Gwajin Cutar Cutar Maleriya Ag pf/pv Gwajin Layi Uku

Gwajin Cutar Cutar Maleriya Ag pf/pv Gwajin Layin Tri-Layi Fiyayyen Hoto

Takaitaccen Bayani:

Manufar:
Wannan gwajin yana ba da hanya mai sauri da aminci don gano cutar zazzabin cizon sauro da ta haifarPlasmodium falciparumkumaPlasmodium vivax. Yana gano takamaiman antigens na malaria (kamar HRP-2 don Pf da pLDH don Pv) waɗanda ke cikin jini yayin kamuwa da cuta.

Mabuɗin fasali:

Zane-zanen layi uku:
- Wannan gwajin yana iya gano duka biyunPlasmodium falciparum (Pf)kumaPlasmodium vivax (Pv)cututtuka, tare da layi daban-daban don kowane nau'i da kuma layin sarrafawa don tabbatar da inganci.
Sakamako cikin gaggawa:
- Ana samun sakamako a cikin adalciMinti 15-20, wanda ya sa ya dace da amfani da filin da kuma ganewar asali a yankunan da ke da iyakacin damar yin amfani da kayan aikin dakin gwaje-gwaje.
Babban Hankali da Takamaiman:
- An tsara gwajin don babban hankali da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta na zazzabin cizon sauro, yana ba da ingantaccen sakamako don taimakawa wajen kula da cutar zazzabin cizon sauro.
Sauƙin Amfani:
- Gwajin yana buƙatar ƙaramin horo don yin aiki kuma ya dace don amfani a wurare masu nisa ko ƙayyadaddun saitunan albarkatu.

Aika mana imel Zazzage azaman PDF

Cikakken Bayani

Tags samfurin

Cikakken Bayani:

Nau'in Misali:
- Jini duka (samfurin jinin yatsa ko venipuncture).
Lokacin Ganewa:
- Minti 15-20(ya kamata a fassara sakamakon a cikin mintuna 20; sakamakon bayan wannan lokacin ba shi da inganci).
Hankali da ƙayyadaddun bayanai:
- Hankali:Yawanci> 90% don gano cututtukan Pf da Pv.
- Musamman:Yawanci> 95% don gano Pf da Pv duka.
Yanayin Ajiya:
- Adana tsakanin4°C da 30°C, nesa da hasken rana kai tsaye.
- Kar a daskare.
- Rayuwar rayuwa yawanci tana fitowa dagaWatanni 12 zuwa 24, dangane da umarnin masana'anta.
Fassarar sakamako:
- Sakamako Mai Kyau:
  - Layuka uku na bayyane:
    1. C (Control) layi(yana nuna gwajin inganci).
    2. layin Pf(idan an gano antigens na Plasmodium falciparum).
    3. Layin Pv(idan an gano antigens na Plasmodium vivax).
    - Kasancewar layin Pf da/ko Pv yana nuna kamuwa da cuta tare da nau'ikan zazzabin cizon sauro.

Ka'ida:

Immunochromatographic Assay:
Kaset ɗin gwajin ya ƙunshi marasa motsimonoclonal antibodiesmusamman ga Plasmodium antigens (misali,HRP-2don Pf dapLDHda Pv).

Lokacin da aka shafa jini akan gwajin, idanmaganin zazzabin cizon saurosuna nan, za su ɗaure da ƙwayoyin rigakafi masu haɗin gwal a cikin samfurin, wanda zai motsa tare da membrane na gwaji ta hanyar aikin capillary.
Idan daPlasmodium falciparumAn gano antigen, wani layi mai launi zai samar a cikinlayin Pf.
Idan daPlasmodium vivaxAn gano antigen, wani layi mai launi zai samar a cikinLayin Pv.
TheLayin sarrafawa (C)yana tabbatar da gwajin yana aiki da kyau kuma yana nuna ingancin gwajin.

Abun ciki:

Abun ciki	Adadin	Ƙayyadaddun bayanai
IFU	1	/
Gwada kaset	25	Kowace jakar da aka hatimi mai ɗauke da na'urar gwaji guda ɗaya da na'urar bushewa ɗaya
Diluent na hakar	500μL1 Tube 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Dropper tip	1	/
Swab	/	/

Tsarin Gwaji:


1. Wanke hannu	2. Bincika abubuwan da ke cikin kit kafin gwaji, haɗa da saka fakiti, kaset ɗin gwaji, buffer, swab.	3. Sanya bututun hakar a cikin wurin aiki.	4.peel kashe hatimin tsare-tsare na aluminum daga saman bututun cirewa wanda ke dauke da buffer cirewa.

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5.Ki cire swab a hankali ba tare da taɓa tip ba.Saka gaba ɗaya tip ɗin swab 2 zuwa 3 cm a cikin hancin dama. Lura da tsinkewar hancin hanci. Kuna iya jin haka da yatsun hannu yayin shigar da hancin hanci ko duba. shi a cikin zuciyata. A shafa cikin hancin cikin motsi na madauwari sau 5 na akalla dakika 15,yanzu ki dauko hancin hanci iri daya ki sa a cikin sauran hancin.Swab cikin hancin cikin madauwari sau 5 na akalla dakika 15. Da fatan za a yi gwajin kai tsaye tare da samfurin kuma kada ku yi
barshi tsaye.

6. Sanya swab a cikin bututun hakar. Juya swab na kimanin 10 seconds, Juya swab a kan bututun cirewa, danna kan swab a cikin bututun yayin da yake matse bangarorin bututu don sakin ruwa mai yawa. kamar yadda zai yiwu daga swab.


7. Cire swab daga kunshin ba tare da taɓa padding ba.	8. Mix sosai ta hanyar flicking kasa na bututu. Sanya 3 saukad da samfurin a tsaye a cikin rijiyar samfurin gwajin kaset. Karanta sakamakon bayan minti 15. Lura: Karanta sakamakon a cikin mintuna 20. In ba haka ba, ana ba da shawarar koken gwajin.

Fassarar Sakamako: