Ibicurane by'ibicurane by'ibiguruka H7 Ikizamini cya Antigen

Ibisobanuro bigufi:

Virus Grippe Avian H7 (AIV-H7) ni virusi yandura cyane yibasira inyoni. Rimwe na rimwe, irashobora kurenga inzitizi yubwoko ikanduza abantu, igatera indwara zikomeye zubuhumekero ndetse zikanahitana abantu. UwitekaH7 Cassette Yihuta Yihutanigikoresho cyizewe cyo kwisuzumisha cyagenewe kurubuga byihuse kumenya H7 subtype ya virusi yibicurane by’ibiguruka mu nyoni. Ni ingirakamaro cyane mugusuzuma hakiri kare mugihe cyadutse niperereza rya epidemiologiya.

Iki gicuruzwa cyagenewe kuba cyoroshye kandi cyoroshye, gikwiriye gukoreshwa muri laboratoire, mu mirima, kugenzura gasutamo, no mu bikorwa byo gukumira indwara ku mipaka. Itanga inkunga ikomeye yo gusuzuma no kurwanya ibicurane hakiri kare.

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Ibicuruzwa birambuye

Ibicuruzwa

Ibicuruzwa birambuye:

Ibyiyumvo Byinshi kandi Byihariye
Yashizweho na antibodi yihariye ya monoclonal ya H7 subtype, kwemeza neza no kugabanya kwambukiranya hamwe nubundi bwoko.
Byihuta kandi byoroshye-Gukoresha
Ibisubizo birahari muminota 15 bidakenewe ibikoresho bigoye cyangwa amahugurwa yihariye.
Icyitegererezo Cyinshi Guhuza
Bikwiranye nubwoko butandukanye bwikitegererezo, harimo nasofaryngeal swabs, tracheal swabs, hamwe numwanda.
Ikoreshwa rya Field Porogaramu
Igishushanyo mbonera kandi cyorohereza abakoresha gukora cyiza cyo gukoresha mumirima cyangwa iperereza ryumurima, bigafasha ibisubizo byihuse mugihe cyadutse.

Ihame:

Ikizamini cya H7 Antigen Rapid ni isuzuma ryikingira rya immunochromatografique ikoreshwa kugirango hamenyekane ko H7 antigene ziri mu ngero nka swabs yinyoni (nasofaryngeal, tracheal) cyangwa ibintu bya fecal. Ikizamini gikora hashingiwe ku ntambwe z'ingenzi zikurikira:

Icyitegererezo
Ingero (urugero, nasopharyngeal swab, tracheal swab, cyangwa fecal sample) zegeranijwe kandi zivangwa na lysis buffer kugirango irekure antigene za virusi.
Immune reaction
Antigene ziri murugero zihuza antibodi zihariye zifatanije na nanoparticles ya zahabu cyangwa ibindi bimenyetso byabanje gushyirwaho kaseti yipimisha, bigakora antigen-antibody.
Chromatographic Flow
Icyitegererezo kivanze cyimuka kuri nitrocellulose. Iyo antigen-antibody igeze kumurongo wikizamini (umurongo wa T), ihuza urundi rwego rwa antibodies zidahagarara kuri membrane, bigakora umurongo wikizamini kigaragara. Reagents idahuza ikomeza kwimukira kumurongo ugenzura (C umurongo), byemeza ko ikizamini gifite agaciro.
Ibisobanuro
- Imirongo ibiri (T umurongo + C umurongo):Igisubizo cyiza, cyerekana ko H7 antigene ihari.
- Umurongo umwe (C umurongo gusa):Ibisubizo bibi, byerekana ko nta antigene ya H7 igaragara.
- Nta murongo cyangwa T umurongo gusa:Ibisubizo bitemewe; ikizamini kigomba gusubirwamo hamwe na cassette nshya.