Kwayar cutar Monkeypox (MPV) Kayan Gane Acid Nucleic
GABATARWA
Ana amfani da kit ɗin don gano ingancin ingancin in vitro na cututtukan da ake zargin sun kamu da cutar ta Monkeypox (MPV), ƙwayoyin cuta masu tari da sauran lokuta waɗanda ke buƙatar gano cutar kamuwa da cutar ta Monkeypox.
Ana amfani da kit ɗin don gano ƙwayar f3L na MPV a cikin swabs na makogwaro da samfuran swab na hanci.
Sakamakon gwajin wannan kit ɗin don tunani ne na asibiti kawai kuma bai kamata a yi amfani da shi azaman ma'auni kaɗai don ganewar asibiti ba. Ana ba da shawarar yin nazari mai zurfi game da yanayin da ya danganci asibiti na mai haƙuri
bayyanar da sauran gwaje-gwajen dakin gwaje-gwaje.
Amfani da Niyya
Nau'in Assay | swabs na makogwaro da hanci |
Nau'in gwaji | Mai inganci |
Gwaji kayan | PCR |
Girman fakitin | 48 gwaje-gwaje / 1 akwatin |
Yanayin ajiya | 2-30 ℃ |
Rayuwar rayuwa | watanni 10 |
FALALAR KIRKI
Ka'ida
Wannan kit ɗin yana ɗaukar takamaiman jerin abubuwan da aka kiyaye na MPV f3L gene a matsayin yankin da aka yi niyya. Ana amfani da fasaha na PCR mai saurin haske na ainihin lokaci da fasahar sakin nucleic acid don saka idanu akan kwayar nucleic acid ta hanyar canjin siginar kyalli na samfuran haɓakawa. Tsarin ganowa ya haɗa da kulawar inganci na ciki, wanda ake amfani da shi don saka idanu ko akwai masu hana PCR a cikin samfurori ko kuma an ɗauki sel a cikin samfurori, wanda zai iya hana mummunan halin da ake ciki.
BABBAN ABUBUWA
Kit ɗin ya ƙunshi reagents don sarrafa gwaje-gwaje 48 ko sarrafa inganci, gami da abubuwan da ke biyowa:
Reagen A
Suna | Manyan abubuwan da aka gyara | Yawan |
Gano MPV reagent | Tushen dauki ya ƙunshi Mg2+, f3L gene / Rnase P na farko bincike, amsa buffer, Taq DNA enzyme. | 48 gwaje-gwaje |
ReagentB
Suna | Manyan abubuwan da aka gyara | Yawan |
MPV Kyakkyawan Sarrafa | Ya ƙunshi guntun manufa na MPV | 1 tube |
MPV Sarrafa mara kyau | Ba tare da guntun manufa na MPV ba | 1 tube |
DNA saki reagent | Reagent ya ƙunshi Tris, EDTA da Triton. | 48pcs |
Maimaita reagent | DEPC ruwan magani | 5ML |
Lura: Abubuwan da aka haɗa na lambobi daban-daban ba za a iya amfani da su ba
【Yanayin Ajiya Da Rayuwar Shelf】
1.Reagent A / B za a iya adana a 2-30 ° C, da kuma shiryayye rayuwa ne 10 watanni.
2.Don Allah a buɗe murfin bututun gwajin kawai lokacin da kuka shirya don gwajin.
3.Kada ku yi amfani da bututun gwaji fiye da ranar karewa.
4.Kada ku yi amfani da bututun ganowa.
【Abubuwan da ake Aiwatar da su】
Dace da dacewa da tsarin bincike na LC480 PCR, Gentier 48E Atomatik PCR tsarin bincike, ABI7500 PCR tsarin bincike.
【Samfuran Bukatun】
1.Amfani nau'in samfurin: samfurin swabs makogwaro.
2.Sampling Magani:Bayan tabbatarwa, ana ba da shawarar yin amfani da saline na yau da kullun ko bututun adana ƙwayar cuta wanda Hangzhou Testsea ilmin halitta ya samar don tarin samfura.
makogwaro:shafa tonsils na pharyngeal biyu da bangon pharyngeal na baya tare da swab samfurin bakararre mai yuwuwa, nutsar da swab cikin bututu mai ɗauke da maganin samfurin 3mL, jefar da wutsiya, sannan ƙara murfin bututu.
3.Sample ajiya da bayarwa:Ya kamata a gwada samfuran da za a gwada da wuri-wuri. The sufuri zafin jiki ya kamata a kiyaye a 2 ~ 8 ℃.The samfurori da za a iya gwada a cikin 24 hours za a iya adana a 2 ℃ ~ 8 ℃ da kuma idan samfurori ba za a iya gwada a cikin 24 hours, shi ya kamata a adana a kasa da ko daidai. zuwa -70 ℃ (idan babu yanayin ajiya na -70 ℃, ana iya adana shi a -20 ℃ na ɗan lokaci), guje wa maimaitawa.
daskarewa da narkewa.
4.Proper samfurin tarin, ajiya, da sufuri suna da mahimmanci ga aikin wannan samfurin.
【Hanyar Gwaji】
1.Sample aiki da samfurin ƙari
1.1 Samfurin sarrafawa
Bayan haɗa maganin samfurin sama tare da samfurori, ɗauki 30μL na samfurin a cikin bututun sakewa na DNA kuma a haɗa shi daidai.
1.2 Loading
Dauki 20μL na reconstitution reagent kuma ƙara shi zuwa MPV gano reagent, ƙara 5μL na sama sarrafa samfurin (The tabbatacce iko da korau iko za a sarrafa a layi daya tare da samfurori), rufe tube hula, centrifuge shi a 2000rpm for 10 seconds.
2. PCR haɓakawa
2.1 Load da farantin PCR da aka shirya / bututu zuwa kayan aikin PCR mai haske, iko mara kyau da ingantaccen iko za a saita don kowane gwaji.
2.2 Saitin tashar Fluorescent:
1) Zaɓi tashar FAM don gano MPV;
2) Zaɓi tashar HEX / VIC don gano abubuwan sarrafawa na ciki;
3.Nazarin sakamako
Saita layin tushe sama da mafi girman madaidaicin madaidaicin lanƙwasa mai kyalli.
4.Tsarin sarrafawa
4.1 Korau iko: Babu ƙimar Ct da aka gano a cikin FAM, tashar HEX / VIC, ko Ct :40;
4.2 Kyakkyawan sarrafawa: A cikin FAM, tashar HEX / VIC, Ct≤40;
4.3 Abubuwan da ke sama yakamata a cika su a cikin gwaji iri ɗaya, in ba haka ba sakamakon gwajin ba shi da inganci kuma gwajin yana buƙatar maimaitawa.
【Yanke darajar】
Ana ɗaukar samfurin a matsayin tabbatacce lokacin: Jerin Target Ct≤40, Tsarin sarrafa ciki Ct≤40.
【Tafsirin sakamako】
Da zarar an wuce ingancin kulawa, masu amfani yakamata su bincika idan akwai madaidaicin haɓakawa ga kowane samfuri a cikin tashar HEX / VIC, idan akwai kuma tare da Ct≤40, yana nuna nasarar haɓakar ƙwayar sarrafawa ta ciki kuma wannan takamaiman gwajin yana da inganci. Masu amfani za su iya ci gaba zuwa bincike mai zuwa:
3.Don samfurori tare da haɓakawa na tsarin kulawa na ciki ya kasa (HEX / VIC
tashar, Ct>40, ko babu ƙararrawa kwana), ƙananan nauyin ƙwayar cuta ko kasancewar mai hana PCR zai iya zama dalilin rashin nasara, jarrabawar ya kamata a maimaita daga tarin samfurin;
4.Don samfurori masu kyau da ƙwayoyin cuta na al'ada, sakamakon kula da ciki ba ya tasiri;
Don samfuran da aka gwada ba su da kyau, ana buƙatar sarrafa iko na ciki don gwada inganci in ba haka ba sakamakon gabaɗayan ba daidai ba ne kuma ana buƙatar maimaita jarrabawa, farawa daga matakin tarin samfurin.
Bayanin Nunin
Bayanin Kamfanin
Mu, Hangzhou Testsea Biotechnology Co., Ltd ƙwararren ƙwararren ƙwararren masanin ilimin halittu ne mai saurin girma wanda ya ƙware a cikin bincike, haɓakawa, masana'antu da rarraba kayan gwajin in-vitro diagnostic (IVD) da kayan aikin likita.
Kayan aikin mu shine GMP, ISO9001, da ISO13458 bokan kuma muna da amincewar CE FDA. Yanzu muna sa ran yin haɗin gwiwa tare da ƙarin kamfanoni na ketare don ci gaban juna.
