SARS-COV-2 nruab nrab tshuaj tiv thaiv antibldy nrhiav cov khoom siv (Elisa)
【Siv】
Sars-COV-1 neutraling antiblody nrhiav cov khoom siv yog cov tshuaj tua kab mob sib tw nrog SARS-CE-2 nyob rau hauv tib neeg thiab ntshav. Qhov SARS- CE-2 nruab nrab tshuaj tiv thaiv tshuaj tiv thaiv cov khoom siv tau tuaj yeem siv los ntawm kev txheeb xyuas tus neeg muaj mob tiv thaiv kab mob, qhia txog kev kis tus kab mob tsis ntev los no. SARS-COV-2 Meshrationalizing tshuaj tiv thaiv tshuaj kho cov khoom siv yuav tsum tsis txhob siv los kuaj mob sars-txoj kab-1 tus kab mob.
【Lub hauv paus rau phau ntawv】
Cov kab mob Coronavirus ib txwm ua rau cov lus teb tsis zoo nruab nrab. Cov Seroconversion tus nqi hauv kevid-19% yog 50% thiab 100% rau hnub 7 thiab 14 post cov tsos mob pib, feem. Txhawm rau nthuav tawm cov txuj ci, tus kab mob sib xws nruab nrab hauv cov ntshav tau lees paub cov tshuaj tiv thaiv kab mob antiberny thiab ntau dua cov tshuaj tiv thaiv antibuty qhia kev ua kom muaj txiaj ntsig ntau dua. Kev ntsuas ntshav txo kev puas tsuaj (PrnT) tau raug lees paub tias yog tus qauv kub rau kev tshawb pom cov tshuaj tiv thaiv nruab nrab. Txawm li cas los xij, vim nws qhov qes qis thiab siab dua rau qhov ua haujlwm, prny tsis yog cov tswv yim rau cov nplai loj serodiagosxisis thiab kev tshuaj tiv thaiv kab mob. SARS-COV-2 Meshrational ua tshuaj tiv thaiv tshuaj tiv thaiv kab mob yog ua kom pom kev sib tw ntawm cov ntshav ntxiv rau cov ntshav ntawm hom tshuaj tiv thaiv no.
【Cov txheej txheem ntsuas】
1. Tsoo cais cov raj, aliquot 120μl ntawm npaj HACE2-HRP daws.
2.Add 6 μl ntawm cov ntsuas kub, cov qauv tsis paub, tswj tau zoo hauv txhua lub raj thiab sib tov zoo.
3.Qhov 100μ2 ntawm txhua qhov sib xyaw npaj hauv qib 2 mus rau hauv cov qauv microplate sib piv raws li kev sim ua ntej.
3.Cov phiaj rau phaj nrog phaj seoomering thiab ncos ntawm 37 ° C rau 60 feeb.
4.Qhov lub phaj foob thiab ntxuav cov phaj nrog kwv yees 300 μl ntawm 1 × ntxuav kev daws teeb meem rau plaub zaug.
5.Taap lub phaj rau ntawm daim ntawv phuam kom tshem cov kua dej uas seem hauv cov hmoov tom qab ntxuav cov kauj ruam.
6.Ntadd 100 μl ntawm tmb daws rau txhua qhov dej thiab ua kom tiav cov phaj tsaus ntuj ntawm 20 - 25 ° C rau 20 feeb.
7.DD 50 μTxhua kev daws teeb meem rau txhua qhov zoo kom nres qhov tshuaj tiv thaiv.
8.Nyuaj kev nqus tau nyob rau hauv micppate nyeem ntawv ntawm 450 nm hauv 10 feeb (630nm li accessory yog pom zoo rau cov kev ua tau zoo dua.
