Otu nzọụkwụ SARS-CoV2 (COVID-19) IgG/IgM Test
Ezubere iji
Otu nzọụkwụ SARS-CoV2 (COVID-19) IgG/IgM Test bụ ngwa ngwa chromatographic immunoassay maka nchọpụta qualitative nke ọgwụ mgbochi ọrịa (IgG na IgM) ka nje COVID-19 n'ime ọbara dum / Serum / Plasma iji nyere aka na nyocha nke COVID -19 ọrịa nje.
Nchịkọta
Nje virus Corona bụ nje RNA kpuchiri ekpuchi nke na-ekesa n'etiti ụmụ mmadụ, anụmanụ ndị ọzọ na nnụnụ na nke na-ebute ọrịa iku ume, enteric, hepatic and neurologic.A mara ụdị nje corona asaa na-ebute ọrịa mmadụ.Nje virus anọ-229E.OC43.NL63 na HKu1- na-ewu ewu ma na-ebutekarị mgbaàmà oyi na-ahụkarị na ndị mmadụ na-ahụ maka ọrịa ahụ. 19)- bụ zoonotic sitere na nke ejirila ya na ọrịa na-egbu egbu mgbe ụfọdụ.Enwere ike ịchọpụta ọgwụ mgbochi IgG na lgM na 2019 Novel Coronavirus na izu 2-3 ka ekpughere ya.lgG ka dị mma, mana ọkwa mgbochi na-agbadata oge.
Ụkpụrụ
Otu nzọụkwụ SARS-CoV2 (COVID-19) IgG/IgM (Ọbara dum / Serum / Plasma) bụ nyocha nke immunochromatographic n'akụkụ.Nnwale a na-eji mgbochi mmadụ lgM antibody (ule ule IgM), mgbochi mmadụ lgG(ule ule lgG na ewu mgbochi rabbit igG (akara njikwa C) nke na-adịghị emegharị na nitrocellulose strip. COVID-19 antigens jikọtara ya na colloid gold (COVID-19 conjugate na oke bekee lgG-ọlaedo conjugates. Mgbe agbakwunyere ihe nlere na-esote nke assay buffer na sample nke ọma, IgM &/ma ọ bụ lgG ọgwụ mgbochi ọ bụrụ na ọ dị, ga-ejikọta na COVID-19 conjugates na-eme. Mgbagwoju anya antigen na-akwaga site na nitrocellulose membrane site na capillary edinam mgbe mgbagwoju ahụ zutere ahịrị nke mgbochi mmadụ (mgbochi mmadụ IgM &/ma ọ bụ anit-human lgG) mgbagwoju ahụ na-akpụ akpụ acha agba agba. Nsonaazụ nleba anya nke enweghị agba agba na mpaghara ule na-egosi nsonaazụ ule anaghị arụ ọrụ.
Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba agba nke immunocomplex ewu anti rabbit IgG/oke bekee lgG-gold conjugate n'agbanyeghị mmepe agba na ụdọ ule ọ bụla.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.
Nchekwa na kwụsie ike
- Chekwaa dị ka akpachịkọtara n'akpa mkpuchi mkpuchi na ụlọ okpomọkụ ma ọ bụ friji (4-30 ℃ ma ọ bụ 40-86 ℉).Ngwaọrụ nnwale ahụ kwụsiri ike site na ụbọchị njedebe ebipụtara na obere akpa ahụ emechiri emechi.
- Nnwale ahụ ga-adịrịrị n'ime obere akpa ahụ emechiri emechi ruo mgbe ejiri ya.
Ngwa Pụrụ Iche Ndị Ọzọ
Ihe ndị enyere:
| .Nnwale ngwaọrụ | .droppers ụdị atụfu |
| .Ihe nchekwa | .Ntinye ngwugwu |
Ihe achọrọ mana enyeghị ya:
| .Centrifuge | .Ngụ oge |
| .Ihe mgbochi mmanya | .Akpa nchịkọta ihe atụ |
Mkpachapụ anya
☆ Maka ọkachamara in vitro diagnostic were naanị.Ejila ụbọchị ngafe gachara.
☆ Erila ihe, ṅụọ ihe ọṅụṅụ ma ọ bụ sịga n'ebe a na-edozi ihe nlere na ngwa.
☆ Jikwaa ụdịdị niile dị ka a ga-asị na ha nwere ihe na-efe efe.
☆ Debe nlezianya anya megide ihe egwu microbiological n'ime usoro niile ma soro usoro ọkọlọtọ maka mkpofu nke ọma.
☆ Yiri uwe nchebe dị ka uwe ụlọ nyocha, uwe mkpuchi a na-atụfu n'anya na nchebe anya mgbe a na-enyocha ihe nlele.
☆ Soro ntuziaka bio-safety ọkọlọtọ maka njikwa na mkpofu ihe nwere ike ibute ọrịa.
☆ Iru mmiri na okpomọkụ nwere ike imetụta nsonaazụ.
