Ngwa SARS-CoV-2 Na-anọpụ iche na-egbochi mmadụ (ELISA)
【Ezubere iji】
SARS-CoV-2 Neutralizing Antibody Detection Kit bụ Asọmpi Enzyme-Linked Immunosorbent Assay (ELISA) ezubere maka nchọpụta qualitative na ọkara nke mkpokọta mgbochi mgbochi SARS-CoV-2 na ọbara mmadụ na plasma. Enwere ike iji SARS-CoV-2 Neutralizing Antibody Detection Kit dị ka ihe enyemaka n'ịchọpụta ndị mmadụ nwere nzaghachi mgbochi mgbochi na SARS-CoV-2, na-egosi ọrịa na-adịbeghị anya ma ọ bụ bu ụzọ. E kwesịghị iji ngwa nchọpụta SARS-CoV-2 Neutralizing Antibody chọpụta ọrịa SARS-CoV-2 siri ike.
【Okwu mmalite】
Ọrịa Coronavirus na-ebute nzaghachi mgbochi mmadụ. Ọnụego mgbanwe seroconversion na ndị ọrịa COVID-19 bụ 50% na 100% n'ụbọchị 7 na 14 ka akara mmalite malitere, n'otu n'otu. Iji gosipụta ihe ọmụma, nje na-egbochi nje na-egbochi nje n'ọbara bụ nke a ghọtara dị ka ebumnuche maka ịchọpụta nrụpụta mgbochi mmadụ yana itinye uche dị elu nke mgbochi mgbochi na-egosi nrụpụta nchekwa dị elu. Achọpụtala Nnwale Mbelata Neutralization Plaque (PRNT) dị ka ọkọlọtọ ọla edo maka ịchọpụta mgbochi mgbochi. Agbanyeghị, n'ihi ntinye ya dị ala yana ihe achọrọ dị elu maka ịrụ ọrụ, PRNT adịghị arụ ọrụ maka nnukwu serodiagnosis na nyocha ọgwụ mgbochi. Ngwa SARS-CoV-2 Neutralizing Antibody Detection Kit dabere na usoro asọmpi Enzyme-Linked Immunosorbent Assay (ELISA), nke nwere ike ịchọpụta mgbochi na-ewepụ n'ọbara ọbara yana nweta ọkwa ịta ahụhụ nke ụdị mgbochi a.
【Usoro ule】
1.In dị iche iche tubes, aliquot 120μL nke njikere hACE2-HRP Ngwọta.
2.Tinye 6 μL nke calibrators, ihe ndị a na-amaghị, njikwa mma na tube ọ bụla ma jikọta nke ọma.
3. Nyefee 100μL nke ngwakọta ọ bụla a kwadebere na nzọụkwụ 2 n'ime olulu mmiri microplate kwekọrọ dị ka nhazi ule a kpọpụtara.
3. Na-ekpuchi efere ahụ na Plate Sealer na incubate na 37 ° C maka nkeji 60.
4.Wepụ Plate Sealer ma saa efere ahụ na ihe dịka 300 μL nke 1 × Wash Solution kwa nke ọma maka ugboro anọ.
5. Kpatụ efere na akwa nhicha akwụkwọ iji wepụ mmiri mmiri fọdụrụ na olulu mmiri mgbe ịsachara nzọụkwụ.
6.Tinye 100 μL nke TMB Ngwọta na nke ọ bụla ma tinye efere na ọchịchịrị na 20 - 25 ° C maka nkeji 20.
7.Tinye 50 μL nke Ngwọta Kwụsị na olulu mmiri ọ bụla iji kwụsị mmeghachi omume.
8.Gụọ ihe ntinye na onye na-agụ microplate na 450 nm n'ime nkeji 10 (630nm dị ka ngwa na-atụ aro maka ịrụ ọrụ nkenke dị elu.
