Mea hoʻomaʻi ʻo Monkeypox (MPV) pahu ʻike ʻakika nucleic
OLELO HOOLAHA
Hoʻohana ʻia ka pahu no ka ʻike in vitro qualitative o nā hihia i manaʻo ʻia o ka Monkeypox Virus (MPV), nā hihia i hui pū ʻia a me nā hihia ʻē aʻe e pono e ʻike ʻia no ka maʻi maʻi Monkeypox.
Hoʻohana ʻia ka pahu no ka ʻike ʻana i ka gene f3L o ka MPV ma nā ʻāʻī ʻāʻī a me nā laʻana nasal swab.
ʻO nā hopena hoʻāʻo o kēia kit no ka hōʻike lapaʻau wale nō a ʻaʻole pono e hoʻohana ʻia ma ke ʻano he hōʻailona hoʻokahi no ka maʻi lapaʻau. Manaʻo ʻia e hana i kahi loiloi piha o ka maʻi e pili ana i ke kauka o ka mea maʻi
hōʻike a me nā hoʻokolohua ʻē aʻe.
Hoohana Manao
| ʻAno hoʻāʻo | ʻauʻau ʻāʻī a me ka pulu ihu |
| ʻAno hoʻāʻo | Kūlana |
| Mea ho'āʻo | PCR |
| Nui pūʻolo | 48 ho'āʻo/1 pahu |
| Ka mahana mālama | 2-30 ℃ |
| Ola ola | 10 mahina |
HIʻO HUA
Kumukānāwai
Lawe kēia pahu i ke kaʻina i mālama pono ʻia o ka gene MPV f3L ma ke ʻano he wahi i hoʻopaʻa ʻia. Hoʻohana ʻia ka ʻenehana PCR fluorescence quantitative maoli a me ka ʻenehana hoʻokuʻu wikiwiki i ka nucleic acid e nānā i ka waikawa nucleic viral ma o ka hoʻololi ʻana o ka hōʻailona fluorescence o nā huahana amplification. Aia ka ʻōnaehana ʻike i ka mana maikaʻi o loko, i hoʻohana ʻia e nānā inā he PCR inhibitors i loko o nā laʻana a i ʻole nā pūnaewele i loko o nā laʻana, hiki ke pale pono i ke kūlana maikaʻi ʻole.
NA MEA NUI
Aia i loko o ka pahu nā reagents no ka hoʻoponopono ʻana i nā hoʻokolohua 48 a i ʻole ka mana maikaʻi, me nā ʻāpana aʻe:
ʻO Reagent A
| inoa | Nā mea nui | Ka nui |
| ʻIke MPV reagent | Aia ka Mg2+ i loko o ka paipu hopena, f3L gene / Rnase P primer probe, hoʻopaʻa pane, Taq DNA enzyme. | 48 hoao |
ReagentB
| inoa | Nā mea nui | Ka nui |
| MPV Manaʻo maikaʻi | Loaʻa i kahi ʻāpana target MPV | 1 paipu |
| MPV Manaʻo ʻino | Me ka ʻole o ka ʻāpana pahuhopu MPV | 1 paipu |
| DNA hoʻokuʻu reagent | Aia i loko o ka reagent ʻo Tris, EDTA a me Triton. | 48 pcs |
| Mea hoʻoponopono hou | ʻO ka wai i mālama ʻia e DEPC | 5ML |
'Ōlelo Aʻo: ʻAʻole hiki ke hoʻohana ʻia nā ʻāpana o nā helu pūʻulu like ʻole
【Nā Kūlana Hoʻopaʻa a me ke Ola Paʻa】
1.Reagent A / B hiki ke mālamaʻia ma 2-30 ° C, a he 10 mahina ke ola.
2.Eʻoluʻolu e wehe i ka uhi pahu ho'āʻo wale nō ke mākaukauʻoe no ka ho'āʻo.
3. Mai hoʻohana i nā paipu ho'āʻo ma mua o ka lā pau.
4. Mai hoʻohana i kahi paipu ʻike leaking.
【Mea Hana Pono】
He kūpono no ka LC480 PCR system analysis, Gentier 48E Automatic PCR analysis system, ABI7500 PCR analysis system.
