SARS-COV-2 CIGABA DA ITA
Na ubuƘa'ida】
The SARS-Cov-2 neutrarancin Kit ɗin Anibody Gano yana dogara ne akan Tallafin Eli Mehiya.
Ta amfani da tsarkakakken yanki mai ƙarfi (rBD), furotin daga furotin karara na hoto (s) da kuma sel mai amfani
Recepor Ace2, an tsara wannan gwajin don kwaikwayon kwayar cutar ta kwaikwayi hulɗa.
Caliban da ke ingancin, ingancin iko, da magani ko samfuran plasma ana haɗuwa da kyau a dilowe
Buffer dauke da hace-hrp conjugate da yawan lokuta a cikin kananan shambura. Sannan ana tura su gauraya a ciki
Riplate riplate dauke da rashin tsari yana haifar da wani yanki mai-cov-2 rBD (RBD) don
shiryawa. A yayin shiryawa na minti 30, RBD takamaiman antibody a cikin calibrassors, QC da
Samfurori za su yi gasa tare da hace-hrp na takamaiman da ke ɗaure RBD wanda aka ɓata RBD a cikin rijiyoyin. Bayan
A shiryawa, rijiyoyin suna wanke sau 4 don cire Unbound Hace2-HRP Conjugate. Bayani na
An kara TMB kuma an kara shi na minti 20 a zazzabi a daki, wanda ya haifar da ci gaban a
launin shuɗi. An dakatar da ci gaban launi tare da Bugu da kari na 1n hcl, da kuma tunawa shine
auna abin da ake ciki a 450 nm. Tsanani na launi kafa daidai ne ga
Adadin Enzyme Ba tare da izini ba, kuma yana da alaƙa da adadin ƙa'idodi masu mahimmanci kamar yadda.
Ta hanyar kwatanta da calibrators clebritors da aka bayar da calibsed, da maida hankali
Neutralizing abubuwan rigakafi a cikin samfurin da ba a sani ba an lasafta shi.
Na ubuAbubuwan da ake buƙata amma ba a bayar ba】
1. Distilled ko ruwa mai narkewa
2
3. Shawara bututun bututun
4. Mai karatu mai amfani wanda ya iya karanta tunawa a 450nm.
5. Takaddar takarda
6. Takarda Takarda
7. Vortex Murris ko daidai
Na ubuSamfuran samfuran da ajiya】
1. Serum da samfurori plasma da aka tattara a cikin bututu wanda ke dauke da K2-Edta za a iya amfani da wannan kit.
2. Dole ne a adana samfuran samfurori kuma ana iya adana shi har zuwa awanni 48 a 2 ° C - 8 ° C - 8 ° C - 8 ° C kafin ya tabbatar.
Mane samfurori da aka ɗauka na tsawon lokaci (har zuwa watanni 6) ya kamata ya daskarewa sau ɗaya kawai a -20 ° C kafin assday.
Guji maimaita maimaita-narkewa.
Kasawa
Na ubuYaduwar Reagent】
1. Duk reagents dole ne a cire daga sanyali kuma a yarda su koma zazzabi a daki kafin amfani
(20 ° zuwa 25 ° C). Ajiye duk reagents a cikin firiji da sauri bayan amfani.
2. Duk samfurori da sarrafawa ya kamata a faɗaɗa kafin amfani.
3
Buffer. Misali, tsarma 100 μL na Hace2-hRP mai da hankali tare da 5.0mL na HRP Diluter to
Yi maganin hrace-hrp.
4. 1 × Wanke Magani na Wanke: Tsara 20 × 20 wanke tare da Dama ko Distilled ruwa tare da
rabo na 1:19. Misali, tsarma 20 ml na 20 × na wash na 20 tare da 380 ml na deonized ko
Distilled ruwa don yin 400 ml na shash 1 × na wanka.
Na ubuHanya gwaji】
1. A cikin bututun daban, oquot 120μl na maganin tattalin hanci-hrp.
2. Addara 6 μL na Calibrassors, Samfuran da ba a sansu ba, iko mai inganci a cikin kowane bututu kuma Mix da kyau.
3
a tsarin gwajin farawa.
3. Rufe farantin tare da farantin farantin da kuma incubate da 37 ° C na mintina 30.
4. Cire mai siyar da farantin kuma wanke farantin tare da kusan 300 μL na 1 μl na Wash bayani kowace kyau sau hudu.
5. Matsa farantin a tawul takarda don cire ruwa mai saura a cikin rijiyoyin bayan wanke wanke wanke.
6. Addara 100 μL na TMB bayani ga kowane rijiya kuma yana sanya farantin a cikin duhu a 20 - 25 ° C na mintina 20.
7. Addara 50 μL na dakatar da bayani ga kowane rijiya don dakatar da.
8. Karanta tunawa da abin sha a cikin mai karatu na microplate a 450 NM a cikin minti 10 (630nm kamar kayan aiki ne
shawarar da aka ba da shawarar don mafi girma aikin).