imyenda, uburiri, ikindi kintu. Irinde gusangira ubwiherero, kandi woza intoki kandi ibyumba bya ventilate.
MonkeyPox reagent
Monikeypox isuzuma isuzuma ryemeza kwandura antigons cyangwa antibodies, bituma habaho ingamba zikwiye zo kwigunga no gufata ingamba zifatika mu kugenzura indwara zanduza. Kugeza ubu, Anhui MaltalBue Technology Co., Ltd. yateguye ibikurikira bya monkeypox ikurikira:
MonkeyPox antigen ikizamini: Koresha uburyo bwa zahabu ya Colloidal kugirango akusanye ingero nkicyatsi cya oropharyntual, swab ya saropharyntual, ibishanga bya Nasopharyngeal, cyangwa uruhu rwibutsa, cyangwa uruhu rwo guhinga gukundana. Yemeje kwandura kumenya ahari habaho antimans.
MonkeyPox antibody antibods ikiganiro: Koresha uburyo bwa zahabu ya Colloidal, hamwe ninshuro zishira hamwe amaraso yose, plasma, cyangwa serumu. Yemeje kwandura kumenya antibodies ikozwe numuntu cyangwa inyamanswa kuri virusi ya monkeypox.
Ibizamini bya monkeypox virusi ya acid nucleic: ikoresha igihe nyacyo cya PCR uburyo bwa pcr, hamwe nicyitegererezo ni ugutangaza. Yemeza kwandura kumenya genome ya virusi cyangwa ibice byihariye bya gene.
Ibicuruzwa bya Disseaalabs 'monkeypox igerageza ibicuruzwa
Kuva muri 2015, ibizamini bya monikeypox bisuzumwe ukoresheje virusi nyayo muri Laboratoire z'amahanga kandi ko yemejwe kubera imikorere yabo ihamye kandi yizewe. Ibi bikoresho bigamije ubwoko bwibitekerezo bitandukanye, bitanga urwego rutandukanye kandi rwihariye, rutanga inkunga ikomeye yo kumenya no gufasha neza muburyo bwiza bwo kuvugurura. Shobuja andi makuru yerekeye ibikoresho bya monkeypox
Uburyo bwo kugerageza
Gukoresha Swab gukusanya Pus kuva pussule, uyivange neza muribuffer, hanyuma usabe ibitonyanga bike mu ikarita y'ibizamini. Ibisubizo birashobora kuboneka mugihe gito gusa.
