Ngwa ule ICH-CPV-CDV IgG

A haziri CANINE INFECTIOUS HEEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ICH/CPV/CDV IgG test kit) iji nyochaa obere ihe mgbochi nkịta IgG maka nje virus Canine Infectious Hepatitis (ICH), Canine Parvo Virus (CPV) na Nje Virus Canine Distemper (CDV).

Ọdịnaya ngwa

Nhazi na ụkpụrụ

Enwere ihe abụọ etinyere na katrij nke ọ bụla: Igodo, nke etinyere ya na desiccant na mpaghara ala nke ejiri mkpuchi aluminom na-echebe, yana ihe ngwọta na-emepụta, nke a na-edobere iche n'ime ụlọ elu nke ejiri ihe mkpuchi aluminum kpuchie.

Katrij ọ bụla nwere reagents niile dị mkpa maka otu nlele nlele. Na nkenke, mgbe etinyere igodo ahụ n'ime ma tinye ya maka nkeji ole na ole n'ime akụkụ elu nke 1, bụ ebe a na-edebe ihe nlele ọbara, ihe mgbochi IgG kpọmkwem dị n'ụdị ọbara agbazere, ma ọ bụrụ na ọ dị, ga-ejikọta na ICH, CPV ma ọ bụ CDV recombinant antigens anaghị emegharị n'ebe dị iche iche dị iche iche na igodo etinyere. Mgbe ahụ, a ga-ebufe mkpịsị ugodi ahụ n'ime ụlọ ndị fọdụrụ n'elu n'oge oge na-aga site na nzọụkwụ. A ga-edobe ọgwụ mgbochi IgG akọwapụtara na ntụpọ ahụ na ngalaba 3 dị elu, nke nwere mgbochi canine IgG enzyme conjugate na nsonaazụ ikpeazụ ewepụtara dị ka ntụpọ na-acha anụnụ anụnụ na igodo ga-emepụta n'elu.

nkebi 6, nke nwere mkpụrụ. Maka nsonaazụ na-eju afọ, ewebata usoro ịsa ahụ. N'ime akụkụ 2 dị elu, a ga-ewepụ IgG enweghị oke na ihe ndị ọzọ dị n'ime nlele ọbara. N'elu akụkụ 4 na 5, ndị na-enweghị oke ma ọ bụ

Mgbochi mgbochi canine IgG enzyme conjugate ga-ekpochapụ nke ọma. Na njedebe, n'ime akụkụ 7 nke elu, a ga-ewepụ chromosome ngafe nke sitere na mkpụrụ na enzyme conjugate jikọtara ọnụ na ngalaba 6 nke elu. Iji gosi izi ezi nke arụmọrụ, a na-ewebata protein njikwa n'elu ebe kachasị na igodo ahụ. Ekwesịrị ịhụ ntụpọ na agba odo odo-acha anụnụ anụnụ ka emechara usoro nnwale na-aga nke ọma.

Nchekwa

1. Chekwaa kit n'okpuru refrigeration nkịtị (2 ~ 8 ℃).

AKWỤKWỌ KWESỊRỊ ỤWA.

2. Ngwa ahụ nwere ihe ndụ anaghị arụ ọrụ. Ekwesịrị ijikwa ngwa ahụ

ma tụfuo ya dị ka ihe chọrọ ịdị ọcha nke mpaghara.

Usoro ule

Nkwadebe tupu emee ule:

1. Weta katrij na ụlọ okpomọkụ (20 ℃-30 ℃) ma tinye ya na bench ọrụ ruo mgbe akara thermal na mgbidi nke katrij na-aghọ uhie uhie.

2. Tinye akwụkwọ anụ ahụ dị ọcha n'elu oche ọrụ maka itinye igodo ahụ.

3. Kwadebe ihe nkesa 10μL na 10μL ọkọlọtọ pipette ndụmọdụ.

4. Wepu foil aluminom na-echebe ala wee tụpụ igodo ahụ n'ime akụkụ ala nke katrij na akwụkwọ anụ ahụ dị ọcha.

5. Guzo ọtọ katrij na oche ọrụ ma gosi na ọnụ ọgụgụ nke elu ụlọ nwere ike ịhụ na ntụziaka ziri ezi (stampụ nọmba ziri ezi chere gị ihu). Kpatụ katrijị ahụ ntakịrị ka ị hụ na ihe ngwọta ndị dị n'elu ọnụ ụlọ na-atụgharị azụ na ala.

