ICH-CPV-CDV IgG igikoresho

CANINE INFECTIOUS HEPATITIS / PARVO VIRUS / DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ICH / CPV / CDV IgG test kit) yagenewe gusuzuma igice cya kabiri cyo gusuzuma imbwa IgG antibody ya virusi ya Canine yanduye (ICH), Canine Parvo Virusi. na virusi ya Canine Distemper (CDV).

KIT

KUBONA N'AMAHAME

Hano hari ibice bibiri byapakiwe muri buri karitsiye: Urufunguzo, rushyirwa hamwe na desiccant mugice cyo hasi gifunze hamwe na fayili ikingira, hamwe no gukemura ibisubizo, bigashyirwa ukundi mubice byo hejuru bifunze hamwe na fayili ikingira.

Buri karitsiye ikubiyemo reagent zose zikenewe mugupima icyitegererezo. Muri make, mugihe Urufunguzo rwinjijwemo hanyuma rugashyirwa muminota mike mugice cyo hejuru 1, aho hashyizwemo urugero rwamaraso, antibodiyite yihariye ya IgG mumaraso yamenetse, niba ihari, izahuza na ICH, CPV cyangwa CDV recombinant antigens yimuwe ahantu hatandukanye kuri Urufunguzo rwinjijwe. Noneho Urufunguzo ruzimurwa mugice gisigaye cyo hejuru mugihe cyagenwe intambwe ku yindi. Antibodiyite zihariye za IgG ziri ahantu hazashyirwa ikimenyetso hejuru yicyiciro cya 3, kirimo anti-canine IgG enzyme conjugate kandi ibisubizo byanyuma byerekanwe nkibibara byijimye-ubururu kuri Urufunguzo bizatezwa imbere hejuru

igice cya 6, kirimo substrate. Kubisubizo bishimishije, intambwe yo gukaraba iratangizwa. Mu gice cyo hejuru 2, IgG itagira umupaka nibindi bintu biri mu cyitegererezo cyamaraso bizavaho. Mugice cyo hejuru 4 na 5, bidafite umupaka cyangwa

birenze anti-canine IgG enzyme conjugate izavaho bihagije. Mugusoza, mugice cyo hejuru 7, chromosome irenze ikura muri substrate hamwe na enzyme conjugate ihambiriye mugice cyo hejuru 6 izavaho. Kwemeza agaciro k'imikorere, poroteyine yo kugenzura itangirwa hejuru cyane kuri Urufunguzo. Ikibanza cyijimye-ubururu kigomba kugaragara nyuma yo kurangiza inzira yo kugerageza.

Ububiko

1. Bika ibikoresho munsi ya firigo isanzwe (2 ~ 8 ℃).

NTUBUNTU KUBUNTU.

2. Igikoresho kirimo ibintu biologiya bidakora. Igikoresho kigomba gukemurwa

no kujugunywa hakurikijwe ibisabwa by’isuku byaho.

UBURYO BWO GUKORA IKIZAMINI

Kwitegura mbere yo gukora ikizamini:

1. Zana igikarito mubushyuhe bwicyumba (20 ℃ -30 ℃） hanyuma ubishyire ku ntebe yakazi kugeza ikirango cyumuriro kurukuta rwa karitsiye kibaye ibara ritukura.

2. Shira impapuro zisukuye ku ntebe y'akazi kugirango ushire Urufunguzo.

3.Gutegura 10μL dispenser hamwe ninama ya 10μL isanzwe.

4. Kuramo hepfo ya aluminiyumu ikingira hanyuma utere Urufunguzo mu gice cyo hasi cya karitsiye ku mpapuro zisukuye.

5. Hagarara neza karitsiye ku ntebe y'akazi hanyuma wemeze ko nimero yo hejuru ishobora kugaragara mu cyerekezo cyiza (kashe ya nimero ikureba). Kanda karitsiye gato kugirango umenye neza ko ibisubizo biri mubice byo hejuru bisubira inyuma.

Gukora ikizamini:

1.Kingura umwirondoro urinda ibice byo hejuru witonze ukoresheje urutoki n'urutoki kuva ibumoso ugana iburyo kugeza GUSA ugaragaza igice cyo hejuru 1.

