SARS-CoV-2 Neutralizing antibody Test Cassette

Don ƙididdige ƙimar cutar Coronavirus 2019 (2019-nCOV ko COVID-19) neutralizing antibody a cikin jini/plasma/jinin ɗan adam.

Don ƙwararre In Vitro Diagnostic Amfani Kawai

【YADDA AKE NUFI】

Cassette na gwaji na SARS-CoV-2 Neutralizing antibody shine saurin chromatographic

Immunoassay don gano ƙimar ƙarancin rigakafin cutar Coronavirus 2019 a cikin jinin ɗan adam, jini, ko plasma azaman taimako a cikin matakan kimantawa na ɗan adam anti-novel coronavirus neutralizing antibody titer.

Dabbobi masu shayarwa. Halin γ galibi yana haifar da cututtukan tsuntsaye. CoV galibi ana ɗaukarsa ta hanyar hulɗa kai tsaye tare da ɓoye ko ta iska da ɗigon ruwa. Akwai kuma shaidar cewa ana iya yada ta ta hanyar fecal-baki.

Mummunan ciwo mai tsanani na numfashi na coronavirus 2 (SARS-CoV-2, ko 2019-nCoV) kwayar cutar RNA ce mai lullube wacce ba ta da ma'ana. Ita ce sanadin cutar coronavirus 2019 (COVID-19), wacce ke yaduwa a cikin mutane.

SARS-CoV-2 yana da sunadaran tsari da yawa waɗanda suka haɗa da karu (S), ambulaf (E), membrane (M) da nucleocapsid (N). Sunadaran karu (S) ya ƙunshi yanki mai ɗaure mai karɓa (RBD), wanda ke da alhakin gane mai karɓar saman tantanin halitta, angiotensin yana canza enzyme-2 (ACE2). An gano cewa RBD na furotin na SARS-CoV-2 S yana yin hulɗa da ƙarfi tare da mai karɓar ACE2 na ɗan adam wanda ke haifar da endocytosis a cikin ƙwayoyin runduna na huhu mai zurfi da kwafi.

Kamuwa da SARS-CoV-2 yana fara amsawar rigakafi, wanda ya haɗa da samar da ƙwayoyin rigakafi a cikin jini. Magungunan da aka ɓoye suna ba da kariya daga kamuwa da cuta a nan gaba daga ƙwayoyin cuta, saboda suna kasancewa a cikin tsarin jini na tsawon watanni zuwa shekaru bayan kamuwa da cuta kuma za su ɗaure da sauri da ƙarfi ga ƙwayoyin cuta don toshe kutsewar salula da maimaitawa. Waɗannan ƙwayoyin rigakafi ana kiransu antibodies neutralizing.

【 TATTAUNAWA DA SHIRI】

1.The SARS-CoV-2 Neutralizing antibody Test Cassette an yi niyya ne don amfani da cikakken jinin ɗan adam, serum ko samfuran plasma kawai.

2.Kawai bayyanannu, samfurori marasa hemolyzed ana ba da shawarar don amfani da wannan gwajin. Ya kamata a raba maganin jini ko plasma da wuri-wuri don guje wa hemolysis.

3.Yi gwaji nan da nan bayan tattara samfurin. Kada a bar samfurori a zafin jiki na tsawon lokaci. Ana iya adana samfuran jini da jini a 2-8 ° C har zuwa kwanaki 3. Don adanawa na dogon lokaci, samfuran jini ko plasma yakamata a kiyaye su ƙasa da 20 ° C. Duk jinin da aka tattara ta hanyar venipuncture yakamata a adana shi a 2-8 ° C idan za a gudanar da gwajin cikin kwanaki 2 bayan an tattara. samfurori. Dukkan jinin da aka tattara ta hannun yatsa yakamata a gwada shi nan da nan.

4.Ya kamata a yi amfani da kwantena da ke ɗauke da magungunan kashe jini kamar EDTA,citrate, ko heparin don ajiyar jini gaba ɗaya.Kawo samfurori zuwa zafin jiki kafin gwaji.

5. Dole ne a narke gaba ɗaya samfuran daskararre kuma a gauraye su da kyau kafin a gwada su.

da narke samfurori.

6. Idan za a aika samfurori, shirya su bisa ga duk ka'idojin sufuri.

na etiological agents.

7.Icteric, lipemic, hemolyzed, zafi bi da kuma gurbata sera iya haifar da kuskure sakamakon.

8.Lokacin da ake tattara jinin ɗan yatsa tare da lancet da pad na barasa, da fatan za a jefar da digon farko na

duka jini.

1. Kawo jakar zuwa zafin jiki kafin buɗewa. Cire na'urar gwaji daga jakar da aka rufe kuma amfani da shi da wuri-wuri.

2. Sanya na'urar gwajin akan wuri mai tsabta da matakin.

Don samfuran jini ko Plasma: Amfani da Micropipette, da kuma canja wurin 5ul serum/plasma zuwa samfurin rijiyar na'urar gwaji, sa'an nan kuma ƙara 2 digo na buffer, da kuma fara mai lokaci.

Ga Cikakken Jini (Venipuncture/Fingerstick) Samfura: Dauki yatsa sannan ka matse yatsa a hankali, yi amfani da pipette filastik ɗin da aka tanadar don tsotse 10ul na jini gaba ɗaya zuwa layin 10ul na pipette filastik, sannan a tura shi zuwa rami samfurin na'urar gwajin (idan girman jinin duka ya wuce alamar, Da fatan za a saki jinin da ya wuce kima a cikin pipette), sannan ƙara digo 2 na buffer, sannan fara mai ƙidayar lokaci. Lura: Hakanan ana iya amfani da samfurori ta amfani da micropipette.

3. Jira layin (s) masu launi ya bayyana. Karanta sakamakon a minti 15. Kada ka fassara sakamakon bayan minti 20.