Muna samar da gwajin haihuwa, gwaje-gwajen cututtuka, gwaje-gwajen cin zarafin kwayoyi, gwaje-gwajen alamun zuciya, gwajin alamar ƙari, gwajin abinci da aminci da gwajin cututtukan dabbobi, bugu da ƙari, alamar mu ta TESTSEALABS ta shahara a kasuwannin gida da na ketare. Mafi kyawun inganci da farashi mai kyau yana ba mu damar ɗaukar sama da kashi 50% na hannun jarin cikin gida.
Tsarin Samfur
1.Shirya
2. Rufe
3.Cross membrane
4.Yanke tsiri
5.Majalisi
6.Kira jaka
7. Rufe jakunkuna
8.Buɗe akwatin
9. Kunshi
Hana Wani Sabon Bala'i: Shirya Yanzu Kamar Yadda Cutar Cutar Biri Ke Yaɗuwa
A ranar 14 ga Agusta, Hukumar Lafiya ta Duniya (WHO) ta ba da sanarwar cewa barkewar cutar sankara ta biri ta zama "Gaggawar Lafiyar Jama'a ta Damuwa ta Duniya." Wannan shi ne karo na biyu da hukumar ta WHO ke fitar da mafi girman matakin fadakarwa game da barkewar cutar sankarau tun daga watan Yulin 2022.
A halin yanzu, bullar cutar kyandar biri ta bazu daga Afirka zuwa Turai da Asiya, inda aka tabbatar da bullar cutar a kasashen Sweden da Pakistan.
Bisa kididdigar da hukumar kula da lafiya ta Afirka CDC ta fitar, a bana, kasashe 12 na kungiyar Tarayyar Afirka sun ba da rahoton bullar cutar kyandar biri guda 18,737, ciki har da mutane 3,101 da aka tabbatar sun kamu da cutar, da 15,636 da ake zargi da kamuwa da cutar, da kuma mutuwar mutane 541, tare da asarar rayuka 2.89%.
01 Menene cutar sankarau?
Monkeypox (MPX) cuta ce ta zoonotic ta kwayar cutar da kwayar cutar kyandar biri ke haifarwa. Ana iya yada shi daga dabbobi zuwa mutane, da kuma tsakanin mutane. Alamun alamun sun haɗa da zazzabi, kurji, da lymphadenopathy.
Kwayar cutar kyandar biri tana shiga jikin dan adam ne ta cikin mucosa da karyewar fata. Abubuwan da ke kamuwa da cutar sun hada da kamuwa da cutar sankarau da kuma berayen da suka kamu da cutar, birai, da sauran dabbobin da ba na mutum ba. Bayan kamuwa da cuta, lokacin shiryawa shine kwanaki 5 zuwa 21, yawanci kwanaki 6 zuwa 13.
Duk da cewa yawan jama'a na iya kamuwa da cutar sankarau, amma akwai matakan kariya daga kamuwa da cutar sankarau ga waɗanda aka yi wa rigakafin cutar sankarau, saboda kamanceceniyar kwayoyin halitta da antigenic tsakanin ƙwayoyin cuta. A halin yanzu, cutar sankarau tana yaɗuwa a tsakanin maza waɗanda ke yin jima'i da maza ta hanyar jima'i, yayin da haɗarin kamuwa da cuta ga sauran jama'a ya ragu.
02 Ta yaya Wannan Bullar Biri Ya bambanta?
Tun daga farkon shekara, babban nau'in kwayar cutar kyandar biri, "Clade II," ta haifar da barkewar annoba mai yawa a duniya. Abin damuwa, adadin shari'o'in da "Clade I" ya haifar, wanda ya fi tsanani kuma yana da yawan mace-mace, yana karuwa kuma an tabbatar da shi a wajen nahiyar Afirka. Bugu da ƙari, tun watan Satumbar bara, wani sabon bambance-bambancen, mafi muni da sauƙi, "Clade Ib,” ya fara yaduwa a Jamhuriyar Demokradiyyar Kongo.
Babban abin lura a wannan bullar ita ce mata da yara ‘yan kasa da shekaru 15 ne suka fi kamuwa da cutar.
Bayanai sun nuna cewa sama da kashi 70% na wadanda aka ruwaito suna cikin marasa lafiya ‘yan kasa da shekaru 15, kuma a cikin wadanda suka mutu, wannan adadi ya kai kashi 85%. Musamman,Adadin mace-macen yara ya ninka na manya sau hudu.