【Nā Koina Laʻana】
1.Applicable laʻanaʻano: puʻu ʻāʻī nā laʻana.
2. Sampling solution:Ma hope o ka hōʻoia ʻana, ʻōlelo ʻia e hoʻohana i ka saline maʻamau a i ʻole ka pahu mālama mālama Virus i hana ʻia e Hangzhou Testsea biology no ka hōʻiliʻili laʻana.
puʻu ʻāʻī:e holoi i nā tonsils pharyngeal bilateral a me ka paia pharyngeal hope me ka swab sterile sampling, e hoʻokomo i ka swab i loko o ka paipu me 3mL sampling solution, e hoʻolei i ka huelo, a hoʻopaʻa i ka uhi o ka paipu.
3.Sample waihona a me ka lawe ana:Pono e hoʻāʻo koke ʻia nā laʻana e hoʻāʻo ʻia. Pono e mālama ʻia ka mahana o ka halihali ma 2 ~ 8 ℃. Hiki ke mālama ʻia nā laʻana i hiki ke hoʻāʻo ʻia i loko o 24 mau hola ma 2 ℃ ~ 8 ℃ a inā ʻaʻole hiki ke hoʻāʻo ʻia nā mea hoʻohālike i loko o 24 mau hola, pono e mālama ʻia ma lalo a i ʻole like. i -70 ℃ (inā ʻaʻohe kūlana mālama o -70 ℃, hiki ke mālama ʻia ma -20 ℃ no ka manawa pōkole), pale i ka hana hou
ka hau a me ka hau.
4. ʻO ka hōʻiliʻili hōʻiliʻili kūpono, ka mālama ʻana, a me ka lawe ʻana he mea koʻikoʻi i ka hana o kēia huahana.
【ʻAno hoʻāʻo】
1.Sample hana a me ka hoʻohui hāpana
1.1 Hana laʻana
Ma hope o ka hui ʻana i ka hopena laʻana i luna me nā laʻana, e lawe i 30μL o ka hāpana i loko o ka pahu hoʻokuʻu DNA reagent a hui like.
1.2 Hoʻouka
E lawe i 20μL o ka reagent reconstitution a hoʻohui iā ia i ka MPV detection reagent, e hoʻohui i 5μL o ka laʻana i hana ʻia ma luna (E hana ʻia ka mana maikaʻi a me ka mana maikaʻi ʻole e like me nā laʻana), uhi i ka pāpale paipu, centrifuge ia ma 2000rpm no 10 kekona.
2. Hoʻonui PCR
2.1 E hoʻouka i ka pā PCR i hoʻomākaukau ʻia i ka mea hana PCR fluorescence.
2.2 Ka hoʻonohonoho ʻana i ke kahawai Fluorescent:
1) E koho i ke ala FAM no ka ʻike MPV;
2) E koho i ke kaila HEX/VIC no ka ʻike ʻana i ke ʻano o loko;
3.Nānā hopena
E hoʻonoho i ka laina kumu ma luna o ka piko kiʻekiʻe loa o ka ʻōkuhi fluorescent o ka mana ʻino.
4.Quality mana
4.1 Manaʻo ʻino: ʻAʻohe waiwai Ct i ʻike ʻia ma FAM, HEX/VIC channel, a i ʻole Ct＞40；
4.2 Mana maikaʻi: Ma FAM, HEX/VIC channel, Ct≤40；
4.3 Pono e hoʻokō ʻia nā koi i luna ma ka hoʻokolohua hoʻokahi, inā ʻaʻole pono nā hopena hōʻike a pono e hana hou ʻia ka hoʻokolohua.
【ʻOki i ka waiwai】
Manaʻo ʻia kahi laʻana i ka maikaʻi inā: ʻO ke kaʻina pahuhopu Ct≤40, ʻO ka gene mana kūloko Ct≤40.