Na-eme ule:

1.Mepee foil nchebe n'elu akụkụ nke ọma nke ọma na mkpịsị aka na mkpịsị aka site n'aka ekpe gaa n'aka nri ruo mgbe nanị ikpughe akụkụ elu 1.

2.Nweta ihe nlele ọbara a nwalere na ihe nkesa ihe na-eji ọkọlọtọ 10μL pipette.

Maka nnwale ọbara ma ọ bụ plasma, jiri 5 μl.

Maka ule ọbara dum, jiri 10μL.

A na-atụ aro EDTA ma ọ bụ heparin tubes anticoagulant maka plasma na mkpokọta ọbara dum.

3. Deposit sample n'ime n'elu ngalaba 1. Mgbe ahụ, welie na ala dispenser plunger ọtụtụ ugboro iji nweta Ịgwakọta (Light-acha anụnụ anụnụ ngwọta na n'ọnụ mgbe agwakọta na-egosi ihe ịga nke ọma sample nkwụnye ego).

4. Were mkpịsị aka mkpịsị aka na mkpịsị aka were were were nlezianya were mkpịsị ugodi were tinye igodo ahụ n'ime akụkụ nke elu 1 (gosipụta akụkụ oyi nke igodo chere gị ihu, ma ọ bụ gosi na ọkara okirikiri dị n'aka nri mgbe chere ihu. ị). Mgbe ahụ gwakọta ma guzoro igodo dị n'elu ọnụ ụlọ 1 maka nkeji ise.

5. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe naanị ikpughe akụkụ ahụ 2. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 2. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 2 maka nkeji 1.

6. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe naanị ikpughe akụkụ ahụ 3. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 3. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'ime akụkụ 3 maka nkeji ise.

7.Mepee foil nchebe na-aga n'ihu n'aka nri ruo mgbe naanị ikpughe akụkụ 4. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 4. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 4 maka nkeji 1.

8. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe nanị ikpughe akụkụ 5. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 5. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 5 maka nkeji 1.

9. Mepee foil nchebe na-aga n'ihu ruo n'aka nri ruo mgbe nanị ikpughe akụkụ 6. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 6. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 6 maka nkeji ise.

10. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe nanị ikpughe akụkụ 7. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 7. Mgbe ahụ gwakọta ma guzoro igodo dị n'elu 7 maka nkeji 1.

11. Wepụ igodo ahụ n'elu nkebi 7 ma hapụ ya ka ọ kpọọ nkụ na akwụkwọ anụ ahụ maka ihe dịka nkeji 5 tupu ị gụọ nsonaazụ.

ndetu:

Emetụla akụkụ Frosting nke ihu njedebe nke igodo, ebe antigens na protein njikwa na-enweghị ike imegharị (Nleba Nnwale na Njikwa).

Zere ịcha mpaghara Ule na njikwa site na ịdabere n'akụkụ ọzọ dị nro nke ihu ihu igodo na mgbidi dị n'ime nke ọnụ ụlọ ọ bụla n'elu mgbe ị na-agwakọta.

Maka ịgwakọta, a na-atụ aro ibuli na iwetu igodo ugboro 10 n'akụkụ nke ọ bụla n'elu.

Naanị kpughee otu akụkụ dị elu na-esote tupu ibufee igodo ahụ.

Ọ bụrụ na ọ dị mkpa, tinye akara ngosi Pet nyere maka ihe karịrị otu nlele nlele.

Ntughari ule

Jiri ọkọlọtọ ColorScale lelee ntụpọ arụpụtara na igodo ahụ

Na ezighi ezi:

Ọ BỤGHỊ agba odo odo-acha anụnụ anụnụ a na-ahụ anya na-egosi na ebe njikwa

Ihe ọjọọ(-)

Ọ BỤGHỊ agba odo odo-acha anụnụ anụnụ a na-ahụ anya na-egosi na ntụpọ ule

Nke dị mma (+)

Agba odo-acha anụnụ anụnụ a na-ahụ anya na-egosi n'ebe ule

Enwere ike iji ọkwa atọ gosipụta titter nke ọgwụ mgbochi IgG akọwapụtara