SARS-CoV-2 Real-time RT-PCR Detection Kit

Cov khoom siv no yog npaj rau hauv vitro qualitative detection ntawm ORF1ab thiab N noob los ntawm 2019-nCoV nyob rau hauv pharyngeal swab los yog bronchoalveolar lavage specimens sau los ntawm tus kab mob Coronavirus 2019 (COVID-19) cov neeg mob, xav tias muaj pawg neeg mob, lossis lwm tus neeg uas xav tau 2019. - nCoV infection diagnosis or differentiation diagnosis.

Cov khoom siv yog tsim los rau RNA nrhiav pom ntawm 2019-nCoV hauv cov qauv siv multiplex real time RTPCR thev naus laus zis thiab nrog thaj chaw khaws cia ntawm ORF1ab thiab N noob raws li lub hom phiaj ntawm cov primers thiab probes. Tib lub sijhawm, cov khoom siv no muaj cov txheej txheem tswj endogenous (Cov noob tswj tau sau los ntawm Cy5) los saib xyuas cov txheej txheem ntawm kev sau cov qauv, nucleic acid extraction thiab PCR thiab txo cov txiaj ntsig tsis zoo.

Cov yam ntxwv tseem ceeb:

1. Ceev ceev, txhim khu kev qha amplification thiab kev tshawb nrhiav suav nrog: SARS zoo li tus kabmob coronavirus thiab kev kuaj pom tshwj xeeb ntawm SARS-CoV-2

2. Ib-kauj ruam RT-PCR reagent (lyophilized hmoov)

3. suav nrog kev tswj zoo thiab tsis zoo

4. Kev thauj mus los ntawm qhov kub thiab txias

5. Cov khoom siv tuaj yeem ruaj khov mus txog 18 lub hlis khaws cia ntawm -20 ℃.

6. CE pom zoo

Flow:

1. Npaj rho tawm RNA los ntawm SARS-CoV-2

2. Dilute zoo tswj RNA nrog dej

3. Npaj PCR master mix

4. Siv PCR master mix thiab RNA rau hauv lub sijhawm tiag tiag PCR phaj lossis raj

5. Khiav lub cuab yeej PCR lub sijhawm