【ʻO ka wehewehe ʻana i nā hopena】
Ke hala ka mana maikaʻi, pono nā mea hoʻohana e nānā inā aia kahi pihi hoʻonui no kēlā me kēia laʻana ma ke kahawai HEX/VIC, inā aia a me Ct≤40, ua hōʻike ʻia ua hoʻonui maikaʻi ʻia ka gene mana kūloko a ua kūpono kēia hōʻike. Hiki i nā mea hoʻohana ke hoʻomau i ka nānā ʻana:
3. No nā laʻana me ka hoʻonui ʻana i ka gene mana kūloko i hāʻule (HEX/VIC
Kanal, Ct＞40, a i ʻole ʻaʻohe kaha hoʻonui), haʻahaʻa Viral haʻahaʻa a i ʻole ke ola ʻana o ka PCR inhibitor ke kumu o ka hāʻule ʻole, pono e hana hou ka hoʻokolokolo mai ka hōʻiliʻili specimen;
4. No nā hōʻailona maikaʻi a me ka moʻomeheu moʻomeheu, ʻaʻole pili nā hopena o ka mana kūloko;
No nā laʻana i hoʻāʻo maikaʻi ʻole ʻia, pono e hoʻāʻo maikaʻi ʻia ka mana kūloko i ʻole he kūpono ʻole ka hopena holoʻokoʻa a pono e hana hou ʻia ka hoʻokolokolo ʻana, e hoʻomaka ana mai ka pae hōʻiliʻili specimen.
ʻIke Hōʻikeʻike
Palapala Hoʻohanohano
ʻIkepili Hui
ʻO mākou, ʻo Hangzhou Testsea Biotechnology Co., Ltd he hui ʻoihana biotechnology e ulu wikiwiki ana i ka noiʻi, hoʻomohala, hana a me ka hāʻawi ʻana i nā pahu hoʻāʻo in-vitro diagnostic (IVD) a me nā mea lapaʻau.
ʻO kā mākou hale ʻo GMP, ISO9001, a me ISO13458 i hōʻoia ʻia a loaʻa iā mākou ka ʻae ʻana o CE FDA. I kēia manawa ke kakali nei mākou i ka launa pū ʻana me nā ʻoihana ʻē aʻe no ka hoʻomohala ʻana.
Hoʻopuka mākou i ka hoʻāʻo fertility, nā hoʻāʻo ʻana i nā maʻi infectious, nā hoʻāʻo hoʻohana ʻana i nā lāʻau lapaʻau, nā hōʻailona hōʻailona naʻau, nā hōʻailona hōʻailona tumora, nā hoʻāʻo ʻana i ka meaʻai a me ka palekana a me nā hoʻāʻo ʻana i nā maʻi holoholona. ʻO ka maikaʻi maikaʻi a me nā kumukūʻai maikaʻi e hiki ai iā mākou ke lawe ma luna o 50% o nā ʻāpana kūloko.
Kaʻina Huahana
1. Hoʻomākaukau
2.Ka uhi
3. Cross membrane
4.ʻoki ʻoki
5.Ahui
6. Hoʻopili i nā ʻeke
7. E sila i nā ʻeke
8. Hoʻopili i ka pahu
9.Encasement
Kāohi i kahi pōʻino hou: E hoʻomākaukau i kēia manawa i ka laha ʻana o ka Monkeypox
Ma ʻAukake 14th, ua hoʻolaha ka World Health Organization (WHO) i ka puka ʻana o ka monkeypox he "Public Health Emergency of International Concern." ʻO kēia ka lua o ka manawa i hoʻopuka ai ʻo WHO i ka pae kiʻekiʻe o ka makaʻala e pili ana i ka maʻi maʻi monkeypox mai Iulai 2022.
I kēia manawa, ua hoʻolaha ʻia ka maʻi monkeypox mai ʻApelika a i ʻEulopa a me ʻAsia, me nā hihia i hōʻoia ʻia ma Sweden a me Pakistan.
Wahi a ka ʻikepili hou loa mai ʻApelika CDC, i kēia makahiki, ua hōʻike ʻia he 12 mau lālā o ka Hui ʻApelika i ka huina o 18,737 mau hihia monkeypox, me 3,101 mau hihia i hoʻopaʻa ʻia, 15,636 mau hihia i manaʻo ʻia, a me 541 mau make, me ka helu o ka make o 2.89%.
01 He aha ka Monkeypox?
ʻO ka Monkeypox (MPX) kahi maʻi zoonotic viral i hoʻokumu ʻia e ka maʻi monkeypox. Hiki ke hoʻoili ʻia mai nā holoholona i nā kānaka, a ma waena o nā kānaka. ʻO nā hōʻailona maʻamau e pili ana i ke kuni, ka ʻeha, a me ka lymphadenopathy.
Komo mua ka ma'i monkeypox i ke kino o ke kanaka ma o ka mucous membrane a me ka ili haki. ʻO nā kumu o ka maʻi e pili ana i nā maʻi monkeypox a me nā rodents i hoʻopili ʻia, nā monkey, a me nā primate ʻaʻole kanaka. Ma hope o ka maʻi, ʻo ka manawa incubation he 5 a 21 mau lā, maʻamau 6 a 13 mau lā.
ʻOiai ua maʻalahi ka heluna kanaka i ka maʻi maʻi monkeypox, aia kekahi ʻano o ka pale ʻana i ka monkeypox no ka poʻe i hoʻopaʻa ʻia i ka maʻi maʻi liʻiliʻi, ma muli o ke ʻano genetic a me ka antigenic like ma waena o nā maʻi. I kēia manawa, laha nui ka monkeypox i waena o nā kāne e moe ana me nā kāne ma o ka hoʻopaʻa ʻana, ʻoiai ke emi nei ka pilikia o ka maʻi o ka lehulehu.
02 Pehea ka ʻokoʻa o kēia maʻi maʻi maʻi maʻi?
Mai ka hoʻomaka ʻana o ka makahiki, ʻo ka maʻi nui o ka maʻi monkeypox, "Clade II," ua hoʻokumu i kahi maʻi maʻi nui a puni ka honua. ʻO ka mea hopohopo, ke piʻi aʻe nei ka nui o nā hihia i hoʻokumu ʻia e "Clade I," ʻoi aku ka koʻikoʻi a ʻoi aku ka nui o ka make, a ua hōʻoia ʻia ma waho o ka ʻāina ʻApelika. Eia kekahi, mai ka mahina o Kepakemapa i ka makahiki i hala, he ʻano hou, ʻoi aku ka make a me ka maʻalahi hiki ke hoʻololi ʻia, "Clade Ib," ua hoʻomaka e laha ma ka Demokarata Repubalika o Congo.
ʻO kahi hiʻohiʻona kaulana o kēia maʻi maʻi, ʻo nā wahine a me nā keiki ma lalo o 15 mau makahiki ka mea i hoʻopilikia nui ʻia.
Hōʻike ka ʻikepili ma luna o 70% o nā hihia i hōʻike ʻia i nā maʻi ma lalo o 15 mau makahiki, a ma waena o nā hihia make, piʻi kēia helu i 85%. ʻIke ʻia,ʻO ka nui o ka make no nā keiki he ʻehā manawa kiʻekiʻe ma mua o nā pākeke.
03 He aha ka pilikia o ka lawe ʻana i ka Monkeypox?
Ma muli o ka manawa mākaʻikaʻi a me ka launa pū ʻana o ka honua, hiki ke piʻi aʻe ka pilikia o ka hoʻoili ʻia ʻana o ka maʻi monkeypox. Eia nō naʻe, laha nui ka maʻi ma o ka pilina pili lōʻihi, e like me ka moe kolohe, ka ʻili ʻili, a me ka hanu kokoke ʻana a i ʻole ke kamaʻilio ʻana me nā poʻe ʻē aʻe, no laila ua nāwaliwali kona hiki ke hoʻouna i ke kanaka.
04 Pehea e pale aku ai i ka maʻi ʻino?
E hōʻalo i ka launa pū ʻana me ka poʻe i ʻike ʻole ʻia ke kūlana olakino. Pono ka poʻe huakaʻi e makaʻala i ka maʻi maʻi monkey ma ko lākou mau ʻāina a me nā ʻāpana a pale i ka launa pū me nā rodents a me nā primates.
