Kete Whakamatau Tere Waa Hipi-Tukutake (Tikanga Koura Koroiti)

Tohunga me te Inenga]
Whakanohoia te reagent me te tauira ki te pāmahana rūma (10~30°C) mo te 15-30 meneti.Me whakahaere nga whakamatautau i te pāmahana rūma (10 ~ 30 ° C) me te nui o te haumākū (haumākū ≤70%) me karo.Ka noho rite tonu te tikanga whakamatautau i raro i nga ahuatanga o te pāmahana me te makuku.
1.Tauira Whakaritenga
1.1Te Whakaritenga o te Tauira Tipa Wai mai i te Mata Mii
（1）Whakamahia te ūkui ki te tango i te wai kiko mai i te mata o te tauira ka whakamatauria, katahi ka rukuhia te kaukau ki te wairewa tangohanga mo te 10 hēkona.Whakanuia pai ki runga me raro, maui me te taha matau, mo te 10-20 hēkona ki te tareka i te tauira ki roto i te otinga ka taea.
（2）Tangohia te miro, ka reri koe ki te tono i te wai tauira.
1.2Meat Chunk Tissue Tauira Whakaritenga
（1）Ma te whakamahi i te kutikuti (kaore i whakauruhia), tapahia he wahi mīti 0.1g (he rite ki te rahi o te soya).Tāpirihia te kohanga mīti ki te wairewa tangohanga ka ruku mō te 10 hēkona.Whakamahia te ūkui ki te kotēhia te mongamonga mīti kia 5-6 ngā wā, me te kōrori i runga, whakararo, mauī, matau mō te 10-20 hēkona.Ka taea e koe te tono i te tauira wai.
2.Whakatupato
（1）Ko tenei reagent he mea noa mo te whakamatautau i te kai mata, i te tukatuka noa ranei i nga taonga kai kore i tunua.
（2）Mehemea he iti rawa te wai ka taapirihia ki te kaari whakamatautau, ka puta he kino kino, he hua kore ranei.
（3）Whakamahia he topata/pipette ki te maka poutū te wai whakamatautau ki roto i te rua tauira o te kaari whakamatautau.
（4）Kare i te paheketanga whakawhiti i waenga i nga tauira i te wa e whakatauira ana.
（5）I te wa e whakamahi ana i te kutikuti hei tapahi i te kiko mīti, me whakarite kia ma te kutikuti kia kore e poke i te takenga kararehe.Ka taea te horoi me te whakamahi ano i nga kutikuti i nga wa maha.
[Whakamaoritanga o nga Hua Whakamatau]
Pai (+): E rua nga rarangi whero ka puta.Ka puta tetahi raina ki te waahi whakamatautau (T), me tetahi atu raina i te waahi whakahaere (C).Ko te tae o te roopu i te waahi whakamatautau (T) ka rereke pea te kaha;ko tetahi ahua e tohu ana he hua pai.
Negative (-): He roopu whero anake ka puta i te waahi whakahaere (C), kaore he roopu e puta mai i te waahi whakamatautau (T).
He muhu: Karekau he roopu whero ka puta ki te waahi whakahaere (C), ahakoa ka puta he roopu ki te waahi whakamatautau (T), kaore ranei.Ka tohu tenei he hua muhu;me whakamahi he riipene whakamatautau hou mo te whakamatautau ano.
He Tohu Hua Pai: Kua kitea nga waahanga takenga hipi i roto i te tauira.
He Tohu Hua Kino: Karekau he wahanga hipi-take i kitea i roto i te tauira.