Nchịkọta na Nkwadebe ihe atụ
1. Enwere ike ịme ule SARS-CoV2 (COVID-19) IgG/IgM na Ọbara dum / Serum / Plasma.
2. Iji nakọta ọbara zuru oke, serum ma ọ bụ plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.
3. Ekwesịrị ime nnwale ozugbo ị nwetachara ihe atụ.Ahapụla ihe nlere anya na ụlọ okpomọkụ ruo ogologo oge.Maka nchekwa ogologo oge, a ga-edobe ụdị ihe n'okpuru -20 ℃.Ekwesịrị ịchekwa ọbara niile na 2-8 ℃ ma ọ bụrụ na a ga-eme nnwale ahụ n'ime ụbọchị 2 nke nchịkọta.Akwụsịla ụdị ọbara dum.
4. Weta ụdị n'ime ụlọ okpomọkụ tupu anwale.Ụdị oyi kpọnwụrụ ga-agbaze kpamkpam ma gwakọta ya nke ọma tupu anwale ya.Agaghị ajụ oyi ma gbazee ihe atụ ugboro ugboro.
Usoro ule
1. Kwe ka ule, ụdị, ihe nchekwa na/ma ọ bụ njikwa iru ụlọ okpomọkụ 15-30℃ (59-86℉) tupu ule.
2. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya.Wepu ngwaọrụ nnwale ahụ na obere akpa mkpuchi ma jiri ya ozugbo enwere ike.
3. Debe ngwaọrụ ule na elu dị ọcha na larịị.
4. Jide dropper kwụ ọtọ wee bufee 1 dobe nke ụdị (ihe dị ka 10μl) na nke ọma (S) nke ngwaọrụ ule, wee tinye 2 tụlee nke ihe nchekwa (ihe dị ka 70μl) wee malite ngụ oge.Lee ihe atụ n'okpuru.
5. Chere ka ahịrị(s) nwere agba pụta.Gụọ nsonaazụ na nkeji iri na ise.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.
ndetu:
Itinye ihe nlere zuru oke dị mkpa maka nsonaazụ ule dị mma.Ọ bụrụ na ahụghị ọpụpụ (wetting nke akpụkpọ ahụ) na mpio ule mgbe otu nkeji gachara, tinye otu dobe nchekwa ọzọ na ihe nlele ahụ nke ọma.
Nkọwa nke nsonaazụ
Nke dị mma:Ahịrị njikwa na opekata mpe otu ahịrị nnwale na-apụta na akpụkpọ ahụ.Mpụta nke ahịrị ule T2 na-egosi ọnụnọ nke COVID-19 kpọmkwem ọgwụ mgbochi IgG.Ọdịdị nke ahịrị ule T1 na-egosi ọnụnọ nke COVID-19 kpọmkwem ọgwụ mgbochi IgM.Ma ọ bụrụ na ahịrị T1 na T2 pụtara, ọ na-egosi na ọnụnọ nke ma COVID-19 kpọmkwem IgG na IgM mgbochi.Na ala nke mgbochi antibody bụ, na-esighị ike n'ihi na ahịrị.
adịghị mma:Otu ahịrị agba na-egosi na mpaghara njikwa (C) .Ọ nweghị ahịrị agba pụtara na mpaghara ahịrị ule.
Na ezighi ezi:Ahịrị njikwa agaghị apụta.Oke ihe atụ ezughi oke ma ọ bụ usoro usoro na-ezighi ezi bụ ihe kachasị kpatara ọdịda akara njikwa.Nyochaa usoro ahụ wee jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.Ọ bụrụ na nsogbu ahụ dịgidere, kwụsị iji ngwa nnwale ozugbo wee kpọtụrụ onye nkesa mpaghara gị.
Oke
1.Nnwale SARS-CoV2 (COVID-19) IgG/IgM bụ naanị iji nyocha nyocha in vitro.Ekwesịrị iji ule ahụ mee nchọpụta ihe mgbochi COVID-19 n'ime ọbara dum / Serum / Plasma naanị.Ma uru ọnụọgụ ma ọ bụ ọnụego mmụba na 2. COVID-19 ọgwụ mgbochi ọrịa nwere ike ikpebi site na ule qualitative a.
3. Dị ka ọ dị na nyocha nyocha niile, a ga-atụgharịrịrị nsonaazụ niile yana ozi ụlọ ọgwụ ndị ọzọ dị na dọkịta.
4. Ọ bụrụ na nsonaazụ nyocha ahụ dị njọ na mgbaàmà ụlọ ọgwụ na-adịgide adịgide, a na-atụ aro nyocha ọzọ site na iji usoro ọgwụgwọ ndị ọzọ.Nsonaazụ na-adịghị mma anaghị egbochi oge ọ bụla enwere ike ibute ọrịa COVID-19.
Ozi ngosi
Asambodo nsọpụrụ
Nkọwapụta Ụlọ ọrụ
Anyị, Hangzhou Testsea Biotechnology Co., Ltd bụ ụlọ ọrụ ọkachamara biotechnology na-eto ngwa ngwa ọkachamara na nyocha, mmepe, nrụpụta na nkesa nke ngwa ule in-vitro diagnostics (IVD) dị elu na ngwa ahụike.
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Usoro ngwaahịa
1. Kwadebe
2.Mkpuchi
3.Cross akpụkpọ ahụ
4.Ibe warara
5.Mgbakọ
6. Kpochie obere akpa
7.Achie akpa ahụ
8.Pack igbe
9. Ihe mkpuchi
