ʻO SARS-CoV-2 Hoʻopaʻa ʻana i ka Antibody Detection Kit （ELISA)

【KA MANAO ANA】

ʻO SARS-CoV-2 Neutralizing Antibody Detection Kit he Competitive Enzyme-Linked Immunosorbent Assay (ELISA) i manaʻo ʻia no ka qualitative a me ka semi-quantitative detection o ka huina neutralizing antibodies i SARS-CoV-2 i ka serum kanaka a me ka plasma. Hiki ke hoʻohana ʻia ka SARS- CoV-2 Neutralizing Antibody Detection Kit i mea kōkua i ka ʻike ʻana i nā poʻe me kahi pane kūlohelohe adaptive iā SARS- CoV-2, e hōʻike ana i ka maʻi hou a i ʻole ma mua. ʻAʻole pono e hoʻohana ʻia ka SARS-CoV-2 Neutralizing Antibody Detection Kit e ʻike i ka maʻi maʻi SARS-CoV-2.

【OLELO HOOLAHA】

ʻO nā maʻi maʻi Coronavirus e hoʻoulu pinepine i nā pane antibody. ʻO ka helu seroconversion i nā maʻi maʻi COVID-19 he 50% a me 100% ma ka lā 7 a me 14 ma hope o ka hoʻomaka ʻana o ka hōʻailona. No ka hōʻike ʻana i ka ʻike, ʻike ʻia ka maʻi virus neutralizing antibody i loko o ke koko ma ke ʻano he pahuhopu no ka hoʻoholo ʻana i ka pono o ka antibody a ʻoi aku ka kiʻekiʻe o ka neʻe ʻana o ka antibody e hōʻike ana i ka ʻoi aku ka maikaʻi o ka pale. Ua ʻike ʻia ka Plaque Reduction Neutralization Test (PRNT) i ke kūlana gula no ka ʻike ʻana i nā antibodies neutralizing. Eia nō naʻe, ma muli o kona haʻahaʻa haʻahaʻa a me ke koi kiʻekiʻe no ka hana, ʻaʻole kūpono ʻo PRNT no ka serodiagnosis nui a me ka loiloi kano. Hoʻokumu ʻia ka SARS-CoV-2 Neutralizing Antibody Detection Kit ma ka Competitive Enzyme-Linked Immunosorbent Assay (ELISA) methodology, hiki ke ʻike i ka antibody neutralizing i loko o ke koko koko a me ka loaʻa ʻana o nā pae koʻikoʻi o kēia ʻano antibody.

 【Kaʻina hana hoʻāʻo】

1.Ma nā paipu ʻokoʻa, aliquot 120μL o ka HACE2-HRP Solution i hoʻomākaukau ʻia.

2.E hoʻohui i 6 μL o nā calibrators, nā mea iʻikeʻoleʻia, nā mana maikaʻi i kēlā me kēia paipu a hui maikaʻi.

3. E hoʻololi i 100μL o kēlā me kēia hui ʻana i hoʻomākaukau ʻia ma ka ʻanuʻu 2 i loko o nā pūnāwai microplate e like me ka hoʻonohonoho hoʻāʻo predesigned.

3. E uhi i ka pā me ka Plate Sealer a hoʻoulu i ka 37 ° C no 60 mau minuke.

4. Wehe i ka Plate Sealer a holoi i ka pā me ka 300 μL o 1 × Wash Solution i kēlā me kēia punawai no ʻehā mau manawa.

5. E kāomi i ka pā ma ke kāwele pepa e wehe i ke koena wai i loko o nā pūnāwai ma hope o ka holoi ʻana.

6. E hoʻohui i ka 100 μL o ka TMB Solution i kēlā me kēia punawai a hoʻomoʻa i ka pā i ka pōʻeleʻele ma 20 - 25 ° C no 20 mau minuke.

7. E hoʻohui i 50 μL o Stop Solution i kēlā me kēia pūnāwai e hoʻopau i ka hopena.

8. E heluhelu i ka absorbance i ka microplate heluhelu ma 450 nm i loko o 10 minuke (630nm e like me ka mea kōkua no ka oi precision hana.