Ich-cpv-cdv gwajin kit

Cuinine Ciniki na Ciniki / ƙwayar cuta ta Parvo / Wurter / CPV / CDV) an tsara matakan ƙwayoyin cuta na ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta na ƙwayar cuta (ICH), CDIN PARVO Virus (CPV) Kuma cutar da za ta diski (CDV).

Kit abubuwan da ke ciki

Tsarin tsari da ka'idodi

Akwai abubuwan biyu da aka shirya a kowane katangar, maɓallin, wanda aka sanya shi tare da dattawan kayan ado, wanda aka sanya mafita daban, wanda aka ajiye shi daban a cikin manyan kayan kariya tare da ingantaccen kayan kariya.

Kowace cartrge ya ƙunshi duk masu maye gurbin don gwajin samfurin ɗaya. A takaice, lokacin da aka saka maɓallin a ciki kuma an sanya samfurin 'yan mintoci kaɗan a saman samfamar jini, idan ya kasance mai ɗaukar hoto a cikin samfurin ƙwayar cuta, idan ya kasance mai ɗaure ga wannan maganin ich, CPV ko CDV ya juya maganin antigens impbilized akan daban-daban aibobi daban-daban akan mabuɗin da aka saka. Sannan za a canja mabuɗin zuwa sauran manyan kayan haɗin a matakan tsaka-tsaki daga mataki. Za'a iya sanya takamaiman takamaiman IGG a kan wuraren da za a yiwa alama a cikin babban daki 3, wanda ya ƙunshi sakamakon da aka gabatar a cikin mabuɗin mai launin shuɗi a saman

Hada 6, wanda ya ƙunshi substrate. Don sakamako mai gamsarwa, an gabatar da matakai. A cikin manyan strartment 2, iGG igg da sauran abubuwa a cikin samfurin jini za a cire. A cikin babban ɗakin 4 da 5, da ba a kwance ko

Wuce haddi Anti-Canine Igg enzyme conzyme conzyme conzyme conzyme conzyme conzyme conzyme conjugate. A karshen, a cikin manyan dakin 7, wuce gona da iri compomosome ci gaba daga substraste da ɗaure enzyme ta enzyme a cikin saman sittin 6 za a cire. Don tabbatar da ingancin aiki, ana gabatar da furotin iko a kan babba mafi yawan kan mabuɗin. Wajibi ne a bayyane shi mai launin shuɗi-shuɗi bayan kammala aikin gwaji na nasara.

Ajiya

1. Adana kit ɗin a karkashin gyaran gyaran al'ada (2 ~ 8 ℃).

Kar a daskare kit ɗin.

2. Kit ɗin ya ƙunshi kayan aikin halittu. Dole ne a kula da kit ɗin

da kuma zubar da su daidai da bukatun tsabtace gida.

Hanya gwaji

Shiri kafin yin gwajin:

1. Ku kawo cirtar a zazzabi a ɗakin (20 ℃ -30 ℃) kuma sanya shi a kan benci har sai da lakabin aikin a bango ya zama launin ja.

2. Anna takarda mai tsabta akan benci na aiki don sanya mabuɗin.

3.Prepare wani 10μl ya sake amfani da shi na 10μl nasihu.

4. Cire kasuwar kariyar kayan aluminium na aluminum kuma jefa mabuɗin daga cikin dakin karbar kaji a kan takarda nama mai tsabta.

5. Tsaya tsaye a gefen benci kuma tabbatar da cewa za a iya ganin manyan lambobin suttura a cikin shugabanci daidai (daidai lamba mai lamba suna fuskantar ka). Matsa katunan dan kadan don tabbatar da mafita a saman bangarorin juya baya zuwa ƙasa.

Yin gwajin:

1.uncover da kayan kariya a kan manyan bangarori a hankali tare da fannoni da yatsa daga hagu zuwa dama har sai kawai bayyana saman suttura 1.

2. Jaridar Jinin Jinin Jin jini tare da kayan girke-girke na amfani da ƙa'idodin bututun mai 10μL bututun.

Don gwada Magani ko Plasma amfani 5μl.

Don gwada yin amfani da jini duk 10μl.

Edta ko Heparin Antiwulant na Anticoagulant an ba da shawarar don plasma da tarin jini gaba daya.

3. Adana samfurin a cikin babban ɗakin 1

4.Da key ɗin da mabuɗin mabuɗin tare da Forfinger da yatsa a hankali kuma saka maɓallin Mabuɗin a cikin Babban Mabuɗin da ke fuskanta, ko kuma tabbatar da cewa Semi-Circle a kan mai riƙe ku). Sa'an nan kuma a Mix da tsayar da mabuɗin a cikin manyan ɗakin 1 na 5 da minti.

5. Bayyanar da tsare mai kariya ta hanyar dama har sai kawai bayyana mabuɗin a cikin babban ɗakin 2 na minti 2 na minti daya.

6. Bayyana tsarewar kariya har zuwa dama har sai kawai fallasa ma'auni 3

7.uncover da kare tsare gaba zuwa dama har sai dai kawai fallasa strarting 4. Sa'an nan ka ɗauki mabuɗin a cikin babban sashin da aka fallasa 4 na minti 4 na minti 4.

8.UNCover da tsallake tsallake ci gaba zuwa dama har sai dai kawai fallasa ma'auni 5. To, Mix da kuma tsaida makullin a saman ɗakin 5 na minti 5 na minti 1.

9.UNCover da tsallake tsare ya zuwa dama har sai dai kawai fayyace strarting 6. To, Mix da kuma tsaida makullin a saman sakin 6 na 5.

10.UNCover da tsallake tsare yana zuwa dama har sai dai kawai fallasa ma'auni 7

11. Theauki mabuɗin a fitar da saman suttura 7 kuma bari ya bushe a kan takarda na nama don kimanin minti 5 kafin karanta sakamakon.

Bayanan kula:

Kada ku taɓa gefen sanyi na ƙarshen ƙarshen maɓallin, inda antigens da furotin sarrafawa suke da shi (gwaji da yankin sarrafawa).

Guji cin nasarar gwajin da kuma yankin sarrafawa ta hanyar jingin wani gefen wani gefen ƙarshen ƙarshen maɓallin a kan kowane babban ɗakin da ake ciki yayin haɗuwa.

Don haɗawa, sau 10 yana haɓaka da rage maɓallin a cikin kowane babban ɗakin da aka bayar a kowane saman.

Kawai bijirar da babban abu na gaba kafin canja wurin maɓallin.

Idan ya cancanta, haɗa alamun kayan gidan da aka bayar don gwajin samfurin ɗaya.

Fassarar sakamakon gwajin

Duba aibobi da aka sa a kan mabuɗin tare da daidaitaccen launuka

Ba daidai ba:

Babu wani launi mai launin shuɗi-shuɗi yana bayyana akan tabo mai sarrafawa

M(-)

Babu wani launi mai launin shuɗi-shuɗi yana bayyana akan wuraren gwaji

Tabbatacce (+)

Mai haske mai launin shuɗi mai haske yana bayyana akan wuraren gwaji

Takunan takamaiman Igg na rigakafi na iya nuna misalai da matakan uku