Ngwa ule FPLVFHVFCV IgG

Emebere FELINE PANLEUKOPENIA/HERPES VIRUS/CALICI VIRUS IgG ANTIBODY TEST KIT (FPLV/FHV/FCV IgG test kit) iji nyochaa ọkara nke pusi IgG antibody maka Feline Panleukopenia (FPLV), Feline Herpes Virus (FHV) na Feline Herpes Virus (FHV). Nje Virus (FCV).

Ọdịnaya ngwa

Nhazi na ụkpụrụ

Enwere ihe abụọ etinyere na katrij nke ọ bụla: Igodo, nke etinyere ya na desiccant na mpaghara ala nke ejiri mkpuchi aluminom na-echebe, yana ihe ngwọta na-emepụta, nke a na-edobere iche n'ime ụlọ elu nke ejiri ihe mkpuchi aluminum kpuchie. Katrij ọ bụla nwere reagents niile dị mkpa maka otu nlele nlele. Na nkenke, mgbe etinyere igodo ahụ n'ime ma tinye ya maka nkeji ole na ole n'ime akụkụ elu nke 1, bụ ebe a na-edebe ihe nlele ọbara, ihe mgbochi IgG kpọmkwem dị n'ime ọbara agbazere, ma ọ bụrụ na ọ dị, ga-ejikọta na FPLV, FHV ma ọ bụ FCV recombinant antigens anaghị emegharị na iche iche

ntụpọ pụrụ iche na igodo etinyere. Mgbe ahụ, a ga-ebufe mkpịsị ugodi ahụ n'ime ụlọ ndị fọdụrụ n'elu n'oge oge na-aga site na nzọụkwụ. A ga-edobe ọgwụ mgbochi IgG akọwapụtara na ntụpọ ahụ na ngalaba 3 dị elu, nke nwere mgbochi feline IgG enzyme conjugate na nsonaazụ ikpeazụ ewepụtara dị ka ntụpọ odo odo na-acha anụnụ anụnụ na ngalaba 6 nke nwere mkpụrụ. Maka nsonaazụ na-eju afọ, ewebata usoro ịsa ahụ. N'ime akụkụ 2 dị elu, a ga-ewepụ IgG enweghị oke na ihe ndị ọzọ dị n'ime nlele ọbara. N'ime akụkụ 4 na 5 dị elu, a ga-ekpochapụ ihe mgbochi feline IgG enzyme conjugate enweghị oke ma ọ bụ karịa. Na njedebe, n'ime akụkụ 7 nke elu, a ga-ewepụ chromosome ngafe nke sitere na mkpụrụ na enzyme conjugate jikọtara ọnụ na ngalaba 6 nke elu.

Iji gosi izi ezi nke arụmọrụ, a na-ewebata protein njikwa n'elu ebe kachasị na igodo ahụ. Agba odo odo-acha anụnụ anụnụ kwesịrị ịhụ anya mgbe emechara usoro nyocha nke ọma.

Nchekwa

1. Chekwaa kit n'okpuru refrigeration nkịtị (2 ~ 8 ℃).

AKWỤKWỌ KWESỊRỊ ỤWA.

2. Ngwa ahụ nwere ihe ndụ anaghị arụ ọrụ. Ekwesịrị ijikwa ngwa ahụ

ma tụfuo ya dị ka ihe chọrọ ịdị ọcha nke mpaghara.

Usoro ule

Nkwadebe tupu emee ule:

1. Weta katrij na ụlọ okpomọkụ (20 ℃-30 ℃) ma tinye ya na bench ọrụ ruo mgbe akara thermal na mgbidi nke katrij na-aghọ uhie uhie.

2. Tinye akwụkwọ anụ ahụ dị ọcha n'elu oche ọrụ maka itinye igodo ahụ.

3. Kwadebe ihe nkesa 10μL na 10μL ọkọlọtọ pipette ndụmọdụ.

4. Wepu foil aluminom na-echebe ala wee tụpụ igodo ahụ n'ime akụkụ ala nke katrij na akwụkwọ anụ ahụ dị ọcha.

5. Guzo ọtọ katrij na oche ọrụ ma gosi na ọnụ ọgụgụ nke elu ụlọ nwere ike ịhụ na ntụziaka ziri ezi (stampụ nọmba ziri ezi chere gị ihu). Kpatụ katrijị ntakịrị ka ị hụ na ọ dị

ngwọta n'ime akụkụ ndị dị elu na-atụgharị azụ na ala.

Na-eme ule:

1.Mepee foil nchebe n'elu ọnụ ụlọ nke ọma jiri mkpịsị aka ekpe na mkpịsị aka ekpe gaa n'aka nri ruo mgbe naanị ikpughe akụkụ elu 1.

2.Nweta ihe nlele ọbara a nwalere na ihe nkesa ihe na-eji ọkọlọtọ 10μL pipette.

Maka nnwale ọbara ma ọ bụ plasma, jiri 5 μl.

Maka ule ọbara dum, jiri 10μL.

A na-atụ aro EDTA ma ọ bụ heparin tubes anticoagulant maka plasma na mkpokọta ọbara dum.

3. Deposit sample n'ime n'elu ngalaba 1. Mgbe ahụ, welie na ala dispenser plunger ọtụtụ ugboro iji nweta Ịgwakọta (Light-acha anụnụ anụnụ ngwọta na n'ọnụ mgbe agwakọta na-egosi ihe ịga nke ọma sample nkwụnye ego).

4. Were mkpịsị aka mkpịsị aka na mkpịsị aka were were were nlezianya were mkpịsị ugodi were tinye igodo ahụ n'ime akụkụ nke elu 1 (gosipụta akụkụ oyi nke igodo chere gị ihu, ma ọ bụ gosi na ọkara okirikiri dị n'aka nri mgbe chere ihu. ị). Mgbe ahụ gwakọta ma guzoro igodo dị n'elu ọnụ ụlọ 1 maka nkeji ise.

5. Kpuchie foil nchebe na-aga n'ihu ruo n'aka nri ruo mgbe naanị ikpughe akụkụ ahụ 2. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 2. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'ime oghere ahụ.

elu ụlọ 2 maka 1 nkeji.

6. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe naanị ikpughe akụkụ ahụ 3. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 3. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'ime oghere ahụ.

nkeji 3 maka nkeji 5.

7.Mepee foil nchebe na-aga n'ihu n'aka nri ruo mgbe naanị ikpughe akụkụ 4. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 4. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 4 maka nkeji 1.

8. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe nanị ikpughe akụkụ 5. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 5. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 5 maka nkeji 1.

9. Mepee foil nchebe na-aga n'ihu ruo n'aka nri ruo mgbe nanị ikpughe akụkụ 6. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 6. Mgbe ahụ gwakọta ma guzoro igodo ahụ n'elu akụkụ 6 maka nkeji ise.

10. Kpuchie foil nchebe na-aga n'ihu n'aka nri ruo mgbe nanị ikpughe akụkụ 7. Were igodo ahụ site na njide ma tinye igodo ahụ n'ime oghere ekpughere 7. Mgbe ahụ gwakọta ma guzoro igodo dị n'elu 7 maka nkeji 1.

11. Wepụ igodo ahụ n'elu nkebi 7 ma hapụ ya ka ọ kpọọ nkụ na akwụkwọ anụ ahụ maka ihe dịka nkeji 5 tupu ị gụọ nsonaazụ.

 ndetu:

Emetụla akụkụ Frosting nke ihu njedebe nke igodo, ebe antigens na protein njikwa na-enweghị ike imegharị (Nleba Nnwale na Njikwa).

Zere ịcha mpaghara Ule na njikwa site na ịdabere n'akụkụ ọzọ dị nro nke ihu ihu igodo na mgbidi dị n'ime nke ọnụ ụlọ ọ bụla n'elu mgbe ị na-agwakọta.

Maka ịgwakọta, a na-atụ aro ibuli na iwetu igodo ugboro 10 n'akụkụ nke ọ bụla n'elu.

Naanị kpughee otu akụkụ dị elu na-esote tupu ibufee igodo ahụ.

Ọ bụrụ na ọ dị mkpa, tinye akara ngosi Pet nyere maka ihe karịrị otu nlele nlele.

Ntughari ule

Jiri ọkọlọtọ ColorScale lelee ntụpọ arụpụtara na igodo ahụ

Na ezighi ezi:

Ọ BỤGHỊ agba odo odo-acha anụnụ anụnụ a na-ahụ anya na-egosi na ebe njikwa

Ihe ọjọọ(-)

Ọ BỤGHỊ agba odo odo-acha anụnụ anụnụ a na-ahụ anya na-egosi na ntụpọ ule

Nke dị mma (+)

Agba odo-acha anụnụ anụnụ a na-ahụ anya na-egosi n'ebe ule

Enwere ike iji ọkwa atọ gosipụta titter nke ọgwụ mgbochi IgG akọwapụtara