Sars-CoV-2 Neutralizing Antibody Detection Kit (ELISA)
【AMFANI DA NUFIN】
SARS-CoV-2 Neutralizing Antibody Gano Kit is a Competitive Enzyme-Linked Immunosorbent Assay (ELISA) wanda aka yi niyya don gano ƙimar ƙima da ƙima na jimlar kawar da ƙwayoyin rigakafi zuwa SARS-CoV-2 a cikin jini da jini. Za a iya amfani da Kit ɗin Neutralizing Antibody Detection Kit na SARS-CoV-2 azaman taimako don gano mutanen da ke da amsawar rigakafi ga SARS-CoV-2, yana nuna kamuwa da cuta kwanan nan ko kafin. Bai kamata a yi amfani da Kit ɗin Gano Maganin Neutralizing Antibody na SARS-CoV-2 don tantance kamuwa da cutar SARS-CoV-2 mai tsanani ba.
【GABATARWA】
Kwayoyin cutar Coronavirus yawanci suna haifar da martanin antibody. Adadin juyawa a cikin marasa lafiya na COVID-19 shine 50% da 100% a rana ta 7 da 14 bayan bayyanar alamun, bi da bi. Don gabatar da ilimi, daidaitaccen ƙwayar cuta mai hana ƙwayoyin cuta a cikin jini an gane shi azaman manufa don tantance ingancin antibody da babban taro na antibody neutralizing yana nuna ingantaccen ingantaccen kariya. An gane Gwajin Rage Tsabtace Plaque (PRNT) azaman ma'aunin zinare don gano maganin kashe ƙwayoyin cuta. Koyaya, saboda ƙarancin kayan aikin sa da mafi girman buƙatu don aiki, PRNT ba ta da amfani don babban sikelin serodiagnosis da kimantawar rigakafin. The SARS-CoV-2 Neutralizing Antibody Gane Kit dogara ne a kan Gasar Enzyme-Linked Immunosorbent Assay (ELISA), wanda zai iya gano antibody neutralizing a cikin samfurin jini da kuma samun damar musamman matakan maida hankali na irin wannan antibody.
【Tsarin Gwaji】
1.A cikin tubes daban-daban, aliquot 120μL na maganin hACE2-HRP da aka shirya.
2.Add 6 μL na calibrators, samfurori da ba a sani ba, masu sarrafawa masu inganci a cikin kowane bututu da haɗuwa da kyau.
3.Transfer 100μL na kowane cakuda da aka shirya a cikin mataki na 2 a cikin rijiyoyin microplate daidai bisa ga tsarin gwajin da aka riga aka tsara.
3.Rufe farantin tare da Plate Sealer kuma a sanya shi a 37 ° C na minti 60.
4.Cire Plate Seler kuma a wanke farantin tare da kimanin 300 μL na 1 × Wash Solution da rijiyar har sau hudu.
5.Taɓa farantin a kan tawul ɗin takarda don cire ragowar ruwa a cikin rijiyoyin bayan matakan wankewa.
6.Ƙara 100 μL na Maganin TMB zuwa kowace rijiya da kuma sanya farantin cikin duhu a 20 - 25 ° C na minti 20.
7.Ƙara 50 μL na Tsaida Magani zuwa kowace rijiya don dakatar da amsawa.
8. Karanta abin sha a cikin mai karanta microplate a 450 nm a cikin mintuna 10 (630nm kamar yadda aka ba da shawarar kayan haɗi don mafi girman aiki daidai.