03 Menene Haɗarin Yaɗuwar Cutar Cutar Biri?
Saboda lokacin yawon bude ido da yawan cudanya tsakanin kasa da kasa, hadarin kamuwa da kwayar cutar kyandar biri na iya karuwa. Koyaya, kwayar cutar ta fi yaduwa ta hanyar kusanci na dogon lokaci, kamar yin jima'i, saduwa da fata, da numfashi kusa ko yin magana da wasu, don haka ikon watsawa mutum-da-mutum yana da rauni.
04 Yadda Ake Hana Cutar Biri?
Ka guji saduwa da mutanen da ba a san matsayin lafiyarsu ba. Ya kamata matafiya su mai da hankali kan bullar cutar kyandar biri a kasashen da yankunan da suke zuwa, kuma su guji cudanya da rodents da primates.
Idan halayen haɗari mai girma ya faru, kula da lafiyar ku na kwanaki 21 kuma ku guji kusanci da wasu. Idan bayyanar cututtuka irin su kurji, blisters, ko zazzabi sun bayyana, nemi kulawar likita da sauri kuma sanar da likita halayen da suka dace.
Idan wani dangi ko abokinsa ya kamu da cutar sankarau, ɗauki matakan kariya, guje wa kusanci da majiyyaci, kuma kada a taɓa abubuwan da majiyyacin ya yi amfani da su, kamar su tufafi, kwanciya, tawul, da sauran abubuwan sirri. A guji raba banɗaki, kuma akai-akai wanke hannu da shakar dakuna.
Maganin Cutar Cutar Biri
Maganganun binciken cutar sankarau suna taimakawa tabbatar da kamuwa da cuta ta hanyar gano antigens ko ƙwayoyin rigakafin ƙwayoyin cuta, ba da damar keɓe masu dacewa da matakan jiyya, da kuma taka muhimmiyar rawa wajen sarrafa cututtuka masu yaduwa. A halin yanzu, Anhui DeepBlue Medical Technology Co., Ltd. ya ƙirƙira abubuwan gano cutar sankarau mai zuwa:
Kit ɗin Gwajin Antigen Cutar Monkeypox: Yana amfani da hanyar zinari na colloidal don tattara samfura kamar swabs na oropharyngeal, swabs na hanci, ko fitar da fata don ganowa. Yana tabbatar da kamuwa da cuta ta hanyar gano gaban kwayar cutar antigens.
Kit ɗin Gwajin Kwayar Cutar Biri: Yana amfani da hanyar zinari na colloidal, tare da samfurori da suka haɗa da gabaɗayan jini mai jijiya, plasma, ko ruwan magani. Yana tabbatar da kamuwa da cuta ta hanyar gano ƙwayoyin rigakafi da jikin ɗan adam ko dabba ke samarwa daga ƙwayar cuta ta biri.
Kayan Gwajin Kwayoyin Kwayoyin Kwayoyin Kwayoyin Biri: Yana amfani da hanyar PCR mai kyalli na ainihin lokaci, tare da samfurin kasancewar raunuka. Yana tabbatar da kamuwa da cuta ta hanyar gano kwayoyin halittar kwayar cutar ko wasu guntuwar kwayoyin halitta.
Samfuran Gwajin Cutar Kashin Biri na Testsealabs
Tun daga shekara ta 2015, an inganta magungunan gwajin cutar sankarau na Testsealabs ta amfani da samfuran ƙwayoyin cuta na gaske a cikin dakunan gwaje-gwaje na ƙasashen waje kuma an tabbatar da su ta CE saboda kwanciyar hankali da ingantaccen aiki. Wadannan reagents sun yi niyya ga nau'ikan samfuri daban-daban, suna ba da hankali daban-daban da takamaiman matakan, suna ba da tallafi mai ƙarfi don gano kamuwa da cutar sankarau da kuma mafi kyawun taimakawa wajen shawo kan barkewar cutar. Don ƙarin bayani game da kayan gwajin cutar sankarau, da fatan za a bita: https://www.testsealabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Hanyar gwaji
Yin amfani da swab don tattara magudanar ruwa daga pustule, haɗa shi sosai a cikinbuffer, sa'an nan kuma shafa 'yan digo a cikin katin gwaji. Ana iya samun sakamakon a cikin matakai kaɗan kaɗan.