SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)
【YUAV TSUM SIV】
SARS-CoV-2 Neutralizing Antibody Detection Kit yog Competitive Enzyme-Linked Immunosorbent Assay (ELISA) npaj rau kev kuaj pom zoo thiab ib nrab ntawm tag nrho cov tshuaj tiv thaiv tsis zoo rau SARS-CoV-2 hauv tib neeg cov ntshav thiab ntshav. SARS- CoV-2 Neutralizing Antibody Detection Kit tuaj yeem siv los ua ib qho kev pabcuam hauv kev txheeb xyuas cov tib neeg uas muaj kev hloov kho lub cev tiv thaiv kab mob rau SARS- CoV-2, qhia txog kev kis mob tsis ntev los no lossis yav dhau los. SARS-CoV-2 Neutralizing Antibody Detection Kit yuav tsum tsis txhob siv los kuaj mob SARS-CoV-2 mob hnyav.
【Taw qhia】
Cov kab mob Coronavirus feem ntau ua rau muaj kev cuam tshuam cov tshuaj tiv thaiv kab mob. Cov kev hloov pauv hloov pauv hauv cov neeg mob COVID-19 yog 50% thiab 100% nyob rau hnub 7 thiab 14 tom qab cov tsos mob tshwm sim, feem. Txhawm rau nthuav tawm cov kev paub, cov kab mob sib kis nruab nrab ntawm cov tshuaj tiv thaiv kab mob hauv cov ntshav yog qhov lees paub tias yog lub hom phiaj rau kev txiav txim siab cov tshuaj tiv thaiv kab mob thiab cov concentration siab dua ntawm cov tshuaj tiv thaiv tsis zoo qhia tau tias muaj kev tiv thaiv ntau dua. Plaque Reduction Neutralization Test (PRNT) tau raug lees paub tias yog tus qauv kub rau kev kuaj xyuas cov tshuaj tiv thaiv kab mob. Txawm li cas los xij, vim nws qhov kev xa tawm tsawg thiab kev xav tau ntau dua rau kev ua haujlwm, PRNT tsis yog qhov ua tau zoo rau kev kuaj mob loj thiab tshuaj tiv thaiv. SARS-CoV-2 Neutralizing Antibody Detection Kit yog raws li Competitive Enzyme-Linked Immunosorbent Assay (ELISA) txoj kev, uas tuaj yeem ntes cov tshuaj tiv thaiv tsis zoo hauv cov ntshav thiab tshwj xeeb nkag mus rau qib siab ntawm hom tshuaj tiv thaiv no.
【Cov txheej txheem xeem】
1.In cais cov raj, aliquot 120μL ntawm cov tshuaj npaj hACE2-HRP.
2.Ntxiv 6 μL ntawm calibrators, tsis paub cov qauv, kev tswj zoo hauv txhua lub raj thiab sib tov zoo.
3.Hloov 100μL ntawm txhua qhov sib tov npaj rau hauv cov kauj ruam 2 mus rau hauv cov dej microplate sib xws raws li kev sim ua ntej kev teeb tsa.
3. Npog lub phaj nrog Plate Sealer thiab incubate ntawm 37 ° C rau 60 feeb.
4.Tshem lub Phaj Sealer thiab ntxuav lub phaj nrog kwv yees li 300 μL ntawm 1 × Ntxuav Tshuaj rau plaub zaug.
5. Coj mus rhaub lub phaj ntawm daim ntawv phuam kom tshem tawm cov kua seem hauv qhov dej tom qab ntxuav cov kauj ruam.
6.Ntxiv 100 μL ntawm TMB Tshuaj rau txhua qhov dej thiab ua rau lub phaj hauv qhov tsaus ntuj ntawm 20 - 25 ° C rau 20 feeb.
7.Ntxiv 50 μL ntawm Stop Solution rau txhua qhov dej kom tsis txhob muaj cov tshuaj tiv thaiv.
8.Nyeem cov absorbance hauv microplate nyeem ntawv ntawm 450 nm hauv 10 feeb (630nm raws li cov khoom siv tau pom zoo rau kev ua haujlwm siab dua.
