Gwajin Cutar Cutar HIV 1/2 Kit ɗin Gwajin Sauri

Gwajin Cutar Gwajin HIV 1/2 Na'urar Gwajin Saurin Fitar Hoton

Takaitaccen Bayani:

Cutar Kariya ta Mutum (HIV)kwayar cuta ce da ke kai hari ga tsarin garkuwar jiki, musamman wanda ke kai hariCD4+ T Kwayoyin(wanda kuma aka sani da ƙwayoyin T-helper), waɗanda ke da mahimmanci don kariyar rigakafi. Idan ba a kula da shi ba, HIV na iya haifar da shiCiwon Immunodeficiency Syndrome (AIDS), yanayin da tsarin garkuwar jiki ya yi rauni sosai kuma ya kasa yaƙar cututtuka da cututtuka.

Ana kamuwa da cutar ta HIV ta hanyar farkojini, maniyyi, ruwan farji, ruwan dubura, kumanono nono. Mafi yawan hanyoyin watsawa sun haɗa da jima'i ba tare da kariya ba, raba gurɓataccen allura, da watsawa uwa zuwa yaro yayin haihuwa ko shayarwa.

Akwai manyan nau'ikan HIV guda biyu:

HIV-1:Mafi na kowa kuma yaduwa nau'in HIV a duniya.
HIV-2:Mafi ƙarancin gama gari, da farko ana samunsa a Yammacin Afirka, kuma yawanci yana da alaƙa da saurin ci gaba zuwa AIDS.

Ganowa da wuri da magani tare damaganin antiretroviral (ART)zai iya taimaka wa masu cutar kanjamau su rayu tsawon rai, lafiyayye kuma rage haɗarin watsawa ga wasu.

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Cikakken Bayani

Tags samfurin

Cikakken Bayani:

Babban Hankali da Takamaiman
An ƙera gwajin don gano daidaitattun ƙwayoyin cuta na HIV-1 da HIV-2, suna ba da ingantaccen sakamako tare da ƙarancin amsawa.
Sakamako cikin gaggawa
Ana samun sakamako a cikin mintuna 15-20, yana ba da damar yanke shawara na asibiti nan da nan da rage lokacin jiran marasa lafiya.
Sauƙin Amfani
Ƙira mai sauƙi da mai amfani, ba buƙatar kayan aiki na musamman ko horo ba. Ya dace don amfani a duka saitunan asibiti da wurare masu nisa.
Nau'o'in Samfuri iri-iri
Gwajin ya dace da cikakken jini, jini, ko plasma, yana ba da sassauci a cikin tarin samfuri da haɓaka kewayon aikace-aikace.
Ƙarfafawa da Aikace-aikacen Filin
Karami mai nauyi da nauyi, sanya kayan gwajin ya dace don saitunan kulawa, dakunan shan magani na wayar hannu, da shirye-shiryen tantance yawan jama'a.

Ka'ida:

Samfurin Tarin
Ana amfani da ƙaramin ƙarar jini, plasma, ko gabaɗayan jini a rijiyar samfurin na'urar, sannan a ƙara ma'aunin buffer don fara aikin gwajin.
Alamar Antigen-Antibody
Gwajin ya ƙunshi recombinant antigens ga duka HIV-1 da HIV-2, waɗanda ba su iya motsi a yankin gwaji na membrane. Idan kwayoyin rigakafi na HIV (IgG, IgM, ko duka biyu) suna cikin samfurin, za su ɗaure ga antigens a kan membrane, samar da wani hadadden antigen-antibody.
Hijira na Chromatographic
Ƙungiyar antigen-antibody tana motsawa tare da membrane ta hanyar aikin capillary. Idan kwayoyin rigakafi na HIV suna nan, hadaddun za su ɗaure da layin gwaji (T line), yana samar da layin launi mai gani. Sauran reagents sunyi ƙaura zuwa layin sarrafawa (layin C) don tabbatar da ingancin gwajin.
Tafsirin sakamako
- Layi biyu (Layin T + C):Kyakkyawan sakamako, yana nuna kasancewar ƙwayoyin rigakafin HIV-1 da/ko HIV-2.
- Layi ɗaya (Layin C kawai):Sakamako mara kyau, yana nuna ba a iya gano ƙwayoyin rigakafin HIV.
- Babu layi ko layin T kawai:Sakamakon mara inganci, yana buƙatar maimaita gwaji.

Abun ciki:

Abun ciki	Adadin	Ƙayyadaddun bayanai
IFU	1	/
Gwada kaset	1	Kowace jakar da aka hatimi mai ɗauke da na'urar gwaji guda ɗaya da na'urar bushewa ɗaya
Diluent na hakar	500μL1 Tube 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Dropper tip	1	/
Swab	1	/

Tsarin Gwaji:


1. Wanke hannu	2. Bincika abubuwan da ke cikin kit kafin gwaji, haɗa da saka fakiti, kaset ɗin gwaji, buffer, swab.	3. Sanya bututun hakar a cikin wurin aiki.	4.peel kashe hatimin tsare-tsare na aluminum daga saman bututun cirewa wanda ke dauke da buffer cirewa.

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5.Ki cire swab a hankali ba tare da taɓa tip ba.Saka gaba ɗaya tip ɗin swab 2 zuwa 3 cm a cikin hancin dama. Lura da tsinkewar hancin hanci. Kuna iya jin haka da yatsun hannu yayin shigar da hancin hanci ko duba. shi a cikin zuciyata. A shafa cikin hancin cikin motsi na madauwari sau 5 na akalla dakika 15,yanzu ki dauko hancin hanci iri daya ki sa a cikin sauran hancin.Swab cikin hancin cikin madauwari sau 5 na akalla dakika 15. Da fatan za a yi gwajin kai tsaye tare da samfurin kuma kada ku yi
barshi tsaye.

6. Sanya swab a cikin bututun hakar. Juya swab na kimanin 10 seconds, Juya swab a kan bututun cirewa, danna kan swab a cikin bututun yayin da yake matse bangarorin bututu don sakin ruwa mai yawa. kamar yadda zai yiwu daga swab.


7. Cire swab daga kunshin ba tare da taɓa padding ba.	8. Mix sosai ta hanyar flicking kasa na bututu. Sanya 3 saukad da samfurin a tsaye a cikin rijiyar samfurin gwajin kaset. Karanta sakamakon bayan minti 15. Lura: Karanta sakamakon a cikin mintuna 20. In ba haka ba, ana ba da shawarar koken gwajin.

Fassarar Sakamako: