Mataki Daya SARS-CoV2(COVID-19) Gwajin IgG/IgM
Amfani da Niyya
Mataki na daya na SARS-CoV2 (COVID-19) Gwajin IgG/IgM shine saurin chromatographic immunoassay don gano ingantattun ƙwayoyin rigakafi (IgG da IgM) zuwa ƙwayar cuta ta COVID-19 a cikin Cikakken Jini / Serum / Plasma don taimakawa wajen gano cutar COVID -19 kamuwa da cuta.
Takaitawa
Kwayoyin cutar Corona ƙwayoyin cuta ne na RNA lulluɓe waɗanda ke yaɗuwa a tsakanin mutane, sauran dabbobi masu shayarwa, da tsuntsaye kuma suna haifar da cututtukan numfashi, ciki, hanta da cututtukan neurologic. An san nau'in kwayar cutar corona guda bakwai suna haifar da cutar ɗan adam. Kwayoyin cuta guda hudu-229E. OC43. NL63 da HKu1- suna da yawa kuma yawanci suna haifar da alamun mura na gama gari a cikin mutane masu ƙarfin rigakafi. 19)- asalinsu zoonotic ne kuma ana danganta su da rashin lafiya wani lokaci. IgG da lgM rigakafi zuwa 2019 Novel Coronavirus za a iya gano su tare da makonni 2-3 bayan fallasa. lgG ya kasance tabbatacce, amma matakin antibody yana raguwa akan kari.
Ka'ida
Mataki na daya SARS-CoV2 (COVID-19) IgG/IgM (Dukkan Jini/Serum/Plasma) gwaji ne na immunochromatographic na gefe. Gwajin yana amfani da anti-human lgM antibody (layin gwajin IgM), anti-human lgG(layin gwaji lgG da goat anti-zomo igG (layin sarrafawa C) wanda ba a iya motsi a kan tsiri na nitrocellulose. Kushin haɗin gwal mai launin burgundy ya ƙunshi zinare colloidal wanda aka haɗa don sake haɗuwa. COVID-19 antigens conjugated da colloid zinariya (COVID-19 conjugates da zomo lgG-zinariya conjugates. Lokacin da wani samfurin bi da assay buffer aka kara da samfurin da kyau, IgM &/ko lgG antibodies idan akwai, za su ɗaure zuwa COVID-19 conjugates yin. Wannan hadaddun yana yin ƙaura ta hanyar nitrocellulose membrane ta hanyar aikin capillary Lokacin da hadaddun ya hadu da layin rigakafi mai dacewa (anti-human IgM &/ko anit-human lgG) hadaddun yana kama da bandeji mai launin burgundy. Sakamakon gwajin amsawa.
Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna ƙungiyar burgundy mai launi na immunocomplex goat anti rabbit IgG/ rabbit lgG-gold conjugate ba tare da la'akari da ci gaban launi akan kowane rukunin gwajin ba. In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
Adana da Kwanciyar hankali
- Ajiye kamar yadda aka kunshe a cikin jakar da aka rufe a zafin daki ko firiji (4-30 ℃ ko 40-86 ℉). Na'urar gwajin ta tsaya tsayin daka ta ranar ƙarewar da aka buga akan jakar da aka hatimi.
- Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.
Ƙarin Kayan Aiki na Musamman
Kayayyakin da Aka Samar:
| .Na'urorin gwaji | . Zazzage samfurin da za a iya zubarwa |
| . Buffer | . Saka kunshin |
Kayayyakin da ake buƙata Amma Ba a Samar da su ba:
| . Centrifuge | . Mai ƙidayar lokaci |
| . Alcohol Pad | . Akwatunan tarin samfurori |
Matakan kariya
☆ Don ƙwararrun in vitro diagnostic amfani kawai. Kada ku yi amfani bayan ranar karewa.
☆ Kada ku ci, ku sha ko shan taba a wurin da ake sarrafa samfurori da kayan aiki.
☆ Yi amfani da duk samfuran kamar suna ɗauke da ƙwayoyin cuta.
☆ Kula da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta a cikin duk hanyoyin kuma bi ƙa'idodin ƙa'idodi don zubar da samfuran daidai.
☆ Sanya tufafin kariya kamar su rigar dakin gwaje-gwaje, safar hannu da za a iya zubar da su da kariya ta ido lokacin da aka tantance samfurori.
☆ Bi daidaitattun ƙa'idodin amincin halittu don sarrafawa da zubar da yuwuwar abubuwan da ba su da lahani.
☆ Danshi da zafin jiki na iya yin illa ga sakamako.
Tarin Samfura da Shirye
1. Ana iya yin gwajin SARS-CoV2 (COVID-19)IgG/IgM akan Jini duka /Magunguna / Plasma.
2. Don tattara duka jini, jini ko samfuran plasma bin hanyoyin gwajin asibiti na yau da kullun.
3. Ya kamata a yi gwaji nan da nan bayan an tattara samfurin. Kar a bar samfuran a zafin jiki na tsawon lokaci. Don ajiya na dogon lokaci, samfurori ya kamata a ajiye su a ƙasa -20 ℃. Dole ne a adana cikakken jini a 2-8 ℃ idan za a gudanar da gwajin a cikin kwanaki 2 na tarin. Kada a daskare duka samfuran jini.
4. Kawo samfurori zuwa zafin jiki kafin gwaji. Daskararrun samfuran dole ne a narke gaba ɗaya kuma a gauraye su da kyau kafin gwaji. Kada a daskare samfuran kuma a narke akai-akai.
Tsarin Gwaji
1. Bada damar gwajin, samfuri, buffer da/ko sarrafawa don isa dakin zafin jiki 15-30℃ (59-86℉) kafin gwaji.
2. Kawo jakar zuwa zafin jiki kafin buɗe shi. Cire na'urar gwajin daga jakar da aka rufe kuma amfani da shi da wuri-wuri.
3. Sanya na'urar gwajin a kan tsaftataccen wuri mai ma'ana.
4. Rike digo a tsaye kuma canja wurin digo 1 na samfurin (kimanin 10μl) zuwa samfurin da kyau (S) na na'urar gwaji, sannan ƙara digo 2 na buffer (kimanin 70μl) kuma fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.
5. Jira layin (s) masu launi ya bayyana. Karanta sakamako a minti 15. Kar a fassara sakamakon bayan mintuna 20.
Bayanan kula:
Aiwatar da isasshen adadin samfur yana da mahimmanci don ingantaccen sakamakon gwaji. Idan ba a lura da ƙaura (jikawar membrane) a cikin taga gwajin bayan minti ɗaya, ƙara ƙarin digo na buffer guda ɗaya a cikin samfurin da kyau.
Tafsirin Sakamako
Mai kyau:Layin sarrafawa da aƙalla layin gwaji ɗaya ya bayyana akan membrane. Bayyanar layin gwajin T2 yana nuna kasancewar COVID-19 takamaiman ƙwayoyin rigakafin IgG. Bayyanar layin gwajin T1 yana nuna kasancewar COVID-19 takamaiman ƙwayoyin rigakafin IgM. Kuma idan duka layin T1 da T2 sun bayyana, yana nuna cewa kasancewar duka biyun COVID-19 takamaiman ƙwayoyin rigakafin IgG da IgM. Ƙananan ƙaddamarwar antibody shine, mafi raunin layin sakamakon shine.
Mara kyau:Layi mai launi ɗaya yana bayyana a cikin yankin sarrafawa(C) .Babu wani layi mai launi da ya bayyana a yankin layin gwaji.
Ba daidai ba:Layin sarrafawa ya kasa bayyana. Rashin isassun samfurin ƙira ko dabarun ƙa'ida ba daidai ba shine mafi kusantar dalilai na gazawar layin sarrafawa. Bita tsarin kuma maimaita gwajin tare da sabuwar na'urar gwaji. Idan matsalar ta ci gaba, daina amfani da kayan gwajin nan da nan kuma tuntuɓi mai rarrabawa na gida.
Iyakance
1.Gwajin SARS-CoV2 (COVID-19) IgG/IgM don amfani ne kawai don gano cutar ta in vitro. Ya kamata a yi amfani da gwajin don gano ƙwayoyin rigakafin COVID-19 a cikin Jini duka / Serum / Plasma kawai. Babu ƙimar ƙima ko ƙimar karuwa a cikin 2. Kwayoyin rigakafin COVID-19 da za a iya tantance su ta wannan gwajin ingancin.
3. Kamar yadda yake tare da duk gwaje-gwajen bincike, duk sakamakon dole ne a fassara shi tare da wasu bayanan asibiti da ke samuwa ga likita.
4. Idan sakamakon gwajin ya kasance mummunan kuma bayyanar cututtuka na asibiti ya ci gaba, ana bada shawarar ƙarin gwaji ta amfani da wasu hanyoyin asibiti. Wani mummunan sakamako ba ya hana kowane lokaci yiwuwar kamuwa da cutar ta COVID-19.
Bayanin Nunin
Takaddun girmamawa
Bayanin Kamfanin
Mu, Hangzhou Testsea Biotechnology Co., Ltd ƙwararren ƙwararren ƙwararren masanin ilimin halittu ne mai saurin girma wanda ya ƙware a cikin bincike, haɓakawa, masana'antu da rarraba kayan gwajin in-vitro diagnostic (IVD) da kayan aikin likita.
Kayan aikin mu shine GMP, ISO9001, da ISO13458 bokan kuma muna da amincewar CE FDA. Yanzu muna sa ran yin haɗin gwiwa tare da ƙarin kamfanoni na ketare don ci gaban juna.
Muna samar da gwajin haihuwa, gwaje-gwajen cututtuka, gwaje-gwajen cin zarafin kwayoyi, gwaje-gwajen alamun zuciya, gwajin alamar ƙari, gwajin abinci da aminci da gwajin cututtukan dabbobi, bugu da ƙari, alamar mu ta TESTSEALABS ta shahara a kasuwannin gida da na ketare. Mafi kyawun inganci da farashi mai kyau yana ba mu damar ɗaukar sama da kashi 50% na hannun jarin cikin gida.
Tsarin Samfur
1.Shirya
2. Rufe
3.Cross membrane
4.Yanke tsiri
5.Majalisi
6.Kira jaka
7. Rufe jakunkuna
8.Buɗe akwatin
9. Kunshi
