Ngwa nyocha SARS-CoV-2 ezigbo oge RT-PCR

Ezubere ngwa a maka nchọpụta qualitative in vitro nke ORF1ab na N mkpụrụ ndụ sitere na 2019-nCoV na pharyngeal swab ma ọ bụ bronchoalveolar lavage ụdị anakọtara site na ọrịa Coronavirus 2019 (COVID-19), ndị a na-enyo enyo, ụyọkọ nke a na-enyo enyo, ma ọ bụ ndị ọzọ chọrọ 2019. - nCoV ọrịa nchoputa ma ọ bụ iche iche nchọpụta.

Emebere ngwa ahụ maka nchọpụta RNA nke 2019-nCoV n'ụdị na-eji teknụzụ RTPCR multiplex ezigbo oge yana yana mpaghara echekwara ORF1ab na mkpụrụ ndụ N dị ka saịtị ebumnuche nke primers na nyocha. N'otu oge, ngwa a nwere usoro nchọpụta njikwa endogenous (Genen njikwa akara bụ Cy5) iji nyochaa usoro nchịkọta ihe atụ, mmịpụta nucleic acid na PCR ma belata nsonaazụ ọjọọ ụgha.

Akụkụ ndị bụ isi:

1. Ngwa ngwa, nkwalite ntụkwasị obi na nchọpụta inclusivity: SARS dị ka coronavirus na nchọpụta kpọmkwem nke SARS-CoV-2

2. Otu nzọụkwụ RT-PCR reagent (lyophilized ntụ ntụ)

3. Na-agụnye njikwa dị mma na nke na-adịghị mma

4. Ụgbọ njem na okpomọkụ nkịtị

5. Ngwa ahụ nwere ike ịnọgide na-akwụsi ike ruo ọnwa 18 echekwara na -20 ℃.

6. CE kwadoro

Na-asọ:

1. Kwadebe RNA ewepụtara na SARS-CoV-2

2. Ghichaa njikwa RNA dị mma na mmiri

3. Kwadebe PCR master mix

4. Tinye PCR master mix na RNA n'ime ezigbo PCR efere ma ọ bụ tube

5. Gbaa ngwa PCR ozugbo