SARS-CoV-2 Neutralizing antibody Test Cassette

Maka ntule qualitative nke Ọrịa Coronavirus 2019 (2019-nCOV ma ọ bụ COVID-19) na-ewepụ ihe mgbochi n'ime ọbara mmadụ / plasma / ọbara dum.

Maka ọkachamara In Vitro Diagnostic Jiri naanị

【E zubere iji】

Ihe nyocha nke SARS-CoV-2 Neutralizing antibody Test Cassette bụ chromatographic ngwa ngwa

immunoassay maka nchọpụta qualitative nke neutralizing mgbochi nke ọrịa Coronavirus 2019 n'ime ọbara mmadụ dum, serum, ma ọ bụ plasma dị ka ihe enyemaka na ọkwa nyocha nke mmadụ mgbochi akwụkwọ akụkọ coronavirus na-ewepụ ihe mgbochi.

Mammals.The genus γ tumadi na-akpata nnụnụ ọrịa.CoV na-bụ tumadi ebu site kpọmkwem kọntaktị na secretions ma ọ bụ site aerosols na ụmụ irighiri mmiri. E nwekwara ihe àmà na-egosi na a pụrụ ibunye ya site n'ụzọ fecal-oral.

Ọrịa iku ume siri ike nke coronavirus 2 (SARS-CoV-2, ma ọ bụ 2019-nCoV) bụ nje RNA na-enweghị akụkụ nke ọma. Ọ bụ ihe kpatara ọrịa coronavirus 2019 (COVID-19), nke na-efe efe n'ime mmadụ.

SARS-CoV-2 nwere ọtụtụ protein arụpụtara gụnyere spike (S), envelopu (E), akpụkpọ ahụ (M) na nucleocapsid (N). Protein spike (S) nwere ngalaba nnabata nnabata (RBD), nke na-ahụ maka ịmata onye na-anabata sel elu, angiotensin na-agbanwe enzyme-2 (ACE2). Achọpụtara na RBD nke protein SARS-CoV-2 S na-emekọrịta ihe na onye na-anabata ACE2 mmadụ na-eduga na endocytosis n'ime sel nnabata nke ngụgụ miri emi na mmegharị nje.

Ọrịa SARS-CoV-2 na-ebute nzaghachi mgbochi ọrịa, nke gụnyere mmepụta nke ọgwụ mgbochi ọrịa n'ọbara. Ọgwụ nje ndị a zoro ezo na-enye nchebe pụọ na ọrịa nje n'ọdịnihu, n'ihi na ha na-anọ n'ime usoro ọbara ruo ọnwa ole na ole mgbe ọrịa ahụ gasịrị, ha ga-ejikọta ngwa ngwa na ike na pathogen iji gbochie ntinye cellular na mmegharị. A na-akpọ ọgwụ mgbochi ndị a na-eme ihe mgbochi neutralizing.

【 Mkpokọta na Nkwadebe ihe atụ】

1. Ezubere kaseti nnwale SARS-CoV-2 Neutralizing antibody bụ maka iji naanị ọbara mmadụ, serum ma ọ bụ plasma zuru oke.

2.Only doro anya, ndị na-abụghị hemolyzed specimens ka akwadoro maka ojiji na ule a. Ekwesịrị ịkewa serum ma ọ bụ plasma ozugbo enwere ike iji zere hemolysis.

3.Mee ule ozugbo nchịkọta ihe atụ. Ahapụla ihe nlere anya na ụlọ okpomọkụ ruo ogologo oge. Enwere ike ịchekwa ihe nlele ọbara na plasma na 2-8 Celsius ruo ụbọchị 3. Maka nchekwa ogologo oge, a ga-edobe ihe nrịbama serum ma ọ bụ plasma n'okpuru-20 Celsius C. A ga-echekwa ọbara niile nke venipuncture na-anakọta na 2-8 Celsius C ma ọ bụrụ na a ga-eme ule ahụ n'ime ụbọchị 2 mgbe nchịkọtachara. Akwụsịla ọbara zuru ezu. ụdị. Ekwesịrị ịnwale ọbara niile anakọtara site na mkpịsị aka ozugbo.

4.Containers nwere anticoagulants dị ka EDTA, citrate, ma ọ bụ heparin kwesịrị iji maka nchekwa ọbara dum.Weta ihe atụ na okpomọkụ ụlọ tupu nyocha.

5.Frozen specimens ga-kpamkpam gbazee ma gwakọta nke ọma tupu ule. Zere kefriza ugboro ugboro.

na thawing nke ụdị.

6.Ọ bụrụ na a ga-ebupu ihe nlere anya, bukọta ha na nrube isi na iwu niile dị maka njem.

nke etiological agents.

7.Icteric,lipemic,hemolyzed,okpomọkụ mesoo na mmeru sera nwere ike ịkpata na-ezighị ezi pụta.

8.Mgbe ị na-anakọta ọbara mkpịsị aka na lancet na mmanya mmanya,biko tụfuo mgbụsị akwụkwọ mbụ.

ọbara dum.

1. Weta obere akpa ahụ n'ime ụlọ okpomọkụ tupu imeghe. Wepụ ngwaọrụ nyocha na obere akpa ahụ mechiri emechi ma jiri ya ozugbo enwere ike.

2. Debe ngwaọrụ ule na elu dị ọcha na larịị.

Maka nyocha ọbara ma ọ bụ Plasma: Iji Micropipette, ma bufee 5ul serum/plasma n'ụdị nke ọma nke ngwaọrụ nnwale, tinyezie 2 dobe nchekwa, wee malite ngụ oge.

Maka Ọbara Nile (Venipuncture/Fingerstick) Ụdị: Kpoo mkpịsị aka gị ma jiri nwayọọ pịpịa mkpịsị aka gị, jiri pipette rọba nke a na-atụfu na-enye ka ọ ṅụọ 10ul nke ọbara dum na eriri 10ul nke pipette plastik a na-ekpofu, ma bufee ya na oghere ihe atụ nke ngwaọrụ ule (Ọ bụrụ na ọnụ ọgụgụ ọbara dum karịrị akara ahụ, Biko hapụ ọbara zuru oke na pipette), wee tinye 2 dobe nke nchekwa, ma malite ngụ oge. Mara: Enwere ike itinye ihe nlele site na iji micropipette.

3. Chere ka ahịrị (s) na-acha acha pụta. Gụpụta nsonaazụ na nkeji 15. Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara.