Ngwa nyocha SARS-CoV-2 ezigbo oge RT-PCR

Ezubere ngwa a maka nchọpụta qualitative in vitro nke ORF1ab na N mkpụrụ ndụ sitere na 2019-nCoV na pharyngeal swab ma ọ bụ bronchoalveolar lavage ụdị anakọtara site na ọrịa Coronavirus 2019 (COVID-19), ndị a na-enyo enyo, ụyọkọ nke a na-enyo enyo, ma ọ bụ ndị ọzọ chọrọ 2019. - nCoV ọrịa nchoputa ma ọ bụ iche iche nchọpụta.

 onyonyo002

Emebere ngwa a maka nchọpụta RNA nke 2019-nCoV n'ụdị na-eji teknụzụ RTPCR multiplex ezigbo oge yana yana mpaghara echekwara ORF1ab na mkpụrụ ndụ N dị ka saịtị ebumnuche nke primers na nyocha.N'otu oge ahụ, ngwa a nwere usoro nchọpụta njikwa endogenous (Genen njikwa akara bụ Cy5) iji nyochaa usoro nchịkọta ihe atụ, mmịpụta nucleic acid na PCR ma belata nsonaazụ ọjọọ ụgha.

 onyonyo004

Akụkụ ndị bụ isi:

1. Ngwa ngwa, nkwalite ntụkwasị obi na nchọpụta inclusivity: SARS dị ka coronavirus na nchọpụta kpọmkwem nke SARS-CoV-2

2. Otu nzọụkwụ RT-PCR reagent (lyophilized ntụ ntụ)

3. Na-agụnye njikwa dị mma na nke na-adịghị mma

4. Ụgbọ njem na okpomọkụ nkịtị

5. Ngwa ahụ nwere ike ịnọgide na-akwụsi ike ruo ọnwa 18 echekwara na -20 ℃.

6. CE kwadoro

Na-asọ:

1. Kwadebe RNA ewepụtara na SARS-CoV-2

2. Ghichaa njikwa RNA dị mma na mmiri

3. Kwadebe PCR master mix

4. Tinye PCR master mix na RNA n'ime ezigbo PCR efere ma ọ bụ tube

5. Gbaa ngwa PCR ozugbo

 onyonyo006


Oge nzipu: Nov-09-2020

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