2.Kora icyitegererezo cyamaraso yapimwe hamwe na dispenser yashizweho ukoresheje inama isanzwe ya 10μL.

Kugerageza serumu cyangwa plasma koresha 5μL.

Mugupima amaraso yose koresha 10μL.

Imiyoboro ya EDTA cyangwa heparin anticoagulant irasabwa plasma no gukusanya amaraso yose.

3. Shyira icyitegererezo mu gice cyo hejuru 1. Noneho uzamure kandi umanure disipanseri ya plunger inshuro nyinshi kugirango ugere ku kuvanga (Umuti wubururu bwerurutse mumutwe mugihe uvanze byerekana kubitsa neza).

4.Fata Urufunguzo na Urufunguzo rwa Urufunguzo ukoresheje urutoki n'urutoki witonze hanyuma winjize Urufunguzo mu gice cyo hejuru 1 (wemeze uruhande rukonje rwa Urufunguzo rureba, cyangwa wemeze ko uruziga ruzengurutse kuri Holder ruri iburyo iyo ureba wowe). Noneho vanga hanyuma uhagarare Urufunguzo mugice cyo hejuru 1 kuminota 5.

5.

6.

7.Garagaza fayili ikingira ubudahwema ugana iburyo kugeza igihe ushyize ahagaragara GUSA 4. Fata Urufunguzo na Holder hanyuma winjize Urufunguzo mu gice cyerekanwe 4. Noneho vanga hanyuma uhagarare Urufunguzo mu gice cyo hejuru 4 kumunota 1.

8.Garagaza fayili ikingira ubudahwema ugana iburyo kugeza igihe ushyize ahagaragara GUSA 5. Fata Urufunguzo na Holder hanyuma winjize Urufunguzo mu gice cyerekanwe 5. Noneho vanga hanyuma uhagarare Urufunguzo mu gice cyo hejuru 5 kumunota 1.

9.Garagaza fayili ikingira ubudahwema ugana iburyo kugeza igihe ushyize ahagaragara GUSA 6. Fata Urufunguzo na Holder hanyuma winjize Urufunguzo mubice byerekanwe 6. Noneho vanga hanyuma uhagarare Urufunguzo mugice cyo hejuru 6 muminota 5.

10.Garagaza fayili ikingira ubudahwema ugana iburyo kugeza igihe ushyize ahagaragara GUSA 7. Fata Urufunguzo na Holder hanyuma winjize Urufunguzo mu gice cyerekanwe 7. Noneho vanga hanyuma uhagarare Urufunguzo mu gice cyo hejuru 7 kumunota 1.

11. Kuramo Urufunguzo mu gice cyo hejuru 7 hanyuma ureke rwume ku mpapuro za tissue mugihe cyiminota 5 mbere yo gusoma ibisubizo.

Inyandiko:

Ntugakore ku ruhande rwubukonje bwimbere yimbere yurufunguzo, aho antigene na proteine ​​igenzura byimuwe (Akarere gashinzwe kugenzura no kugenzura).

Irinde gushushanya Akarere k'Ikizamini no kugenzura wishimikije urundi ruhande rworoshye rw'uruhande rw'imbere rw'urufunguzo ku rukuta rw'imbere rwa buri gice cyo hejuru mugihe uvanze.

Kuvanga, inshuro 10 kuzamura no kumanura Urufunguzo muri buri gice cyo hejuru birasabwa.

GUSA Shyira ahakurikira igice cyo hejuru mbere yo kwimura Urufunguzo.

Nibiba ngombwa, ongeraho ibirango byamatungo yatanzwe kugirango arenze icyitegererezo.

GUSOBANURA IBISUBIZO BY'IKIZAMINI

Reba ibibanza byavuyemo kuri Urufunguzo hamwe na Ibara risanzwe

Ntibyemewe:

NTA ibara ryibara ryijimye-ubururu rigaragara ahabigenewe

Ibibi(-)

NTA ibara ryibara ryijimye-ubururu rigaragara ahantu hageragezwa

Ibyiza (+)

Ibara ryibara ryijimye-ubururu rigaragara ahantu hageragezwa

Imitwe ya antibodi yihariye ya IgG irashobora kugaragazwa ninzego eshatu