FPLVFHVFV Igg gwajin

Parleukopen Pantpeukous / Herpi Virus Earcin Gwajin Gwajin Gwaji (FPLV / FHV Igg Mataki na Peline Virus (FCV).

Kit abubuwan da ke ciki

Tsarin tsari da ka'idodi

Akwai abubuwan biyu da aka shirya a kowane katangar, maɓallin, wanda aka sanya shi tare da dattawan kayan ado, wanda aka sanya mafita daban, wanda aka ajiye shi daban a cikin manyan kayan kariya tare da ingantaccen kayan kariya. Kowace cartrge ya ƙunshi duk masu maye gurbin don gwajin samfurin ɗaya. A takaice, lokacin da aka saka maɓallin a ciki kuma an sanya samfurin 'yan mintoci kaɗan a saman sminadari 1, wanda aka tsara samfurin IGG a cikin samfurin jini, idan ba haka ba, zai ɗaure zuwa FPLV, FHV ko FcV ya juya Antigens immbilized akan daban

A hankali aibobi akan maɓallin da aka saka. Sannan za a canja mabuɗin zuwa sauran manyan kayan haɗin a matakan tsaka-tsaki daga mataki. Za'a sanya takamaiman takamaiman IGG a kan wuraren da za a yiwa alama a saman daki 3, wanda ya ƙunshi wuraren da aka gabatar da shuɗi-shuɗi a saman sintus 6, wanda ya ƙunshi substrate. Don sakamako mai gamsarwa, an gabatar da matakai. A cikin manyan strartment 2, iGG igg da sauran abubuwa a cikin samfurin jini za a cire. A cikin manyan sakin 4 da 5, wanda ba a san shi ba ko wuce haddi na enzyme conjugate sosai. A karshen, a cikin manyan dakin 7, wuce gona da iri compomosome ci gaba daga substraste da ɗaure enzyme ta enzyme a cikin saman sittin 6 za a cire.

Don tabbatar da ingancin aiki, ana gabatar da furotin iko a kan babba mafi yawan kan mabuɗin. Za'a iya zama launi mai launin shuɗi bayan gama aiwatar da nasarar gwajin nasara.

Ajiya

1. Adana kit ɗin a karkashin gyaran gyaran al'ada (2 ~ 8 ℃).

Kar a daskare kit ɗin.

2. Kit ɗin ya ƙunshi kayan aikin halittu. Dole ne a kula da kit ɗin

da kuma zubar da su daidai da bukatun tsabtace gida.

Hanya gwaji

Shiri kafin yin gwajin:

1. Ku kawo cirtar a zazzabi a ɗakin (20 ℃ -30 ℃) kuma sanya shi a kan benci har sai da lakabin aikin a bango ya zama launin ja.

2. Anna takarda mai tsabta akan benci na aiki don sanya mabuɗin.

3.Prepare wani 10μl ya sake amfani da shi na 10μl nasihu.

4. Cire kasuwar kariyar kayan aluminium na aluminum kuma jefa mabuɗin daga cikin dakin karbar kaji a kan takarda nama mai tsabta.

5. Tsaya tsaye a gefen benci kuma tabbatar da cewa za a iya ganin manyan lambobin suttura a cikin shugabanci daidai (daidai lamba mai lamba suna fuskantar ka). Matsa katakan kadan don tabbatar da cewa

mafita a saman bangarorin juya baya zuwa ƙasa.

Yin gwajin:

1.uncover da kayan kariya a kan manyan bangarori a hankali tare da fannoni da yatsa daga hagu zuwa dama har sai kawai bayyana saman suttura 1.

2. Jaridar Jinin Jinin Jin jini tare da kayan girke-girke na amfani da ƙa'idodin bututun mai 10μL bututun.

Don gwada Magani ko Plasma amfani 5μl.

Don gwada yin amfani da jini duk 10μl.

Edta ko Heparin Antiwulant na Anticoagulant an ba da shawarar don plasma da tarin jini gaba daya.

3. Adana samfurin a cikin babban ɗakin 1

4.Da key ɗin da mabuɗin mabuɗin tare da Forfinger da yatsa a hankali kuma saka maɓallin Mabuɗin a cikin Babban Mabuɗin da ke fuskanta, ko kuma tabbatar da cewa Semi-Circle a kan mai riƙe ku). Sa'an nan kuma a Mix da tsayar da mabuɗin a cikin manyan ɗakin 1 na 5 da minti.

5. Bayyana tsarewar kariya a cikin dama har sai dai kawai fallasa Keypartment 2. Ka ɗauki mabuɗin a cikin sashin da aka fallasa 2. Sa'an nan a haɗe da mabuɗin a cikin

Babban ɗakin 2 na minti 1.

6. Uncover da kare tsare gaba zuwa dama har sai kawai bayyana mabuɗin 3. To, Mix da kuma tsaya mabuɗin a cikin

SARKI 3 na mintuna 5.

7.uncover da kare tsare gaba zuwa dama har sai dai kawai fallasa strarting 4. Sa'an nan ka ɗauki mabuɗin a cikin babban sashin da aka fallasa 4 na minti 4 na minti 4.

8.UNCover da tsallake tsallake ci gaba zuwa dama har sai dai kawai fallasa ma'auni 5. To, Mix da kuma tsaida makullin a saman ɗakin 5 na minti 5 na minti 1.

9.UNCover da tsallake tsare ya zuwa dama har sai dai kawai fayyace strarting 6. To, Mix da kuma tsaida makullin a saman sakin 6 na 5.

10.UNCover da tsallake tsare yana zuwa dama har sai dai kawai fallasa ma'auni 7

11. Theauki mabuɗin a fitar da saman suttura 7 kuma bari ya bushe a kan takarda na nama don kimanin minti 5 kafin karanta sakamakon.

 Bayanan kula:

Kada ku taɓa gefen sanyi na ƙarshen ƙarshen maɓallin, inda antigens da furotin sarrafawa suke da shi (gwaji da yankin sarrafawa).

Guji cin nasarar gwajin da kuma yankin sarrafawa ta hanyar jingin wani gefen wani gefen ƙarshen ƙarshen maɓallin a kan kowane babban ɗakin da ake ciki yayin haɗuwa.

Don haɗawa, sau 10 yana haɓaka da rage maɓallin a cikin kowane babban ɗakin da aka bayar a kowane saman.

Kawai bijirar da babban abu na gaba kafin canja wurin maɓallin.

Idan ya cancanta, haɗa alamun kayan gidan da aka bayar don gwajin samfurin ɗaya.

Fassarar sakamakon gwajin

Duba aibobi da aka sa a kan mabuɗin tare da daidaitaccen launuka

Ba daidai ba:

Babu wani launi mai launin shuɗi-shuɗi yana bayyana akan tabo mai sarrafawa

M(-)

Babu wani launi mai launin shuɗi-shuɗi yana bayyana akan wuraren gwaji

Tabbatacce (+)

Mai haske mai launin shuɗi mai haske yana bayyana akan wuraren gwaji

Takunan takamaiman Igg na rigakafi na iya nuna misalai da matakan uku