Kwayar cutar Monkeypox (MPV) Kayan Gane Acid Nucleic

Kit ɗin ya ƙunshi reagents don sarrafa gwaje-gwaje 48 ko sarrafa inganci, gami da abubuwan da ke biyowa:

Reagen A

ReagentB

Lura: Abubuwan da aka haɗa na lambobi daban-daban ba za a iya amfani da su ba

【Yanayin Ajiya Da Rayuwar Shelf】

1.Reagent A / B za a iya adana a 2-30 ° C, da kuma shiryayye rayuwa ne 10 watanni.

2.Don Allah a buɗe murfin bututun gwajin kawai lokacin da kuka shirya don gwajin.

3.Kada ku yi amfani da bututun gwaji fiye da ranar karewa.

4.Kada ku yi amfani da bututun ganowa.

【Abubuwan da ake Aiwatar da su】

Dace da dacewa da tsarin bincike na LC480 PCR, Gentier 48E Atomatik PCR tsarin bincike, ABI7500 PCR tsarin bincike.

【Samfuran Bukatun】

1.Amfani nau'in samfurin: samfurin swabs makogwaro.

2.Sampling Magani:Bayan tabbatarwa, ana ba da shawarar yin amfani da saline na yau da kullun ko bututun adana ƙwayar cuta wanda Hangzhou Testsea ilmin halitta ya samar don tarin samfura.

makogwaro:shafa tonsils na pharyngeal biyu da bangon pharyngeal na baya tare da swab samfurin bakararre mai yuwuwa, nutsar da swab cikin bututu mai ɗauke da maganin samfurin 3mL, jefar da wutsiya, sannan ƙara murfin bututu.

3.Sample ajiya da bayarwa:Ya kamata a gwada samfuran da za a gwada da wuri-wuri.The sufuri zafin jiki ya kamata a kiyaye a 2 ~ 8 ℃.The samfurori da za a iya gwada a cikin 24 hours za a iya adana a 2 ℃ ~ 8 ℃ da kuma idan samfurori ba za a iya gwada a cikin 24 hours, shi ya kamata a adana a kasa da ko daidai. zuwa -70 ℃ (idan babu yanayin ajiya na -70 ℃, ana iya adana shi a -20 ℃ na ɗan lokaci), guje wa maimaitawa.

daskarewa da narkewa.

4.Proper samfurin tarin, ajiya, da sufuri suna da mahimmanci ga aikin wannan samfurin.

【Hanyar Gwaji】

1.Sample aiki da samfurin ƙari

1.1 Samfurin sarrafawa

Bayan haɗa maganin samfurin sama tare da samfurori, ɗauki 30μL na samfurin a cikin bututun sakewa na DNA kuma a haɗa shi daidai.

1.2 Loading

Dauki 20μL na reconstitution reagent kuma ƙara shi zuwa MPV gano reagent, ƙara 5μL na sama sarrafa samfurin (The tabbatacce iko da korau iko za a sarrafa a layi daya tare da samfurori), rufe tube hula, centrifuge shi a 2000rpm for 10 seconds.

2. PCR haɓakawa

2.1 Load da farantin PCR da aka shirya / bututu zuwa kayan aikin PCR mai haske, iko mara kyau da ingantaccen iko za a saita don kowane gwaji.

2.2 Saitin tashar Fluorescent:

1) Zaɓi tashar FAM don gano MPV;

2) Zaɓi tashar HEX / VIC don gano abubuwan sarrafawa na ciki;

3.Nazarin sakamako

Sanya layin tushe sama da madaidaicin madaidaicin madaidaicin lanƙwasa mai kyalli.

4.Tsarin sarrafawa

4.1 Korau iko: Babu ƙimar Ct da aka gano a cikin FAM, tashar HEX / VIC, ko Ct ：40;

4.2 Kyakkyawan sarrafawa: A cikin FAM, tashar HEX / VIC, Ct≤40;

4.3 Abubuwan da ke sama yakamata a cika su a cikin gwaji iri ɗaya, in ba haka ba sakamakon gwajin ba shi da inganci kuma gwajin yana buƙatar maimaitawa.

【Yanke darajar】

Ana ɗaukar samfurin a matsayin tabbatacce lokacin: Jerin Target Ct≤40, Tsarin sarrafa ciki Ct≤40.

【Tafsirin sakamako】

Da zarar an wuce ingancin kulawa, masu amfani yakamata su bincika idan akwai madaidaicin haɓakawa ga kowane samfuri a cikin tashar HEX / VIC, idan akwai kuma tare da Ct≤40, yana nuna nasarar haɓakar ƙwayar sarrafawa ta ciki kuma wannan takamaiman gwajin yana da inganci.Masu amfani za su iya ci gaba zuwa bincike mai zuwa:

3.Don samfurori tare da haɓakawa na tsarin kulawa na ciki ya kasa (HEX / VIC

tashar, Ct＞40, ko babu ƙararrawa kwana), ƙananan nauyin ƙwayar cuta ko kasancewar mai hana PCR zai iya zama dalilin rashin nasara, jarrabawar ya kamata a maimaita daga tarin samfurin;

4.Don samfurori masu kyau da ƙwayoyin cuta na al'ada, sakamakon kula da ciki ba ya tasiri;

Don samfuran da aka gwada ba su da kyau, ana buƙatar sarrafa iko na ciki don gwada inganci in ba haka ba sakamakon gabaɗayan ba daidai ba ne kuma ana buƙatar maimaita jarrabawa, farawa daga matakin tarin samfurin.