2020 Good Quality Canine Coronavirus Test Kit - FPLVFHVFCV IgG test kit-google – TESTSEA
2020 Good Quality Canine Coronavirus Test Kit - FPLVFHVFCV IgG test kit-google – TESTSEA Detail:
The FELINE PANLEUKOPENIA/HERPES VIRUS/CALICI VIRUS IgG ANTIBODY TEST KIT (FPLV/FHV/FCV IgG test kit) is designed to semi-quantitatively evaluate cat IgG antibody levels for Feline Panleukopenia (FPLV), Feline Herpes Virus (FHV) and Feline Calici Virus(FCV).
KIT CONTENTS
| Contents |
Quantity |
| Cartridge containing Key and developing solutions |
10 |
| ColorScale |
1 |
| Instruction Manual |
1 |
| Pet Labels |
12 |
DESIGN AND PRINCIPLE
There are two components packaged in each cartridge: Key, which is deposited along with a desiccant in the bottom compartment sealed with a protective aluminum foil, and developing solutions, which are deposited separately in the top compartments sealed with a protective aluminum foil. Each cartridge contains all the necessary reagents for one sample testing. Briefly, when the Key is inserted into and incubated for a few minutes in the top compartment 1, in which a blood sample has been deposited, the specific IgG antibodies in the diluted blood sample, if present, will bind to the FPLV, FHV or FCV recombinant antigens immobilized on different
discrete spots on the inserted Key. Then the Key will be transferred to the remaining top compartments at timed intervals step by step. The bounded specific IgG antibodies on the spots will be labeled in the top compartment 3, which contains anti-feline IgG enzyme conjugate and the final results presented as purple-blue spots will been developed in the top compartment 6, which contains substrate. For a satisfactory result, wash steps are introduced. In the top compartment 2, the unbounded IgG and other substances within the blood sample will be removed. In top compartment 4 and 5, the unbounded or excess anti-feline IgG enzyme conjugate will be adequately eliminated. At the end, in the top compartment 7, the excess chromosome developed from substrate and bounded enzyme conjugate in the top compartment 6 will be removed.
To confirm the validity of a performance, a control protein is introduced on the upper most spot on the Key. A purple-blue color should be visible after finishing a successful testing process.
STORAGE
1. Store the kit under normal refrigeration (2~8℃).
DO NOT FREEZE THE KIT.
2. The kit contains inactivated biological material. The kit must be handled
and disposed of in accordance with local sanitary requirements.
TEST PROCEDURE
Preparation before performing the test:
1. Bring the cartridge to room temperature (20℃-30℃） and place it on the work bench until the thermal label on the wall of the cartridge becomes red color.
2.Place a clean tissue paper on the work bench for placing the Key.
3.Prepare a 10μL dispenser and 10μL standard pipette tips.
4. Remove the bottom protective aluminum foil and cast the Key out of the bottom compartment of the cartridge onto the clean tissue paper.
5. Stand upright the cartridge on the work bench and confirm that the top compartment numbers can be seen in the correct direction (correct number stamps facing you). Tap the cartridge slightly to make sure the
solutions in the top compartments turn back to the bottom.
Performing the test:
1.Uncover the protective foil on top compartments carefully with forefinger and thumb from the left to the right until ONLY exposing the top compartment 1.
2.Obtain the tested blood sample with the dispenser set using a standard 10μL pipette tip.
For testing serum or plasma use 5μL.
For testing whole blood use 10μL.
EDTA or heparin anticoagulant tubes are recommended for plasma and whole blood collection.
3. Deposit the sample into the top compartment 1. Then raise and lower dispenser plunger several times to achieve mixing (Light blue solution in the tip when mixing indicates the successful sample deposit).
4.Pick up the Key by the Key’s Holder with forefinger and thumb carefully and insert the Key into the top compartment 1 (confirm the frosting side of Key facing you, or confirm that the semi-circle on the Holder is on the right when facing you). Then mix and stand the Key in the top compartment 1 for 5 minutes.
5. Uncover the protective foil continuously towards the right until ONLY exposing the compartment 2. Pick up the Key by the Holder and insert the Key into the exposed compartment 2. Then mix and stand the Key in the
top compartment 2 for 1 minute.
6. Uncover the protective foil continuously towards the right until ONLY exposing the compartment 3. Pick up the Key by the Holder and insert the Key into the exposed compartment 3. Then mix and stand the Key in the
compartment 3 for 5 minutes.
7.Uncover the protective foil continuously towards the right until ONLY exposing the compartment 4. Pick up the Key by the Holder and insert the Key into the exposed compartment 4. Then mix and stand the Key in the top compartment 4 for 1 minute.
8.Uncover the protective foil continuously towards the right until ONLY exposing the compartment 5. Pick up the Key by the Holder and insert the Key into the exposed compartment 5. Then mix and stand the Key in the top compartment 5 for 1 minute.
9.Uncover the protective foil continuously towards the right until ONLY exposing the compartment 6. Pick up the Key by the Holder and insert the Key into the exposed compartment 6. Then mix and stand the Key in the top compartment 6 for 5 minutes.
10.Uncover the protective foil continuously towards the right until ONLY exposing the compartment 7. Pick up the Key by the Holder and insert the Key into the exposed compartment 7. Then mix and stand the Key in the top compartment 7 for 1 minute.
11. Take the Key out of the top compartment 7 and let it dry on the tissue paper for approximately 5 minutes before reading the results.
Notes:
Do not touch the Frosting Side of the Front End of the Key, where the antigens and the control protein are immobilized(Test and Control Region).
Avoid scratching the Test and Control Region by leaning the another Smooth Side of the front end of the Key on to the inner wall of each top compartment while mixing.
For mixing, 10 times raising and lowering the Key in each top compartment is recommended.
ONLY Expose the next one top compartment before transferring the Key.
If necessary, attach the provided Pet Labels for more than one sample testing.
INTERPRETING TEST RESULTS
Check the resulted spots on the Key with the standard ColorScale
Invalid:
NO visible purple-blue color appears on control spot
Negative(-)
NO visible purple-blue color appears on test spots
Positive (+)
Visible purple-blue color appears on test spots
The titters of the specific IgG antibodies can be illustrated by three levels
Product detail pictures:
Related Product Guide:
Quality First,and Customer Supreme is our guideline to provide the best service to our customers.Nowadays, we are trying our best to become one of the best exporters in our field to meet customers more need for 2020 Good Quality Canine Coronavirus Test Kit - FPLVFHVFCV IgG test kit-google – TESTSEA, The product will supply to all over the world, such as: Oman, belarus, Switzerland, Our product quality is one of the major concerns and has been produced to meet the customer's standards. "Customer services and relationship" is another important area which we understand good communication and relationships with our customers is the most significant power to run it as a long term business.
By Harriet from Paris - 2018.06.05 13:10
The company can think what our think, the urgency of urgency to act in the interests of our position, can be said this is a responsible company, we had a happy cooperation!
By Margaret from Hongkong - 2018.09.16 11:31