Nnwale Ọrịa Testsea HCV Ab ngwa ngwa ngwa ngwa

Nnwale Ọrịa Testsea HCV Ab ngwa ngwa ngwa egosipụtara onyonyo

Nkọwa dị mkpirikpi:

Ịba ọcha n'anya Cbụ ọrịa nje nke na-ebuteỌrịa ịba ọcha n'anya C (HCV)nke kacha emetụta imeju. O nwere ike ịkpata ha abụọnnukwunaadịghị ala alaọrịa. Ọrịa HCV na-adịghị ala ala nwere ike ibute nsogbu imeju siri ike, dịkacirrhosis, ọrịa imeju, naimeju ọdịda, na ọ bụ ihe kacha akpata ibunye imeju n'ụwa niile.

A na-ebute HCV site na yakọntaktị ọbara na-ọbara, na ụzọ mbufe a na-ahụkarị gụnyere:

Ịkekọrịta agịga ma ọ bụ sirinji emetọ, karịsịa n'iji ọgwụ eme ihe n'ime eriri afọ.
mmịnye ọbarama ọ bụ ntụgharị akụkụ ahụ site n'aka ndị na-enye onyinye na-enweghị nyocha (ọ bụ ezie na ọ dị ụkọ n'ihi nyocha siri ike).
Mmekọahụ mmekọahụ na-enweghị nchebe(obere obere).
Site na nne bu oria rue nwan'oge ọmụmụ nwa (perinatal nnyefe).

N'adịghị ka ọrịa imeju ndị ọzọ,A naghị agbasa ọrịa HCV site na nri ma ọ bụ mmiri.

Nchọpụta mbụ nkeHCVdị oke mkpa maka njikwa dị irè, n'ihi na ọrịa ahụ nwere ike ịbụ asymptomatic ruo ọtụtụ afọ, na-enye ohere maka ọganihu na-adịghị ahụ anya nke mmebi imeju.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa:

Mmetụta dị elu na nkọwapụta
Ezubere iji chọpụta nke ọmaọgwụ mgbochi HCV, na-enye nsonaazụ a pụrụ ịdabere na ya na obere ihe ize ndụ nke ụgha ma ọ bụ ihe ọjọọ.
Nsonaazụ ngwa ngwa
Nnwale ahụ na-enye nsonaazụ n'ime15-20 nkeji, na-akwado mkpebi n'oge banyere nlekọta ndị ọrịa na nlekọta nlekọta.
Ọ dị mfe iji
Nnwale a dị mfe iji nye ya na-enweghị mkpa maka ọzụzụ ma ọ bụ akụrụngwa pụrụ iche, na-eme ka ọ dị mma maka ojiji na ntọala ahụike dị iche iche.
Ụdị Nlereanya Na-agbanwe agbanwe
Nnwale na-arụ ọrụọbara dum, ọbara, ma ọ bụplasma, na-enye mgbanwe na mkpokọta sample.
Enwere ike ibugharị ma dịkwa mma maka iji ubi
Nhazi kọmpat na nke dị arọ nke ngwa ule na-eme ka ọ dị mmangalaba ahụike mkpanaka, mmemme mgbasa ozi obodo, namkpọsa ahụike ọha.

Ụkpụrụ:

NkeNgwa HCV ngwa ngwana-arụ ọrụ dabere naimmunochromatography(teknụzụ na-erugharị n'akụkụ) ịchọpụtaọgwụ mgbochi nje ịba ọcha n'anya C (anti-HCV)na sample. Usoro a gụnyere usoro ndị a:

Ihe atụ mgbakwunye
A na-agbakwunye ntakịrị ọbara zuru oke, serum, ma ọ bụ plasma n'ime olulu mmiri nlele nke ngwaọrụ ahụ, yana ngwọta nchekwa.
Mmeghachi omume Antigen-Antibody
Kaseti ule nwere recombinantHCV antigensnke na-enweghị ike imegharị na ahịrị ule. Ọ bụrụọgwụ mgbochi HCVdị na nlele ahụ, ha ga-ejikọta na antigens ma mepụta mgbagwoju anya antigen-antibody.
Mbugharị chromatographic
Ihe mgbagwoju anya antigen-antibody na-akwaga n'akụkụ akpụkpọ ahụ site na ọrụ capillary. Ọ bụrụ na ọgwụ mgbochi HCV dị, ha ga-ejikọta n'ahịrị ule (T line), na-emepụta eriri agba a na-ahụ anya. Reagents ndị fọdụrụ ga-akwaga na ahịrị njikwa (C line) iji gosi na ule ahụ arụwo ọrụ nke ọma.
Nkọwapụta nsonaazụ
- Ahịrị abụọ (akara T + C):Nsonaazụ dị mma, na-egosi ọnụnọ nke mgbochi HCV.
- Otu ahịrị (akara C naanị):Nsonaazụ na-adịghị mma, na-egosi enweghị ọgwụ mgbochi HCV a na-achọpụta.
- Enweghị ahịrị ma ọ bụ T naanị:Nsonaazụ ezighi ezi, chọrọ nnwale ugboro ugboro.

Ihe mejupụtara:

Ihe mejupụtara	Ọnụ ego	Nkọwapụta
IFU	1	/
Nwalee cassette	25	Akpa foil ọ bụla emechiri emechi nwere otu ngwaọrụ nnwale na otu ihe eji eme ihe
mmịpụta diluent	500μL * 1 Tube * 25	Ihe nchekwa Tris-Cl, NaCl, NP 40, ProClin 300
Ntụpọ dropper	25	/
Swab	/	/

Usoro nnwale:


1. Saa aka gi	2. Lelee ihe dị n'ime ngwa ahụ tupu ịnwale, gụnyere ntinye ngwugwu, kaseti ule, ihe nchekwa, swab.	3. Tinye tube mmịpụta na ebe a na-arụ ọrụ.	4.Kpechapụ ihe mkpuchi aluminom si n'elu nke ntanye mmịpụta nke nwere ihe ntinye ihe.

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5. Jiri nlezianya wepụ swab na-emetụghị ọnụ ọnụ. Tinye ọnụ ọnụ nke swab 2 ruo 3 cm n'ime imi imi ziri ezi. Rịba ama ebe mgbawa nke imi imi. Ị nwere ike inwe mmetụta nke a na mkpịsị aka gị mgbe ị na-etinye imi imi ma ọ bụ lelee. ya n'isi. Na-ete ime ime imi na mmegharị okirikiri ugboro 5 maka opekata mpe 15 sekọnd, Ugbu a were otu imi imi ma tinye ya n'oghere imi ọzọ. Biko jiri ihe nlele were mee nnwale ahụ ozugbo ma emela ya
hapụ ya ka ọ kwụ.

6. Tinye swab na tube mmịpụta. Gbanwee swab maka ihe dị ka 10 sekọnd, gbanwee swab megide tube nke na-emepụta ihe, na-agbanye isi nke swab megide n'ime tube ahụ ka ị na-agbanye akụkụ nke tube ahụ iji hapụ dị ka mmiri mmiri. dị ka o kwere mee site na swab.


7. Wepụ swab na ngwugwu na-emetụghị ihe mgbochi ahụ aka.	8.Mix nke ọma site n'ịgbanye ala nke tube ahụ. Tinye 3 tụlee nke nlele ahụ n'ụzọ ziri ezi n'ime ihe nlele nke ọma nke cassette ule. Gụọ ihe si na ya pụta mgbe minit 15 gasịrị. Rịba ama: Gụọ nsonaazụ n'ime nkeji 20. Ma ọ bụghị ya, a na-akwado arịrịọ maka ule.

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