Kayan gwajin FPLVFHVFCV IgG

FELINE PANLEUKOPENIA/HERPES VIRUS/CALICI VIRUS IgG ANTIBODY TEST KIT (FPLV/FHV/FCV IgG kit) an ƙera shi don ƙididdige adadin matakan rigakafin cat IgG don Feline Panleukopenia (FPLV), Feline Herpes Virus (FHV) da Feline Herpes Virus (FHV) Kwayar cuta (FCV).

ABUBUWAN KIT

TSIRA DA KA'IDA

Akwai abubuwa guda biyu da aka haɗa a cikin kowane harsashi: Maɓalli, wanda aka ajiye tare da desiccant a cikin ɗakin ƙasa wanda aka rufe tare da foil na aluminum mai kariya, da kuma samar da mafita, waɗanda aka ajiye su daban a cikin ɗakunan saman da aka rufe tare da kariya ta aluminum. Kowane harsashi ya ƙunshi duk abubuwan da ake buƙata don gwajin samfurin ɗaya. A taƙaice, lokacin da aka shigar da Maɓalli a ciki kuma a sanya shi na ƴan mintuna a cikin babban sashi na 1, wanda aka ajiye samfurin jini, takamaiman ƙwayoyin IgG a cikin samfurin jinin da aka diluted, idan akwai, za su ɗaure zuwa FPLV, FHV ko FCV recombinant antigens immobilized akan daban-daban

tabo masu hankali akan Maɓallin da aka saka. Sa'an nan kuma za a mayar da Maɓallin zuwa sauran manyan ɗakunan ajiya a lokaci-lokaci mataki-mataki. Ƙayyadadden ƙwayoyin rigakafi na IgG na musamman akan tabo za a yi wa lakabin a cikin babban sashi na 3, wanda ya ƙunshi anti-feline IgG enzyme conjugate kuma sakamakon ƙarshe da aka gabatar a matsayin tabo mai shuɗi-blue za a haɓaka a cikin babban sashin 6, wanda ya ƙunshi substrate. Don sakamako mai gamsarwa, an gabatar da matakan wankewa. A cikin babban sashin 2, za a cire IgG mara iyaka da sauran abubuwan da ke cikin samfurin jini. A cikin babban sashi na 4 da 5, za a kawar da rashin iyaka ko wuce haddi na anti-feline IgG enzyme conjugate. A ƙarshe, a cikin ɗaki na 7 na sama, za a cire chromosome da suka wuce gona da iri da aka haɓaka daga ƙasa da kuma ɗaure enzyme conjugate a cikin babban sashi na 6.

Don tabbatar da ingancin aikin, ana gabatar da furotin mai sarrafawa akan mafi girman tabo akan Maɓalli. Launi mai launin shuɗi-shuɗi ya kamata a bayyane bayan kammala aikin gwaji mai nasara.

AJIYA

1. Ajiye kayan a ƙarƙashin firiji na yau da kullun (2 ~ 8 ℃).

KAR KU DEKE KIT.

2. Kit ɗin ya ƙunshi kayan halitta marasa aiki. Dole ne a sarrafa kayan

kuma ana zubar da su daidai da buƙatun tsaftar gida.

HANYAR GWADA

Shiri kafin yin gwajin:

1. Kawo harsashi zuwa dakin da zafin jiki (20 ℃-30 ℃) da kuma sanya shi a kan benci na aiki har sai da thermal lakabin a bango na harsashi zama ja launi.

2. Sanya takarda mai tsabta a kan benci na aiki don sanya Maɓalli.

3.Shirya mai rarraba 10μL da 10μL daidaitaccen bututun pipette.

4. Cire foil ɗin aluminum mai kariya na ƙasa kuma jefa Maɓalli daga ɓangaren ƙasa na harsashi a kan takarda mai tsabta.

5. Tsaya a tsaye harsashi akan benci na aiki kuma tabbatar da cewa ana iya ganin manyan lambobi a madaidaiciyar hanya (madaidaicin tambarin lamba yana fuskantar ku). Matsa katun don tabbatar da cewa

mafita a cikin ɗakunan sama suna juyawa zuwa ƙasa.

Yin gwajin:

1.Bude foil ɗin kariya a saman ɗakunan a hankali tare da yatsa da yatsa daga hagu zuwa dama har sai kawai a fallasa sashin saman 1.

2.Samu samfurin jinin da aka gwada tare da saitin mai rarrabawa ta amfani da daidaitattun 10μL pipette tip.

Don gwajin jini ko plasma, yi amfani da 5 μL.

Don gwada jini gaba ɗaya amfani da 10μL.

Ana ba da shawarar EDTA ko bututun anticoagulant na heparin don plasma da tarin jini gaba ɗaya.

3. Deposit samfurin a cikin saman daki 1. Sa'an nan kuma tada da ƙananan dispenser plunger sau da yawa don cimma hadawa (Light blue bayani a cikin tip lokacin da hadawa yana nuna nasarar samfurin ajiya).

4.Dauki maɓalli ta hannun maɓalli da ɗan yatsa a hankali sannan a saka maɓalli a saman daki na 1 (tabbatar da gefen maɓallin sanyin da ke fuskantar ku, ko tabbatar da cewa Semi-da'irar da ke kan mariƙin yana hannun dama yayin fuskantar ku). ka). Sai ki gauraya ki tsaya Mabudin a saman daki na 1 na minti 5.

5. Cire foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 2. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin fallen da aka fallasa.

babban sashi 2 don 1 minti.

6. Cire foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 3. Ɗauki maɓalli ta hannun mai riƙe da maɓalli a saka maɓalli a cikin fallen da aka fallasa.

part 3 na minti 5.

7.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 4. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin ɗakin da aka fallasa.

8.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 5. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin ɗakin da aka fallasa.

9.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin 6. Ɗauki maɓalli ta hannun mai riƙe da maɓallin a saka maɓalli a cikin fallen da aka fallasa.

10.Buɗe foil ɗin kariya a ci gaba da zuwa dama har sai kawai a fallasa ɗakin.

11. Ɗauki Maɓalli daga saman sashin 7 kuma bar shi ya bushe a kan takarda don kimanin minti 5 kafin karanta sakamakon.

 Bayanan kula:

Kar a taɓa Gefen Frosting na Gaban Ƙarshen Maɓalli, inda antigens da furotin sarrafawa ba su motsi (Yankin Gwaji da Sarrafa).

Ka guje wa zazzage Yankin Gwaji da Sarrafa ta hanyar jingina wani Siffa mai laushi na gaban ƙarshen Maɓalli zuwa bangon ciki na kowane babban ɗaki yayin haɗuwa.

Don haɗawa, ana ba da shawarar ɗagawa da rage maɓalli sau 10 a kowane babban ɗaki.

KAWAI BAYYANA babban sashi na gaba ɗaya kafin canja wurin Maɓalli.

Idan ya cancanta, haɗa Label ɗin Dabbobin da aka bayar don gwajin samfur fiye da ɗaya.

SAKAMAKON JARRABAWAR FASSARA

Bincika tabo da aka samu akan Maɓalli tare da daidaitaccen launi na launi

Ba daidai ba:

BABU launi mai shuɗi-shuɗi da ake gani yana bayyana akan wurin sarrafawa

Korau(-)

BABU launi mai shuɗi-shuɗi da ke bayyane akan wuraren gwaji

Tabbatacce (+)

Launi mai launin shuɗi-shuɗi yana bayyana akan wuraren gwaji

Ana iya kwatanta titters na takamaiman ƙwayoyin rigakafin IgG ta matakai uku