Kayan gwajin ICH-CPV-CDV IgG

CANINE INFECTIOUS HEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ICH/CPV/CDV IgG gwajin kit) an tsara shi don kimanta matakan rigakafin kare IgG na ɗan adam na Canine Infectious Hepatitis Virus (ICH), Canine Parvo Virus (CPV) da Cutar Cutar Canine Distemper (CDV).

ABUBUWAN KIT

TSIRA DA KA'IDA

Akwai abubuwa guda biyu da aka haɗa a cikin kowane harsashi: Maɓalli, wanda aka ajiye tare da desiccant a cikin ɗakin ƙasa wanda aka rufe tare da foil na aluminum mai kariya, da kuma samar da mafita, waɗanda aka ajiye su daban a cikin ɗakunan saman da aka rufe tare da kariya ta aluminum.

Kowane harsashi ya ƙunshi duk abubuwan da ake buƙata don gwajin samfurin ɗaya. A taƙaice, lokacin da aka shigar da Maɓalli a ciki kuma a sanya shi na ƴan mintuna a cikin babban ɗakin 1, wanda aka ajiye samfurin jini, takamaiman ƙwayoyin IgG a cikin samfurin jini na diluted, idan akwai, za su ɗaure zuwa ICH, CPV ko CDV recombinant antigens marasa motsi a kan maɓalli daban-daban akan maɓalli da aka saka. Sa'an nan kuma za a mayar da Maɓallin zuwa sauran manyan ɗakunan ajiya a lokaci-lokaci mataki-mataki. Ƙayyadaddun ƙayyadaddun ƙwayoyin rigakafi na IgG a kan tabo za a yi wa lakabi a cikin babban sashi na 3, wanda ya ƙunshi anti-canine IgG enzyme conjugate kuma sakamakon ƙarshe da aka gabatar a matsayin launin shuɗi-blue akan Maɓalli za a haɓaka a saman.

part 6, wanda ya ƙunshi substrate. Don sakamako mai gamsarwa, an gabatar da matakan wankewa. A cikin babban sashin 2, za a cire IgG mara iyaka da sauran abubuwan da ke cikin samfurin jini. A cikin babban sashi na 4 da 5, marasa iyaka ko

wuce haddi anti-canine IgG enzyme conjugate za a iya kawar da isasshe. A ƙarshe, a cikin ɗaki na 7 na sama, za a cire chromosome da suka wuce gona da iri da aka haɓaka daga ƙasa da kuma ɗaure enzyme conjugate a cikin babban sashi na 6. Don tabbatar da ingancin aikin, ana gabatar da furotin mai sarrafawa akan mafi girman tabo akan Maɓalli. Ya kamata a ga tabo a cikin launin shuɗi-shuɗi bayan kammala aikin gwaji mai nasara.

AJIYA

1. Ajiye kayan a ƙarƙashin firiji na yau da kullun (2 ~ 8 ℃).

KAR KU DEKE KIT.

2. Kit ɗin ya ƙunshi kayan halitta marasa aiki. Dole ne a sarrafa kayan

kuma ana zubar da su daidai da buƙatun tsaftar gida.

HANYAR GWADA

Shiri kafin yin gwajin:

1. Kawo harsashi zuwa dakin da zafin jiki (20 ℃-30 ℃) da kuma sanya shi a kan benci na aiki har sai da thermal lakabin a bango na harsashi zama ja launi.

2. Sanya takarda mai tsabta a kan benci na aiki don sanya Maɓalli.

3.Shirya mai rarraba 10μL da 10μL daidaitaccen bututun pipette.

4. Cire foil ɗin aluminum mai kariya na ƙasa kuma jefa Maɓalli daga ɓangaren ƙasa na harsashi a kan takarda mai tsabta.

5. Tsaya a tsaye harsashi akan benci na aiki kuma tabbatar da cewa ana iya ganin manyan lambobi a madaidaiciyar hanya (madaidaicin tambarin lamba yana fuskantar ku). Matsa harsashi kaɗan don tabbatar da mafita a cikin ɗakunan sama sun juya baya zuwa ƙasa.

Yin gwajin:

1.Buɗe foil ɗin kariya a saman ɗakunan a hankali tare da yatsa da yatsa daga hagu zuwa dama har sai kawai fallasa sashin saman 1.

2.Samu samfurin jinin da aka gwada tare da saitin mai rarrabawa ta amfani da daidaitattun 10μL pipette tip.

Don gwajin jini ko plasma, yi amfani da 5 μL.

Don gwada jini gaba ɗaya amfani da 10μL.

Ana ba da shawarar EDTA ko bututun anticoagulant na heparin don plasma da tarin jini gaba ɗaya.

3. Deposit samfurin a cikin saman daki 1. Sa'an nan kuma tada da ƙananan dispenser plunger sau da yawa don cimma hadawa (Light blue bayani a cikin tip lokacin da hadawa yana nuna nasarar samfurin ajiya).

4.Dauki maɓalli ta hannun maɓalli da ɗan yatsa a hankali sannan a saka maɓalli a saman daki na 1 (tabbatar da gefen maɓallin sanyin da ke fuskantar ku, ko tabbatar da cewa Semi-da'irar da ke kan mariƙin yana hannun dama yayin fuskantar ku). ka). Sai ki gauraya ki tsaya Mabudin a saman daki na 1 na minti 5.

5. Cire foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 2. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin fallen da aka fallasa.

6. Cire foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 3. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin fallen da aka fallasa.

7.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 4. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin ɗakin da aka fallasa.

8.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 5. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin ɗakin da aka fallasa.

9.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 6. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin fallen da aka fallasa.

10.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin.

11. Ɗauki Maɓalli daga saman sashin 7 kuma bar shi ya bushe a kan takarda don kimanin minti 5 kafin karanta sakamakon.

Bayanan kula:

Kar a taɓa Gefen Frosting na Gaban Ƙarshen Maɓalli, inda antigens da furotin sarrafawa ba su motsi (Yankin Gwaji da Sarrafa).

Ka guje wa zazzage Yankin Gwaji da Sarrafa ta hanyar jingina wani Siffa mai laushi na gaban ƙarshen Maɓalli zuwa bangon ciki na kowane babban ɗaki yayin haɗuwa.

Don haɗawa, ana ba da shawarar ɗagawa da rage maɓalli sau 10 a kowane babban ɗaki.

KAWAI BAYYANA babban sashi na gaba ɗaya kafin canja wurin Maɓalli.

Idan ya cancanta, haɗa Label ɗin Dabbobin da aka bayar don gwajin samfur fiye da ɗaya.

SAKAMAKON JARRABAWAR FASSARA

Bincika tabo da aka samu akan Maɓalli tare da daidaitaccen launi na launi

Ba daidai ba:

BABU launi mai shuɗi-shuɗi da ake gani yana bayyana akan wurin sarrafawa

Korau(-)

BABU launi mai shuɗi-shuɗi da ke bayyane akan wuraren gwaji

Tabbatacce (+)

Launi mai launin shuɗi-shuɗi yana bayyana akan wuraren gwaji

Ana iya kwatanta titters na takamaiman ƙwayoyin rigakafin IgG ta matakai uku