Inā loaʻa nā ʻano pilikia nui, e nānā pono iā ʻoe iho i kou olakino no 21 mau lā a pale i ka pilina pili me nā poʻe ʻē aʻe. Inā ʻike ʻia nā hōʻailona e like me ka ʻūhū, nā ʻōpala, a i ʻole ke kuni, e ʻimi koke i ka lāʻau lapaʻau a haʻi i ke kauka no nā ʻano kūpono.
Inā ʻike ʻia kekahi lālā o ka ʻohana a i ʻole kekahi hoaaloha i ka maʻi monkeypox, e hana i nā hana pale pilikino, e pale i ka launa pū ʻana me ka mea maʻi, a mai hoʻopā i nā mea a ka mea maʻi i hoʻohana ai, e like me nā lole, nā moena, nā kāwele, a me nā mea pilikino ʻē aʻe. E hōʻalo i ka hoʻokaʻawale ʻana i nā lumi ʻauʻau, a holoi pinepine i nā lima a hoʻokuʻu i nā lumi.
ʻO nā mea hoʻopaneʻe hoʻonaʻauao Monkeypox
ʻO ka Monkeypox diagnostic reagents e kōkua i ka hoʻopaʻa ʻana i ka maʻi ma o ka ʻike ʻana i nā antigens a i ʻole nā antibodies, hiki ke hoʻokaʻawale kūpono a me nā ʻano lapaʻau, a ke pāʻani nei i kahi kuleana nui i ka pale ʻana i nā maʻi maʻi. I kēia manawa, ua hoʻomohala ʻo Anhui DeepBlue Medical Technology Co., Ltd. i nā mea hoʻoponopono diagnostic monkeypox penei:
Monkeypox Antigen Test Kit: Hoʻohana i ke ʻano gula colloidal no ka hōʻiliʻili ʻana i nā mea hoʻohālike e like me ka oropharyngeal swabs, nasopharyngeal swabs, a i ʻole exudates ʻili no ka ʻike. Hōʻoiaʻiʻo ia i ka maʻi ma ka ʻike ʻana i ke alo o nā antigens viral.
Monkeypox Antibody Test Kit: Hoʻohana i ke ʻano gula colloidal, me nā laʻana me ke koko holoʻokoʻa venous, plasma, a i ʻole serum. Hōʻoia ia i ka maʻi ma o ka ʻike ʻana i nā antibodies i hana ʻia e ke kino kanaka a holoholona e kūʻē i ka maʻi monkeypox.
Monkeypox Virus Nucleic Acid Test Kit: Hoʻohana i ke ʻano PCR quantitative fluorescent manawa maoli, me ka laʻana he lesion exudate. Hoʻopaʻa ia i ka maʻi ma o ka ʻike ʻana i ka genome a i ʻole nā ʻāpana gene kikoʻī.
Nā Huahana Hoʻāʻo Monkeypox Testsealabs
Mai ka makahiki 2015, ua hōʻoia ʻia nā reagents diagnostic monkeypox Testsealabs me ka hoʻohana ʻana i nā maʻi virus maoli i nā laboratories haole a ua hōʻoia ʻia ʻo CE ma muli o kā lākou hana paʻa a hilinaʻi. Hoʻopili kēia mau reagents i nā ʻano laʻana like ʻole, e hāʻawi ana i nā ʻano like ʻole a me nā pae kikoʻī, hāʻawi i ke kākoʻo ikaika no ka ʻike ʻana i ka maʻi monkeypox a me ke kōkua maikaʻi ʻana i ka hoʻomalu outbreak. No ka ʻike hou aku e pili ana i kā mākou pahu hōʻike monkeypox, e ʻoluʻolu e nānā: https://www.testsealabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Kaʻina hana hoʻāʻo
E hoʻohana i kahi swab e hōʻiliʻili i ka pus mai ka pustule, e hui pono i loko o ka pustulebuffer, a laila e hoʻopili i kekahi mau kulu i loko o ke kāleka hōʻike. Hiki ke loaʻa ka hopena ma nā ʻanuʻu maʻalahi.
