Hoʻolālā ʻia kēia pahu no ka ʻike in vitro qualitative o ORF1ab a me N genes mai ka 2019-nCoV i loko o ka pharyngeal swab a i ʻole bronchoalveolar lavage specimens i hōʻiliʻili ʻia mai ka maʻi Coronavirus 2019 (COVID-19) i manaʻo ʻia i nā hihia, nā puʻupuʻu i manaʻo ʻia o nā hihia, a i ʻole nā kānaka ʻē aʻe e pono ai 2019 - ka maʻi maʻi nCoV a i ʻole ka ʻike ʻokoʻa.
Hoʻolālā ʻia ka pahu no ka ʻike ʻana o RNA o 2019-nCoV i nā specimens me ka hoʻohana ʻana i ka ʻenehana RTPCR mau manawa maoli a me nā wahi i mālama ʻia o ORF1ab a me N genes ma ke ʻano he wahi i kuhikuhi ʻia o nā primers a me nā probes. I ka manawa like, loaʻa i kēia pahu kahi ʻōnaehana ʻike mana endogenous (Ua kapa ʻia ka mana mana e Cy5) e nānā i ke kaʻina hana o ka hōʻiliʻili specimen, ka ʻokiʻoki nucleic acid a me ka PCR a hōʻemi i nā hopena maikaʻi ʻole.
Nā mea nui:
1. Hoʻonui wikiwiki, hilinaʻi a me ka ʻike ʻana: ʻo SARS e like me ka coronavirus a me ka ʻike kikoʻī o SARS-CoV-2
2. Hoʻokahi ʻanuʻu RT-PCR reagent (lyophilized pauka)
3. Loaʻa nā mana maikaʻi a maikaʻi ʻole
4. ʻO ka halihali i ka mahana maʻamau
5. Hiki ke paʻa i ka pahu a hiki i 18 mahina i mālama ʻia ma -20 ℃.
6. Aponoia CE
Kahe:
1. E hoʻomākaukau i ka RNA i unuhi ʻia mai SARS-CoV-2
2. E hoʻoheheʻe i ka RNA mana maikaʻi me ka wai
3. E hoʻomākaukau i ka PCR master mix
4. E hoʻopili i ka PCR master mix a me ka RNA i loko o ka pā PCR manawa maoli
5. Holo i kahi mea hana PCR manawa maoli
Ka manawa kau: Nov-09-2020