Nlele Ọrịa Testsea HIV 1/2 Ngwa ule ngwa ngwa

Nnwale Ọrịa Testsea HIV 1/2 Ngwa ngwa ngwa gosipụtara onyonyo

Nkọwa dị mkpirikpi:

Nje Virus Immunodeficiency Human (HIV)bụ nje na-awakpo usoro ahụ ji alụso ọrịa ọgụ, na-ezubere ya kpọmkwemCD4+ T cell(nke a makwaara dị ka mkpụrụ ndụ T-helper), nke dị oke mkpa maka nchekwa nchekwa. Ọ bụrụ na a naghị agwọ ya, nje HIV nwere ike ibute yaEnwetara Immunodeficiency Syndrome (AIDS), ọnọdụ ebe usoro ahụ ji alụso ọrịa ọgụ na-emebi nke ukwuu na enweghị ike ịlụso ọrịa ọgụ na ọrịa ọgụ.

A na-ebute nje HIV n'ụzọ bụ isiọbara, semen, mmiri mmiri ikpu, mmiri mmiri rectal, nammiri ara ara. Ụzọ mbufe a na-ahụkarị gụnyere mmekọ nwoke na nwanyị na-enweghị nchebe, ịkekọrịta agịga emetọru, na ibufe nne na nwa mgbe a na-amụ nwa ma ọ bụ na-enye nwa ara.

Enwere ụdị isi abụọ nke nje HIV:

HIV-1:Ụdị nje HIV kachasị na-agbasa na nke zuru ụwa ọnụ.
HIV-2:Ọ na-adịkarịkarịghị, nke a na-ahụkarị na West Africa, yana a na-ejikọta ya na iji nwayọọ na-aga n'ihu na AIDS.

Iji chọpụta na ọgwụgwọ mbụọgwụgwọ antiretroviral (ART)nwere ike inyere ndị nwere nje HIV aka ibi ndụ ogologo oge, ahụike ahụike yana belata ohere nke ibufe ndị ọzọ.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa:

Mmetụta dị elu na nkọwapụta
Emebere ule a iji chọpụta ma nje HIV-1 na HIV-2 nke ọma, na-enye nsonaazụ a pụrụ ịdabere na ya na obere mmeghachi omume.
Nsonaazụ ngwa ngwa
Nsonaazụ dị n'ime nkeji 15-20, na-enye ohere ịme mkpebi ụlọ ọgwụ ozugbo yana ibelata oge nchere maka ndị ọrịa.
Ọ dị mfe iji
Nhazi dị mfe na nke onye ọrụ, na-achọghị akụrụngwa ma ọ bụ ọzụzụ pụrụ iche. Kwesịrị ekwesị maka ojiji na ntọala ụlọ ọgwụ yana ebe dịpụrụ adịpụ.
Ụdị Nlereanya Na-agbanwe agbanwe
Nnwale ahụ dakọtara na ọbara zuru oke, serum, ma ọ bụ plasma, na-enye mgbanwe na nchịkọta nlele yana ịbawanye ngwa ngwa.
Ngwa mbugharị na ngwa ubi
Kọmpat ma dị fechaa, na-eme ka ngwa ule dị mma maka ntọala nlebara anya, ụlọọgwụ ahụike mkpanaka, na mmemme nyocha ọtụtụ.

Ụkpụrụ:

Nchịkọta ihe atụ
A na-etinye ntakịrị olu nke serum, plasma, ma ọ bụ ọbara zuru ezu n'ime olulu mmiri nlele nke ngwaọrụ nyocha, na-esote mgbakwunye nke ihe nchekwa nchekwa iji malite usoro nyocha.
Mmekọrịta Antigen-Antibody
Nnwale ahụ nwere antigens recombinant maka HIV-1 na HIV-2, bụ ndị na-adịghị emegharị ahụ na mpaghara ule nke akpụkpọ ahụ. Ọ bụrụ na nje nje HIV (IgG, IgM, ma ọ bụ ha abụọ) dị na nlele ahụ, ha ga-ejikọta na antigens dị na akpụkpọ ahụ, na-eme mgbagwoju anya antigen-antibody.
Mbugharị chromatographic
Ihe mgbagwoju anya antigen-antibody na-aga n'akụkụ akpụkpọ ahụ site na ọrụ capillary. Ọ bụrụ na nje nje HIV dị, mgbagwoju anya ga-ejikọta na akara ule (T line), na-emepụta ahịrị na-ahụ anya. Ndị reagents fọdụrụ na-akwaga na ahịrị njikwa (C line) iji hụ na nnwale ahụ ziri ezi.
Nkọwapụta nsonaazụ
- Ahịrị abụọ (akara T + C):Nsonaazụ dị mma, na-egosi ọnụnọ nke nje HIV-1 na/ma ọ bụ nje HIV-2.
- Otu ahịrị (akara C naanị):Nsonaazụ na-adịghị mma, na-egosi enweghị ọgwụ mgbochi nje HIV a na-achọpụta.
- Enweghị ahịrị ma ọ bụ T naanị:Nsonaazụ ezighi ezi, chọrọ nnwale ugboro ugboro.

Ihe mejupụtara:

Ihe mejupụtara	Ọnụ ego	Nkọwapụta
IFU	1	/
Nwalee cassette	1	Akpa foil ọ bụla emechiri emechi nwere otu ngwaọrụ nnwale na otu ihe eji eme ihe
mmịpụta diluent	500μL * 1 Tube * 25	Ihe nchekwa Tris-Cl, NaCl, NP 40, ProClin 300
Ntụpọ dropper	1	/
Swab	1	/

Usoro nnwale:


1. Saa aka gi	2. Lelee ihe dị n'ime ngwa ahụ tupu ịnwale, gụnyere ntinye ngwugwu, kaseti ule, ihe nchekwa, swab.	3. Tinye tube mmịpụta na ebe a na-arụ ọrụ.	4.Kpechapụ ihe mkpuchi aluminom si n'elu nke ntanye mmịpụta nke nwere ihe ntinye ihe.

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5. Jiri nlezianya wepụ swab na-emetụghị ọnụ ọnụ. Tinye ọnụ ọnụ nke swab 2 ruo 3 cm n'ime imi imi ziri ezi. Rịba ama ebe mgbawa nke imi imi. Ị nwere ike inwe mmetụta nke a na mkpịsị aka gị mgbe ị na-etinye imi imi ma ọ bụ lelee. ya n'isi. Na-ete ime ime imi na mmegharị okirikiri ugboro 5 maka opekata mpe 15 sekọnd, Ugbu a were otu imi imi ma tinye ya n'oghere imi ọzọ. Biko jiri ihe nlele were mee nnwale ahụ ozugbo ma emela ya
hapụ ya ka ọ kwụ.

6. Tinye swab na tube mmịpụta. Gbanwee swab maka ihe dị ka 10 sekọnd, gbanwee swab megide tube nke na-emepụta ihe, na-agbanye isi nke swab megide n'ime tube ahụ ka ị na-agbanye akụkụ nke tube ahụ iji hapụ dị ka mmiri mmiri. dị ka o kwere mee site na swab.


7. Wepụ swab na ngwugwu na-emetụghị ihe mgbochi ahụ aka.	8.Mix nke ọma site n'ịgbanye ala nke tube ahụ. Tinye 3 tụlee nke nlele ahụ n'ụzọ ziri ezi n'ime ihe nlele nke ọma nke cassette ule. Gụọ ihe si na ya pụta mgbe minit 15 gasịrị. Rịba ama: Gụọ nsonaazụ n'ime nkeji 20. Ma ọ bụghị ya, a na-akwado arịrịọ maka ule.

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