MonkeyPox Antigen Cassette (Serum/Plasma/Swabs)

Takaitaccen Bayani:

Cutar sankaraucutar zoonotic ce ta kwayar cuta ta hanyar cutarKwayar cutar Monkeypox, wanda nasa neOrthopoxvirus asalinnaPoxviridae iyali. Yayin da yake kama da ƙananan ƙwayar cuta, ƙwayar biri ba ta da ƙarfi kuma tana da ƙarancin mace-mace. An fara gano kwayar cutar a ciki1958a cikin birai na dakin gwaje-gwaje (saboda haka sunan), amma yanzu an san shi da farko yana shafar rodents da sauran dabbobi. An fara ba da rahoton cutar a cikin mutane a cikin1970a cikinJamhuriyar Demokradiyyar Kongo.

Ana iya kamuwa da cutar kyandar biri ga mutane ta hanyar:

Sadarwa kai tsayetare da dabbobi masu kamuwa da cuta (misali, sarrafa naman daji).
Watsawar mutum-da-mutumta ɗigon numfashi, kusanci da ruwan jiki, ko raunukan fata.
Abubuwa masu iya yaa cuta( gurbatattun abubuwa ko filaye).

Alamun cutar kyandar biri a cikin mutane kamar nacutar sankarau, ciki har da:

Zazzaɓi
Ciwon kai
Ciwon tsoka
Gajiya
Rashes, yawanci farawa akan fuska da yaduwa zuwa wasu sassan jiki, sau da yawa suna tasowa zuwa raunuka masu cike da ruwa (pox).

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Cikakken Bayani

Tags samfurin

Cikakken Bayani:

Babban Hankali da Takamaiman
An tsara gwajin don samar da ingantaccen ganowaKwayar cutar Monkeypox antigens ko antibodies, tare da ƙarancin amsawar giciye tare da wasu ƙwayoyin cuta irin wannan.
Sakamako cikin gaggawa
Ana samun sakamako a cikiMinti 15-20, sanya shi manufa don yanke shawara mai sauri a cikisaitunan asibitiko a lokacin barkewar cutar.
Sauƙin Amfani
Gwajin ya dace da mai amfani kuma baya buƙatar horo na musamman ko kayan aiki. Ya dace don amfani da kwararrun kiwon lafiya a wurare daban-daban, gami dadakunan gaggawa, asibitocin waje, kumaasibitocin filin.
Nau'o'in Samfuri iri-iri
Gwajin ya dace daduka jini, magani, koplasma, yana ba da sassauci a cikin tarin samfurin.
Mai šaukuwa kuma Mafi dacewa don Amfani da Filin
Ƙirƙirar ƙirar gwajin ya sa ya dace don amfani a cikisassan lafiya ta hannu, shirye-shiryen wayar da kan al'umma, kumayanayin amsa annoba.

Ka'ida:

TheKit ɗin Gwajin Cutar Cutar Biri Mai Sauriaiki a kan manufa naa kaikaice kwarara immunochromatography, inda gwajin ya gano ko daiKwayar cutar Monkeypox antigens or maganin rigakafi. Tsarin shine kamar haka:

Samfurin Tarin
Ƙananan ƙararduka jini, magani, koplasmaan ƙara zuwa rijiyar samfurin na'urar gwaji. Sannan ana amfani da maganin buffer don sauƙaƙe tafiyar samfurin.
Maganin Antigen-Antibody
Kaset ɗin gwajin ya ƙunshirecombinant antigens or maganin rigakafimusamman ga kwayar cutar Monkeypox. Idan samfurin ya ƙunshi takamaiman ƙwayar cuta ta Monkeypoxmaganin rigakafi(IgM, IgG) koantigensdaga kamuwa da cuta mai aiki, za su ɗaure da abin da ya dace a kan gwajin gwajin.
Hijira na Chromatographic
Samfurin yana motsawa tare da membrane saboda aikin capillary. Idan takamaiman antigens ko ƙwayoyin rigakafin ƙwayoyin cuta na Monkeypox, za su ɗaure da layin gwaji (T line), suna samar da band mai launin bayyane. Har ila yau, motsi na reagents yana tabbatar da samuwar alayin sarrafawa (layin C), wanda ke tabbatar da ingancin gwajin.
Tafsirin sakamako
- Layi biyu (Layin T + C):Kyakkyawan sakamako, yana nuna kasancewar ƙwayar ƙwayar cuta ta Monkeypox antigen ko ƙwayoyin rigakafi.
- Layi ɗaya (Layin C kawai):Sakamako mara kyau, yana nuna babu antigen ko ƙwayoyin rigakafi da za a iya ganowa.
- Babu layi ko layin T kawai:Sakamakon mara inganci, yana buƙatar sake gwadawa.

Abun ciki:

Abun ciki	Adadin	Ƙayyadaddun bayanai
IFU	1	/
Gwada kaset	25	Kowace jakar da aka hatimi mai ɗauke da na'urar gwaji guda ɗaya da na'urar bushewa ɗaya
Diluent na hakar	500μL1 Tube 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Dropper tip	/	/
Swab	25	/

Tsarin Gwaji:


1. Wanke hannu	2. Bincika abubuwan da ke cikin kit kafin gwaji, haɗa da saka fakiti, kaset ɗin gwaji, buffer, swab.	3. Sanya bututun hakar a cikin wurin aiki.	4.peel kashe hatimin tsare-tsare na aluminum daga saman bututun cirewa wanda ke dauke da buffer cirewa.

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5.Ki cire swab a hankali ba tare da taɓa tip ba.Saka gaba ɗaya tip ɗin swab 2 zuwa 3 cm a cikin hancin dama. Lura da tsinkewar hancin hanci. Kuna iya jin haka da yatsun hannu yayin shigar da hancin hanci ko duba. shi a cikin zuciyata. A shafa cikin hancin cikin motsi na madauwari sau 5 na akalla dakika 15,yanzu ki dauko hancin hanci iri daya ki sa a cikin sauran hancin.Swab cikin hancin cikin madauwari sau 5 na akalla dakika 15. Da fatan za a yi gwajin kai tsaye tare da samfurin kuma kada ku yi
barshi tsaye.

6. Sanya swab a cikin bututun hakar. Juya swab na kimanin 10 seconds, Juya swab a kan bututun cirewa, danna kan swab a cikin bututun yayin da yake matse bangarorin bututu don sakin ruwa mai yawa. kamar yadda zai yiwu daga swab.


7. Cire swab daga kunshin ba tare da taɓa padding ba.	8. Mix sosai ta hanyar flicking kasa na bututu. Sanya 3 saukad da samfurin a tsaye a cikin rijiyar samfurin gwajin kaset. Karanta sakamakon bayan minti 15. Lura: Karanta sakamakon a cikin mintuna 20. In ba haka ba, ana ba da shawarar koken gwajin.

Fassarar Sakamako: